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A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum
The rapid and accurate diagnosis of Plasmodium falciparum malaria infection is an essential factor in malaria control. Currently, malaria diagnosis in the field depends heavily on using rapid diagnostic tests (RDTs) many of which detect circulating parasite-derived histidine-rich protein 2 antigen (...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739368/ https://www.ncbi.nlm.nih.gov/pubmed/31511562 http://dx.doi.org/10.1038/s41598-019-49389-2 |
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author | Schindler, Tobias Deal, Anna C. Fink, Martina Guirou, Etienne Moser, Kara A. Mwakasungula, Solomon M. Mihayo, Michael G. Jongo, Said A. Chaki, Prosper P. Abdulla, Salim Valverde, Paulo C. Manrique Torres, Katherine Bijeri, Jose R. Silva, Joana C. Hoffman, Stephen L. Gamboa, Dionicia Tanner, Marcel Daubenberger, Claudia |
author_facet | Schindler, Tobias Deal, Anna C. Fink, Martina Guirou, Etienne Moser, Kara A. Mwakasungula, Solomon M. Mihayo, Michael G. Jongo, Said A. Chaki, Prosper P. Abdulla, Salim Valverde, Paulo C. Manrique Torres, Katherine Bijeri, Jose R. Silva, Joana C. Hoffman, Stephen L. Gamboa, Dionicia Tanner, Marcel Daubenberger, Claudia |
author_sort | Schindler, Tobias |
collection | PubMed |
description | The rapid and accurate diagnosis of Plasmodium falciparum malaria infection is an essential factor in malaria control. Currently, malaria diagnosis in the field depends heavily on using rapid diagnostic tests (RDTs) many of which detect circulating parasite-derived histidine-rich protein 2 antigen (PfHRP2) in capillary blood. P. falciparum strains lacking PfHRP2, due to pfhrp2 gene deletions, are an emerging threat to malaria control programs. The novel assay described here, named qHRP2/3-del, is well suited for high-throughput screening of P. falciparum isolates to identify these gene deletions. The qHRP2/3-del assay identified pfhrp2 and pfhrp3 deletion status correctly in 93.4% of samples with parasitemia levels higher than 5 parasites/µL when compared to nested PCR. The qHRP2/3-del assay can correctly identify pfhrp2 and pfhrp3 gene deletions in multiple strain co-infections, particularly prevalent in Sub-Saharan countries. Deployment of this qHRP2/3-del assay will provide rapid insight into the prevalence and potential spread of P. falciparum isolates that escape surveillance by RDTs. |
format | Online Article Text |
id | pubmed-6739368 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67393682019-09-22 A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum Schindler, Tobias Deal, Anna C. Fink, Martina Guirou, Etienne Moser, Kara A. Mwakasungula, Solomon M. Mihayo, Michael G. Jongo, Said A. Chaki, Prosper P. Abdulla, Salim Valverde, Paulo C. Manrique Torres, Katherine Bijeri, Jose R. Silva, Joana C. Hoffman, Stephen L. Gamboa, Dionicia Tanner, Marcel Daubenberger, Claudia Sci Rep Article The rapid and accurate diagnosis of Plasmodium falciparum malaria infection is an essential factor in malaria control. Currently, malaria diagnosis in the field depends heavily on using rapid diagnostic tests (RDTs) many of which detect circulating parasite-derived histidine-rich protein 2 antigen (PfHRP2) in capillary blood. P. falciparum strains lacking PfHRP2, due to pfhrp2 gene deletions, are an emerging threat to malaria control programs. The novel assay described here, named qHRP2/3-del, is well suited for high-throughput screening of P. falciparum isolates to identify these gene deletions. The qHRP2/3-del assay identified pfhrp2 and pfhrp3 deletion status correctly in 93.4% of samples with parasitemia levels higher than 5 parasites/µL when compared to nested PCR. The qHRP2/3-del assay can correctly identify pfhrp2 and pfhrp3 gene deletions in multiple strain co-infections, particularly prevalent in Sub-Saharan countries. Deployment of this qHRP2/3-del assay will provide rapid insight into the prevalence and potential spread of P. falciparum isolates that escape surveillance by RDTs. Nature Publishing Group UK 2019-09-11 /pmc/articles/PMC6739368/ /pubmed/31511562 http://dx.doi.org/10.1038/s41598-019-49389-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Schindler, Tobias Deal, Anna C. Fink, Martina Guirou, Etienne Moser, Kara A. Mwakasungula, Solomon M. Mihayo, Michael G. Jongo, Said A. Chaki, Prosper P. Abdulla, Salim Valverde, Paulo C. Manrique Torres, Katherine Bijeri, Jose R. Silva, Joana C. Hoffman, Stephen L. Gamboa, Dionicia Tanner, Marcel Daubenberger, Claudia A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum |
title | A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum |
title_full | A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum |
title_fullStr | A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum |
title_full_unstemmed | A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum |
title_short | A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum |
title_sort | multiplex qpcr approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of plasmodium falciparum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739368/ https://www.ncbi.nlm.nih.gov/pubmed/31511562 http://dx.doi.org/10.1038/s41598-019-49389-2 |
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