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Algicidal bacterium CZBC1 inhibits the growth of Oscillatoria chlorina, Oscillatoria tenuis, and Oscillatoria planctonica

Frequent harmful cyanobacteria blooms limit the sustainable development of aquaculture. Algicidal bacteria can efficiently control harmful algae without secondary pollution. The algicidal bacteria CZBC1 can lyse Oscillatoria spp. and other harmful cyanobacteria, but its effector mechanism and algici...

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Autores principales: Hu, Xiao-Juan, Xu, Yu, Su, Hao-Chang, Xu, Wu-Jie, Wang, Li-Hua, Xu, Yun-Na, Li, Zhuo-Jia, Cao, Yu-Cheng, Wen, Guo-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6738362/
https://www.ncbi.nlm.nih.gov/pubmed/31512077
http://dx.doi.org/10.1186/s13568-019-0872-8
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author Hu, Xiao-Juan
Xu, Yu
Su, Hao-Chang
Xu, Wu-Jie
Wang, Li-Hua
Xu, Yun-Na
Li, Zhuo-Jia
Cao, Yu-Cheng
Wen, Guo-Liang
author_facet Hu, Xiao-Juan
Xu, Yu
Su, Hao-Chang
Xu, Wu-Jie
Wang, Li-Hua
Xu, Yun-Na
Li, Zhuo-Jia
Cao, Yu-Cheng
Wen, Guo-Liang
author_sort Hu, Xiao-Juan
collection PubMed
description Frequent harmful cyanobacteria blooms limit the sustainable development of aquaculture. Algicidal bacteria can efficiently control harmful algae without secondary pollution. The algicidal bacteria CZBC1 can lyse Oscillatoria spp. and other harmful cyanobacteria, but its effector mechanism and algicidal threshold are unknown. In this study, we examined the algicidal effect of CZBC1 on O. chlorina, O. tenuis, and O. planctonica by microscopic enumeration and scanning electron microscopy observation. Then, we examined the alginolytic effects of CZBC1 (concentrations 10(3)–10(6) colony forming units (cfu)/mL) on these three species (concentrations 10(3)–10(6) cells/mL) to determine the effective concentrations of CZBC1 for Oscillatoria spp. alginolysis. Results showed that CZBC1 can directly lyse O. chlorina and O. tenuis but indirectly lyse O. planctonica. When the initial concentration of CZBC1 was 10(6) cfu/mL, alginolytic effects were high for all three species at all concentrations, and the alginolytic rate could reach 100% in 3–9 days. When the initial concentration of CZBC1 was lower (10(3) cfu/mL), its inhibitory effects were delayed by 2–5 days, but the cell counts were significantly decreased compared with the control, evidencing significant alginolysis. In addition, the higher the concentration of the algicidal bacteria suspension, the more significant the alginolytic effects. Our results indicate that CZBC1 has different alginolytic mechanisms for O. chlorina, O. tenuis, and O. planctonica, and that different initial concentrations of CZBC1 have different alginolytic effects on these algal species.
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spelling pubmed-67383622019-09-25 Algicidal bacterium CZBC1 inhibits the growth of Oscillatoria chlorina, Oscillatoria tenuis, and Oscillatoria planctonica Hu, Xiao-Juan Xu, Yu Su, Hao-Chang Xu, Wu-Jie Wang, Li-Hua Xu, Yun-Na Li, Zhuo-Jia Cao, Yu-Cheng Wen, Guo-Liang AMB Express Original Article Frequent harmful cyanobacteria blooms limit the sustainable development of aquaculture. Algicidal bacteria can efficiently control harmful algae without secondary pollution. The algicidal bacteria CZBC1 can lyse Oscillatoria spp. and other harmful cyanobacteria, but its effector mechanism and algicidal threshold are unknown. In this study, we examined the algicidal effect of CZBC1 on O. chlorina, O. tenuis, and O. planctonica by microscopic enumeration and scanning electron microscopy observation. Then, we examined the alginolytic effects of CZBC1 (concentrations 10(3)–10(6) colony forming units (cfu)/mL) on these three species (concentrations 10(3)–10(6) cells/mL) to determine the effective concentrations of CZBC1 for Oscillatoria spp. alginolysis. Results showed that CZBC1 can directly lyse O. chlorina and O. tenuis but indirectly lyse O. planctonica. When the initial concentration of CZBC1 was 10(6) cfu/mL, alginolytic effects were high for all three species at all concentrations, and the alginolytic rate could reach 100% in 3–9 days. When the initial concentration of CZBC1 was lower (10(3) cfu/mL), its inhibitory effects were delayed by 2–5 days, but the cell counts were significantly decreased compared with the control, evidencing significant alginolysis. In addition, the higher the concentration of the algicidal bacteria suspension, the more significant the alginolytic effects. Our results indicate that CZBC1 has different alginolytic mechanisms for O. chlorina, O. tenuis, and O. planctonica, and that different initial concentrations of CZBC1 have different alginolytic effects on these algal species. Springer Berlin Heidelberg 2019-09-11 /pmc/articles/PMC6738362/ /pubmed/31512077 http://dx.doi.org/10.1186/s13568-019-0872-8 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Hu, Xiao-Juan
Xu, Yu
Su, Hao-Chang
Xu, Wu-Jie
Wang, Li-Hua
Xu, Yun-Na
Li, Zhuo-Jia
Cao, Yu-Cheng
Wen, Guo-Liang
Algicidal bacterium CZBC1 inhibits the growth of Oscillatoria chlorina, Oscillatoria tenuis, and Oscillatoria planctonica
title Algicidal bacterium CZBC1 inhibits the growth of Oscillatoria chlorina, Oscillatoria tenuis, and Oscillatoria planctonica
title_full Algicidal bacterium CZBC1 inhibits the growth of Oscillatoria chlorina, Oscillatoria tenuis, and Oscillatoria planctonica
title_fullStr Algicidal bacterium CZBC1 inhibits the growth of Oscillatoria chlorina, Oscillatoria tenuis, and Oscillatoria planctonica
title_full_unstemmed Algicidal bacterium CZBC1 inhibits the growth of Oscillatoria chlorina, Oscillatoria tenuis, and Oscillatoria planctonica
title_short Algicidal bacterium CZBC1 inhibits the growth of Oscillatoria chlorina, Oscillatoria tenuis, and Oscillatoria planctonica
title_sort algicidal bacterium czbc1 inhibits the growth of oscillatoria chlorina, oscillatoria tenuis, and oscillatoria planctonica
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6738362/
https://www.ncbi.nlm.nih.gov/pubmed/31512077
http://dx.doi.org/10.1186/s13568-019-0872-8
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