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Inhalation of marijuana affects Drosophila heart function

We investigated the effect of inhalation of vaporized marijuana on cardiac function in Drosophila melanogaster, a suitable genetic model for studying human diseases. Adult flies were exposed to marijuana for variable time periods and the effects on cardiac function were studied. Short treatment prot...

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Autores principales: Gómez, Ivana M., Rodríguez, Maia A., Santalla, Manuela, Kassis, George, Colman Lerner, Jorge E., Aranda, J. Oswaldo, Sedán, Daniela, Andrinolo, Dario, Valverde, Carlos A., Ferrero, Paola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6737967/
https://www.ncbi.nlm.nih.gov/pubmed/31324618
http://dx.doi.org/10.1242/bio.044081
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author Gómez, Ivana M.
Rodríguez, Maia A.
Santalla, Manuela
Kassis, George
Colman Lerner, Jorge E.
Aranda, J. Oswaldo
Sedán, Daniela
Andrinolo, Dario
Valverde, Carlos A.
Ferrero, Paola
author_facet Gómez, Ivana M.
Rodríguez, Maia A.
Santalla, Manuela
Kassis, George
Colman Lerner, Jorge E.
Aranda, J. Oswaldo
Sedán, Daniela
Andrinolo, Dario
Valverde, Carlos A.
Ferrero, Paola
author_sort Gómez, Ivana M.
collection PubMed
description We investigated the effect of inhalation of vaporized marijuana on cardiac function in Drosophila melanogaster, a suitable genetic model for studying human diseases. Adult flies were exposed to marijuana for variable time periods and the effects on cardiac function were studied. Short treatment protocol incremented heart-rate variability. Contractility was augmented only under prolonged exposure to cannabis and it was associated with incremented calcium transient within cardiomyocytes. Neither the activity of the major proteins responsible for calcium handling nor the calcium load of the sarcoplasmic reticulum were affected by the cannabis treatment. The observed changes manifested in the cardiomyocytes even in the absence of the canonical cannabinoid receptors described in mammals. Our results are the first evidence of the in vivo impact of phytocannabinoids in D. melanogaster. By providing a simple and affordable platform prior to mammalian models, this characterization of cardiac function under marijuana exposure opens new paths for conducting genetic screenings using vaporized compounds. This article has an associated First Person interview with the first author of the paper.
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spelling pubmed-67379672019-09-12 Inhalation of marijuana affects Drosophila heart function Gómez, Ivana M. Rodríguez, Maia A. Santalla, Manuela Kassis, George Colman Lerner, Jorge E. Aranda, J. Oswaldo Sedán, Daniela Andrinolo, Dario Valverde, Carlos A. Ferrero, Paola Biol Open Research Article We investigated the effect of inhalation of vaporized marijuana on cardiac function in Drosophila melanogaster, a suitable genetic model for studying human diseases. Adult flies were exposed to marijuana for variable time periods and the effects on cardiac function were studied. Short treatment protocol incremented heart-rate variability. Contractility was augmented only under prolonged exposure to cannabis and it was associated with incremented calcium transient within cardiomyocytes. Neither the activity of the major proteins responsible for calcium handling nor the calcium load of the sarcoplasmic reticulum were affected by the cannabis treatment. The observed changes manifested in the cardiomyocytes even in the absence of the canonical cannabinoid receptors described in mammals. Our results are the first evidence of the in vivo impact of phytocannabinoids in D. melanogaster. By providing a simple and affordable platform prior to mammalian models, this characterization of cardiac function under marijuana exposure opens new paths for conducting genetic screenings using vaporized compounds. This article has an associated First Person interview with the first author of the paper. The Company of Biologists Ltd 2019-07-19 /pmc/articles/PMC6737967/ /pubmed/31324618 http://dx.doi.org/10.1242/bio.044081 Text en © 2019. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Gómez, Ivana M.
Rodríguez, Maia A.
Santalla, Manuela
Kassis, George
Colman Lerner, Jorge E.
Aranda, J. Oswaldo
Sedán, Daniela
Andrinolo, Dario
Valverde, Carlos A.
Ferrero, Paola
Inhalation of marijuana affects Drosophila heart function
title Inhalation of marijuana affects Drosophila heart function
title_full Inhalation of marijuana affects Drosophila heart function
title_fullStr Inhalation of marijuana affects Drosophila heart function
title_full_unstemmed Inhalation of marijuana affects Drosophila heart function
title_short Inhalation of marijuana affects Drosophila heart function
title_sort inhalation of marijuana affects drosophila heart function
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6737967/
https://www.ncbi.nlm.nih.gov/pubmed/31324618
http://dx.doi.org/10.1242/bio.044081
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