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Comparative metabolomics of MCF-7 breast cancer cells using different extraction solvents assessed by mass spectroscopy

Metabolic profiling of cancer cells can play a vital role in revealing the molecular bases of cancer development and progression. In this study, gas chromatography coupled with mass spectrometry (GC-MS) was employed for the determination of signatures found in ER+/PR+ breast cancer cells derived fro...

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Autores principales: Semreen, Mohammad H., Alniss, Hasan Y., Grgic, Stefan R., El-Awady, Raafat A., Almehdi, Ahmed H., Mousa, Muath K., Hamoudi, Rifat A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739366/
https://www.ncbi.nlm.nih.gov/pubmed/31511569
http://dx.doi.org/10.1038/s41598-019-49509-y
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author Semreen, Mohammad H.
Alniss, Hasan Y.
Grgic, Stefan R.
El-Awady, Raafat A.
Almehdi, Ahmed H.
Mousa, Muath K.
Hamoudi, Rifat A.
author_facet Semreen, Mohammad H.
Alniss, Hasan Y.
Grgic, Stefan R.
El-Awady, Raafat A.
Almehdi, Ahmed H.
Mousa, Muath K.
Hamoudi, Rifat A.
author_sort Semreen, Mohammad H.
collection PubMed
description Metabolic profiling of cancer cells can play a vital role in revealing the molecular bases of cancer development and progression. In this study, gas chromatography coupled with mass spectrometry (GC-MS) was employed for the determination of signatures found in ER+/PR+ breast cancer cells derived from MCF-7 using different extraction solvents including: A, formic acid in water; B, ammonium hydroxide in water; C, ethyl acetate; D, methanol: water (1:1, v/v); and E, acetonitrile: water (1:1, v/v). The greatest extraction rate and diversity of metabolites occurs with extraction solvents A and E. Extraction solvent D showed moderate extraction efficiency, whereas extraction solvent B and C showed inferior metabolite diversity. Metabolite set enrichment analysis (MSEA) results showed energy production pathways to be key in MCF-7 cell lines. This study showed that mass spectrometry could identify key metabolites associated with cancers. The highest enriched pathways were related to energy production as well as Warburg effect pathways, which may shed light on how energy metabolism has been hijacked to encourage tumour progression and eventually metastasis in breast cancer.
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spelling pubmed-67393662019-09-22 Comparative metabolomics of MCF-7 breast cancer cells using different extraction solvents assessed by mass spectroscopy Semreen, Mohammad H. Alniss, Hasan Y. Grgic, Stefan R. El-Awady, Raafat A. Almehdi, Ahmed H. Mousa, Muath K. Hamoudi, Rifat A. Sci Rep Article Metabolic profiling of cancer cells can play a vital role in revealing the molecular bases of cancer development and progression. In this study, gas chromatography coupled with mass spectrometry (GC-MS) was employed for the determination of signatures found in ER+/PR+ breast cancer cells derived from MCF-7 using different extraction solvents including: A, formic acid in water; B, ammonium hydroxide in water; C, ethyl acetate; D, methanol: water (1:1, v/v); and E, acetonitrile: water (1:1, v/v). The greatest extraction rate and diversity of metabolites occurs with extraction solvents A and E. Extraction solvent D showed moderate extraction efficiency, whereas extraction solvent B and C showed inferior metabolite diversity. Metabolite set enrichment analysis (MSEA) results showed energy production pathways to be key in MCF-7 cell lines. This study showed that mass spectrometry could identify key metabolites associated with cancers. The highest enriched pathways were related to energy production as well as Warburg effect pathways, which may shed light on how energy metabolism has been hijacked to encourage tumour progression and eventually metastasis in breast cancer. Nature Publishing Group UK 2019-09-11 /pmc/articles/PMC6739366/ /pubmed/31511569 http://dx.doi.org/10.1038/s41598-019-49509-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Semreen, Mohammad H.
Alniss, Hasan Y.
Grgic, Stefan R.
El-Awady, Raafat A.
Almehdi, Ahmed H.
Mousa, Muath K.
Hamoudi, Rifat A.
Comparative metabolomics of MCF-7 breast cancer cells using different extraction solvents assessed by mass spectroscopy
title Comparative metabolomics of MCF-7 breast cancer cells using different extraction solvents assessed by mass spectroscopy
title_full Comparative metabolomics of MCF-7 breast cancer cells using different extraction solvents assessed by mass spectroscopy
title_fullStr Comparative metabolomics of MCF-7 breast cancer cells using different extraction solvents assessed by mass spectroscopy
title_full_unstemmed Comparative metabolomics of MCF-7 breast cancer cells using different extraction solvents assessed by mass spectroscopy
title_short Comparative metabolomics of MCF-7 breast cancer cells using different extraction solvents assessed by mass spectroscopy
title_sort comparative metabolomics of mcf-7 breast cancer cells using different extraction solvents assessed by mass spectroscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739366/
https://www.ncbi.nlm.nih.gov/pubmed/31511569
http://dx.doi.org/10.1038/s41598-019-49509-y
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