Cargando…

A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum

The rapid and accurate diagnosis of Plasmodium falciparum malaria infection is an essential factor in malaria control. Currently, malaria diagnosis in the field depends heavily on using rapid diagnostic tests (RDTs) many of which detect circulating parasite-derived histidine-rich protein 2 antigen (...

Descripción completa

Detalles Bibliográficos
Autores principales: Schindler, Tobias, Deal, Anna C., Fink, Martina, Guirou, Etienne, Moser, Kara A., Mwakasungula, Solomon M., Mihayo, Michael G., Jongo, Said A., Chaki, Prosper P., Abdulla, Salim, Valverde, Paulo C. Manrique, Torres, Katherine, Bijeri, Jose R., Silva, Joana C., Hoffman, Stephen L., Gamboa, Dionicia, Tanner, Marcel, Daubenberger, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739368/
https://www.ncbi.nlm.nih.gov/pubmed/31511562
http://dx.doi.org/10.1038/s41598-019-49389-2
_version_ 1783450929548230656
author Schindler, Tobias
Deal, Anna C.
Fink, Martina
Guirou, Etienne
Moser, Kara A.
Mwakasungula, Solomon M.
Mihayo, Michael G.
Jongo, Said A.
Chaki, Prosper P.
Abdulla, Salim
Valverde, Paulo C. Manrique
Torres, Katherine
Bijeri, Jose R.
Silva, Joana C.
Hoffman, Stephen L.
Gamboa, Dionicia
Tanner, Marcel
Daubenberger, Claudia
author_facet Schindler, Tobias
Deal, Anna C.
Fink, Martina
Guirou, Etienne
Moser, Kara A.
Mwakasungula, Solomon M.
Mihayo, Michael G.
Jongo, Said A.
Chaki, Prosper P.
Abdulla, Salim
Valverde, Paulo C. Manrique
Torres, Katherine
Bijeri, Jose R.
Silva, Joana C.
Hoffman, Stephen L.
Gamboa, Dionicia
Tanner, Marcel
Daubenberger, Claudia
author_sort Schindler, Tobias
collection PubMed
description The rapid and accurate diagnosis of Plasmodium falciparum malaria infection is an essential factor in malaria control. Currently, malaria diagnosis in the field depends heavily on using rapid diagnostic tests (RDTs) many of which detect circulating parasite-derived histidine-rich protein 2 antigen (PfHRP2) in capillary blood. P. falciparum strains lacking PfHRP2, due to pfhrp2 gene deletions, are an emerging threat to malaria control programs. The novel assay described here, named qHRP2/3-del, is well suited for high-throughput screening of P. falciparum isolates to identify these gene deletions. The qHRP2/3-del assay identified pfhrp2 and pfhrp3 deletion status correctly in 93.4% of samples with parasitemia levels higher than 5 parasites/µL when compared to nested PCR. The qHRP2/3-del assay can correctly identify pfhrp2 and pfhrp3 gene deletions in multiple strain co-infections, particularly prevalent in Sub-Saharan countries. Deployment of this qHRP2/3-del assay will provide rapid insight into the prevalence and potential spread of P. falciparum isolates that escape surveillance by RDTs.
format Online
Article
Text
id pubmed-6739368
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-67393682019-09-22 A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum Schindler, Tobias Deal, Anna C. Fink, Martina Guirou, Etienne Moser, Kara A. Mwakasungula, Solomon M. Mihayo, Michael G. Jongo, Said A. Chaki, Prosper P. Abdulla, Salim Valverde, Paulo C. Manrique Torres, Katherine Bijeri, Jose R. Silva, Joana C. Hoffman, Stephen L. Gamboa, Dionicia Tanner, Marcel Daubenberger, Claudia Sci Rep Article The rapid and accurate diagnosis of Plasmodium falciparum malaria infection is an essential factor in malaria control. Currently, malaria diagnosis in the field depends heavily on using rapid diagnostic tests (RDTs) many of which detect circulating parasite-derived histidine-rich protein 2 antigen (PfHRP2) in capillary blood. P. falciparum strains lacking PfHRP2, due to pfhrp2 gene deletions, are an emerging threat to malaria control programs. The novel assay described here, named qHRP2/3-del, is well suited for high-throughput screening of P. falciparum isolates to identify these gene deletions. The qHRP2/3-del assay identified pfhrp2 and pfhrp3 deletion status correctly in 93.4% of samples with parasitemia levels higher than 5 parasites/µL when compared to nested PCR. The qHRP2/3-del assay can correctly identify pfhrp2 and pfhrp3 gene deletions in multiple strain co-infections, particularly prevalent in Sub-Saharan countries. Deployment of this qHRP2/3-del assay will provide rapid insight into the prevalence and potential spread of P. falciparum isolates that escape surveillance by RDTs. Nature Publishing Group UK 2019-09-11 /pmc/articles/PMC6739368/ /pubmed/31511562 http://dx.doi.org/10.1038/s41598-019-49389-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Schindler, Tobias
Deal, Anna C.
Fink, Martina
Guirou, Etienne
Moser, Kara A.
Mwakasungula, Solomon M.
Mihayo, Michael G.
Jongo, Said A.
Chaki, Prosper P.
Abdulla, Salim
Valverde, Paulo C. Manrique
Torres, Katherine
Bijeri, Jose R.
Silva, Joana C.
Hoffman, Stephen L.
Gamboa, Dionicia
Tanner, Marcel
Daubenberger, Claudia
A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum
title A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum
title_full A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum
title_fullStr A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum
title_full_unstemmed A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum
title_short A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum
title_sort multiplex qpcr approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of plasmodium falciparum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739368/
https://www.ncbi.nlm.nih.gov/pubmed/31511562
http://dx.doi.org/10.1038/s41598-019-49389-2
work_keys_str_mv AT schindlertobias amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT dealannac amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT finkmartina amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT guirouetienne amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT moserkaraa amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT mwakasungulasolomonm amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT mihayomichaelg amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT jongosaida amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT chakiprosperp amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT abdullasalim amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT valverdepaulocmanrique amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT torreskatherine amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT bijerijoser amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT silvajoanac amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT hoffmanstephenl amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT gamboadionicia amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT tannermarcel amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT daubenbergerclaudia amultiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT schindlertobias multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT dealannac multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT finkmartina multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT guirouetienne multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT moserkaraa multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT mwakasungulasolomonm multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT mihayomichaelg multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT jongosaida multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT chakiprosperp multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT abdullasalim multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT valverdepaulocmanrique multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT torreskatherine multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT bijerijoser multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT silvajoanac multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT hoffmanstephenl multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT gamboadionicia multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT tannermarcel multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum
AT daubenbergerclaudia multiplexqpcrapproachfordetectionofpfhrp2andpfhrp3genedeletionsinmultiplestraininfectionsofplasmodiumfalciparum