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Human renal adipose tissue from normal and tumor kidney: its influence on renal cell carcinoma
Tumor cells can interact with neighboring adipose tissue. We evaluated components present in human adipose explants from normal (hRAN) and kidney cancer (hRAT) tissue, and we evaluated the effects of conditioned media (CMs) from hRAN and hRAT on proliferation, adhesion and migration of tumor and non...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739217/ https://www.ncbi.nlm.nih.gov/pubmed/31534630 http://dx.doi.org/10.18632/oncotarget.27157 |
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author | Bruna, Flavia Alejandra Romeo, Leonardo Rafael Campo-Verde-Arbocco, Fiorella Contador, David Gómez, Silvina Santiano, Flavia Sasso, Corina Verónica Zyla, Leila López-Fontana, Constanza Calvo, Juan Carlos Carón, Rubén Walter Pistone-Creydt, Virginia |
author_facet | Bruna, Flavia Alejandra Romeo, Leonardo Rafael Campo-Verde-Arbocco, Fiorella Contador, David Gómez, Silvina Santiano, Flavia Sasso, Corina Verónica Zyla, Leila López-Fontana, Constanza Calvo, Juan Carlos Carón, Rubén Walter Pistone-Creydt, Virginia |
author_sort | Bruna, Flavia Alejandra |
collection | PubMed |
description | Tumor cells can interact with neighboring adipose tissue. We evaluated components present in human adipose explants from normal (hRAN) and kidney cancer (hRAT) tissue, and we evaluated the effects of conditioned media (CMs) from hRAN and hRAT on proliferation, adhesion and migration of tumor and non-tumor human renal epithelial cell lines. In addition, we evaluated the expression of AdipoR1, ObR, CD44, vimentin, pERK and pPI3K on cell lines incubated with CMs. hRAN were obtained from healthy operated donors, and hRAT from patients who underwent a nephrectomy. hRAT showed increased levels of versican, leptin and ObR; and decreased levels of perilipin, adiponectin and AdipoR1, compared to hRAN. Cell lines showed a significant decrease in cell adhesion and increase in cell migration after incubation with hRAT-CMs vs. hRAN- or control-CMs. Surprisingly, HK-2, 786-O and ACHN cells showed a significant decrease in cell migration after incubation with hRAN-CMs vs. control-CMs. No difference in proliferation of cell lines was found after 24 or 48 h of treatment with CMs. AdipoR1 in ACHN and Caki-1 cells decreased significantly after incubation with hRAT-CMs vs. hRAN-CMs and control-CMs. ObR and CD44 increased in tumor line cells, and vimentin increased in non-tumor cells, after incubation with hRAT-CMs vs. hRAN-CMs and control-CMs. We observed an increase in the expression of pERK and pPI3K in HK-2, 786-O and ACHN, incubated with hRAT-CMs. In conclusion, results showed that adipose microenvironment can regulate the behavior of tumor and non tumor human renal epithelial cells. |
format | Online Article Text |
id | pubmed-6739217 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-67392172019-09-18 Human renal adipose tissue from normal and tumor kidney: its influence on renal cell carcinoma Bruna, Flavia Alejandra Romeo, Leonardo Rafael Campo-Verde-Arbocco, Fiorella Contador, David Gómez, Silvina Santiano, Flavia Sasso, Corina Verónica Zyla, Leila López-Fontana, Constanza Calvo, Juan Carlos Carón, Rubén Walter Pistone-Creydt, Virginia Oncotarget Research Paper Tumor cells can interact with neighboring adipose tissue. We evaluated components present in human adipose explants from normal (hRAN) and kidney cancer (hRAT) tissue, and we evaluated the effects of conditioned media (CMs) from hRAN and hRAT on proliferation, adhesion and migration of tumor and non-tumor human renal epithelial cell lines. In addition, we evaluated the expression of AdipoR1, ObR, CD44, vimentin, pERK and pPI3K on cell lines incubated with CMs. hRAN were obtained from healthy operated donors, and hRAT from patients who underwent a nephrectomy. hRAT showed increased levels of versican, leptin and ObR; and decreased levels of perilipin, adiponectin and AdipoR1, compared to hRAN. Cell lines showed a significant decrease in cell adhesion and increase in cell migration after incubation with hRAT-CMs vs. hRAN- or control-CMs. Surprisingly, HK-2, 786-O and ACHN cells showed a significant decrease in cell migration after incubation with hRAN-CMs vs. control-CMs. No difference in proliferation of cell lines was found after 24 or 48 h of treatment with CMs. AdipoR1 in ACHN and Caki-1 cells decreased significantly after incubation with hRAT-CMs vs. hRAN-CMs and control-CMs. ObR and CD44 increased in tumor line cells, and vimentin increased in non-tumor cells, after incubation with hRAT-CMs vs. hRAN-CMs and control-CMs. We observed an increase in the expression of pERK and pPI3K in HK-2, 786-O and ACHN, incubated with hRAT-CMs. In conclusion, results showed that adipose microenvironment can regulate the behavior of tumor and non tumor human renal epithelial cells. Impact Journals LLC 2019-09-10 /pmc/articles/PMC6739217/ /pubmed/31534630 http://dx.doi.org/10.18632/oncotarget.27157 Text en Copyright: © 2019 Bruna et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Bruna, Flavia Alejandra Romeo, Leonardo Rafael Campo-Verde-Arbocco, Fiorella Contador, David Gómez, Silvina Santiano, Flavia Sasso, Corina Verónica Zyla, Leila López-Fontana, Constanza Calvo, Juan Carlos Carón, Rubén Walter Pistone-Creydt, Virginia Human renal adipose tissue from normal and tumor kidney: its influence on renal cell carcinoma |
title | Human renal adipose tissue from normal and tumor kidney: its influence on renal cell carcinoma |
title_full | Human renal adipose tissue from normal and tumor kidney: its influence on renal cell carcinoma |
title_fullStr | Human renal adipose tissue from normal and tumor kidney: its influence on renal cell carcinoma |
title_full_unstemmed | Human renal adipose tissue from normal and tumor kidney: its influence on renal cell carcinoma |
title_short | Human renal adipose tissue from normal and tumor kidney: its influence on renal cell carcinoma |
title_sort | human renal adipose tissue from normal and tumor kidney: its influence on renal cell carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739217/ https://www.ncbi.nlm.nih.gov/pubmed/31534630 http://dx.doi.org/10.18632/oncotarget.27157 |
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