An inexpensive microscopy system for microfluidic studies in budding yeast

Recently, microfluidic technologies have been developed to allow higher throughput collection of yeast replicative lifespan data. Adoption of these devices has been limited, in part, due to the high cost of the motorized microscopy instrumentation from mainline manufacturers. Inspired by recent deve...

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Autores principales: Chen, Kenneth L., Ven, Toby N., Crane, Matthew M., Chen, Dexter E., Feng, Yen-Chi, Suzuki, Nozomi, Russell, Adam E., de Moraes, Diogo, Kaeberlein, Matt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6738973/
https://www.ncbi.nlm.nih.gov/pubmed/31511839
http://dx.doi.org/10.1016/j.tma.2019.05.001
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author Chen, Kenneth L.
Ven, Toby N.
Crane, Matthew M.
Chen, Dexter E.
Feng, Yen-Chi
Suzuki, Nozomi
Russell, Adam E.
de Moraes, Diogo
Kaeberlein, Matt
author_facet Chen, Kenneth L.
Ven, Toby N.
Crane, Matthew M.
Chen, Dexter E.
Feng, Yen-Chi
Suzuki, Nozomi
Russell, Adam E.
de Moraes, Diogo
Kaeberlein, Matt
author_sort Chen, Kenneth L.
collection PubMed
description Recently, microfluidic technologies have been developed to allow higher throughput collection of yeast replicative lifespan data. Adoption of these devices has been limited, in part, due to the high cost of the motorized microscopy instrumentation from mainline manufacturers. Inspired by recent development of open source microscopy hardware and software, we developed minimal-cost hardware attachments to provide long-term focus stabilization for lower-cost microscopes and open source software to manage concurrent time-lapse image acquisition from multiple microscopes. We hope that these tools will help spur the wider adoption of microfluidic technologies for the study of aging in yeast.
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spelling pubmed-67389732019-09-11 An inexpensive microscopy system for microfluidic studies in budding yeast Chen, Kenneth L. Ven, Toby N. Crane, Matthew M. Chen, Dexter E. Feng, Yen-Chi Suzuki, Nozomi Russell, Adam E. de Moraes, Diogo Kaeberlein, Matt Transl Med Aging Article Recently, microfluidic technologies have been developed to allow higher throughput collection of yeast replicative lifespan data. Adoption of these devices has been limited, in part, due to the high cost of the motorized microscopy instrumentation from mainline manufacturers. Inspired by recent development of open source microscopy hardware and software, we developed minimal-cost hardware attachments to provide long-term focus stabilization for lower-cost microscopes and open source software to manage concurrent time-lapse image acquisition from multiple microscopes. We hope that these tools will help spur the wider adoption of microfluidic technologies for the study of aging in yeast. 2019-06-07 2019 /pmc/articles/PMC6738973/ /pubmed/31511839 http://dx.doi.org/10.1016/j.tma.2019.05.001 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Chen, Kenneth L.
Ven, Toby N.
Crane, Matthew M.
Chen, Dexter E.
Feng, Yen-Chi
Suzuki, Nozomi
Russell, Adam E.
de Moraes, Diogo
Kaeberlein, Matt
An inexpensive microscopy system for microfluidic studies in budding yeast
title An inexpensive microscopy system for microfluidic studies in budding yeast
title_full An inexpensive microscopy system for microfluidic studies in budding yeast
title_fullStr An inexpensive microscopy system for microfluidic studies in budding yeast
title_full_unstemmed An inexpensive microscopy system for microfluidic studies in budding yeast
title_short An inexpensive microscopy system for microfluidic studies in budding yeast
title_sort inexpensive microscopy system for microfluidic studies in budding yeast
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6738973/
https://www.ncbi.nlm.nih.gov/pubmed/31511839
http://dx.doi.org/10.1016/j.tma.2019.05.001
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