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The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats

OBJECTIVE: The aim of this animal study was to investigate the short and long-term local histomorphologic effects and the utility of intra-articular application of ibuprofen. METHODS: Forty-six Wistar Albino rats were used in the study. The rats were randomized into 5 groups of 8 and a sham group of...

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Autores principales: Çepni Kütahya, Emine, Oc, Bahar, Ugurluoglu, Ceyhan, Duman, Ipek, Arun, Oguzhan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Turkish Association of Orthopaedics and Traumatology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739263/
https://www.ncbi.nlm.nih.gov/pubmed/30982756
http://dx.doi.org/10.1016/j.aott.2019.03.013
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author Çepni Kütahya, Emine
Oc, Bahar
Ugurluoglu, Ceyhan
Duman, Ipek
Arun, Oguzhan
author_facet Çepni Kütahya, Emine
Oc, Bahar
Ugurluoglu, Ceyhan
Duman, Ipek
Arun, Oguzhan
author_sort Çepni Kütahya, Emine
collection PubMed
description OBJECTIVE: The aim of this animal study was to investigate the short and long-term local histomorphologic effects and the utility of intra-articular application of ibuprofen. METHODS: Forty-six Wistar Albino rats were used in the study. The rats were randomized into 5 groups of 8 and a sham group of 6. The 40 rats in the study groups were anaesthetised with 60 mg/kg of ketamine, then 0.25 ml ibuprofen (25 mg) was injected to the right knee joint of each rat (ibuprofen group) and 0.25 ml 0.9% saline to the left knee joint as the control group. To the 6 rats in the sham group, only puncture was applied to both knee joints. The rats in each of the 5 study groups were sacrificed on days 1, 2, 7, 14 and 21 respectively. The histomorphologic changes were graded on a 6-point scale regarding inflammation of the synovia, cartilage tissue, and subchondral bone. Inflammation scores were compared using the Mann Whitney U-test and comparisons of the sacrifice day and drug used were evaluated with the Kruskal Wallis test. The p values below 0.05 were considered as significant. RESULTS: Statistically significant difference was found between the ibuprofen injected knees (10/40) and the saline injected (0/40) and sham knees (0/12) in respect of hematoma positivity (p = 0.002). Significantly higher inflammation scores were found in ibuprofen injected knees on the 1st, 2nd, 7th and 14th days compared to controls and sham (p < 0.05). Inflammation scores were similar in ibuprofen injected knees with and without hematoma (p > 0.05). Inflammation of the ibuprofen injected group was most severe on day one and the severity of inflammation reduced gradually throughout the 3 weeks. CONCLUSION: Our results show that intra-articular injection of ibuprofen can cause intra-articular hematoma. It also leads to transient inflammation of the synovia that is more severe in the early period, which gradually recovers.
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spelling pubmed-67392632019-09-16 The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats Çepni Kütahya, Emine Oc, Bahar Ugurluoglu, Ceyhan Duman, Ipek Arun, Oguzhan Acta Orthop Traumatol Turc Research Article OBJECTIVE: The aim of this animal study was to investigate the short and long-term local histomorphologic effects and the utility of intra-articular application of ibuprofen. METHODS: Forty-six Wistar Albino rats were used in the study. The rats were randomized into 5 groups of 8 and a sham group of 6. The 40 rats in the study groups were anaesthetised with 60 mg/kg of ketamine, then 0.25 ml ibuprofen (25 mg) was injected to the right knee joint of each rat (ibuprofen group) and 0.25 ml 0.9% saline to the left knee joint as the control group. To the 6 rats in the sham group, only puncture was applied to both knee joints. The rats in each of the 5 study groups were sacrificed on days 1, 2, 7, 14 and 21 respectively. The histomorphologic changes were graded on a 6-point scale regarding inflammation of the synovia, cartilage tissue, and subchondral bone. Inflammation scores were compared using the Mann Whitney U-test and comparisons of the sacrifice day and drug used were evaluated with the Kruskal Wallis test. The p values below 0.05 were considered as significant. RESULTS: Statistically significant difference was found between the ibuprofen injected knees (10/40) and the saline injected (0/40) and sham knees (0/12) in respect of hematoma positivity (p = 0.002). Significantly higher inflammation scores were found in ibuprofen injected knees on the 1st, 2nd, 7th and 14th days compared to controls and sham (p < 0.05). Inflammation scores were similar in ibuprofen injected knees with and without hematoma (p > 0.05). Inflammation of the ibuprofen injected group was most severe on day one and the severity of inflammation reduced gradually throughout the 3 weeks. CONCLUSION: Our results show that intra-articular injection of ibuprofen can cause intra-articular hematoma. It also leads to transient inflammation of the synovia that is more severe in the early period, which gradually recovers. Turkish Association of Orthopaedics and Traumatology 2019-07 2019-04-11 /pmc/articles/PMC6739263/ /pubmed/30982756 http://dx.doi.org/10.1016/j.aott.2019.03.013 Text en © 2019 Turkish Association of Orthopaedics and Traumatology. Publishing services by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Çepni Kütahya, Emine
Oc, Bahar
Ugurluoglu, Ceyhan
Duman, Ipek
Arun, Oguzhan
The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats
title The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats
title_full The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats
title_fullStr The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats
title_full_unstemmed The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats
title_short The effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats
title_sort effects of intra-articular injection of ibuprofen on knee joint cartilage and synovium in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739263/
https://www.ncbi.nlm.nih.gov/pubmed/30982756
http://dx.doi.org/10.1016/j.aott.2019.03.013
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