Cargando…

Selection of reference genes for measuring the expression of aiiO in Ochrobactrum quorumnocens A44 using RT-qPCR

Reverse transcription quantitative PCR (RT-qPCR), a method of choice for quantification of gene expression changes, requires stably expressed reference genes for normalization of data. So far, no reference genes were established for the Alphaproteobacteria of the genus Ochrobactrum. Here, we determi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Krzyżanowska, Dorota M., Supernat, Anna, Maciąg, Tomasz, Matuszewska, Marta, Jafra, Sylwia
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739375/ https://www.ncbi.nlm.nih.gov/pubmed/31511547 http://dx.doi.org/10.1038/s41598-019-49474-6

Ejemplares similares

Ochrobactrum quorumnocens sp. nov., a quorum quenching bacterium from the potato rhizosphere, and comparative genome analysis with related type strains
por: Krzyżanowska, Dorota M., et al.
Publicado: (2019)

Correction: Ochrobactrum quorumnocens sp. nov., a quorum quenching bacterium from the potato rhizosphere, and comparative genome analysis with related type strains
por: Krzyżanowska, Dorota M., et al.
Publicado: (2019)

Improving the production of AHL lactonase AiiO-AIO6 from Ochrobactrum sp. M231 in intracellular protease-deficient Bacillus subtilis
por: Xia, Rui, et al.
Publicado: (2020)

Colonization of Potato Rhizosphere by GFP-Tagged Bacillus subtilis MB73/2, Pseudomonas sp. P482 and Ochrobactrum sp. A44 Shown on Large Sections of Roots Using Enrichment Sample Preparation and Confocal Laser Scanning Microscopy
por: Krzyzanowska, Dorota, et al.
Publicado: (2012)

Tutorial: Guidelines for Single-Cell RT-qPCR
por: Zucha, Daniel, et al.
Publicado: (2021)

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
por: Fout, G. Shay, et al.
Publicado: (2016)

Algorithm Optimization in Methylation Detection with Multiple RT-qPCR
por: Song, Lele, et al.
Publicado: (2016)

Improving the standardization of mRNA measurement by RT-qPCR
por: Sanders, Rebecca, et al.
Publicado: (2018)

COVID-19 diagnosis by RT-qPCR in alternative specimens
por: Gonçalves, Cássia Cristina Alves, et al.
Publicado: (2021)

Rapid and accurate quantification of isomiRs by RT-qPCR
por: Franco, Sandra, et al.
Publicado: (2022)

The Great Five—an artificial bacterial consortium with antagonistic activity towards Pectobacterium spp. and Dickeya spp.: formulation, shelf life, and the ability to prevent soft rot of potato in storage
por: Maciag, Tomasz, et al.
Publicado: (2020)

Evaluation of the mechanism of action of Bacillus spp. to manage Meloidogyne incognita with split root assay, RT-qPCR and qPCR
por: Gattoni, Kaitlin M., et al.
Publicado: (2023)

SASqPCR: Robust and Rapid Analysis of RT-qPCR Data in SAS
por: Ling, Daijun
Publicado: (2012)

Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA
por: Park, Changwoo, et al.
Publicado: (2023)

Detection and identification of potentially infectious gastrointestinal and respiratory viruses at workplaces of wastewater treatment plants with viability qPCR/RT-qPCR
por: Stobnicka-Kupiec, Agata, et al.
Publicado: (2022)

Selection of Suitable Reference Genes for RT-qPCR Analyses in Cyanobacteria
por: Pinto, Filipe, et al.
Publicado: (2012)

Applicability of RNA standards for evaluating RT-qPCR assays and platforms
por: Devonshire, Alison S, et al.
Publicado: (2011)

RT-qPCR Normalization Genes in the Red Alga Chondrus crispus
por: Kowalczyk, Nathalie, et al.
Publicado: (2014)

Hepatitis A virus subgenotyping based on RT-qPCR assays
por: Coudray-Meunier, Coralie, et al.
Publicado: (2014)

Custom RT-qPCR-array for glaucoma filtering surgery prognosis
por: Rodriguez-Agirretxe, Iñaki, et al.
Publicado: (2017)

Linear-hairpin variable primer RT-qPCR for MicroRNA
por: Lan, Lin, et al.
Publicado: (2018)

Predictive factors of COVID-19 in patients with negative RT-qPCR
por: López de la Iglesia, J., et al.
Publicado: (2020)

Large-Scale RT-qPCR Diagnostics for Seed Potato Certification
por: Schumpp, Olivier, et al.
Publicado: (2021)

Sampling, Logistics, and Analytics of Urine for RT-qPCR-based Diagnostics
por: Kretschmer-Kazemi Far, Rosel, et al.
Publicado: (2021)

RT-qPCR-based pool testing for the diagnosis of COVID-19
por: Sato, Hugo Itaru, et al.
Publicado: (2023)

Detection of Cytoplasmic and Nuclear Circular RNA via RT-qPCR
por: Tan, Ke-En, et al.
Publicado: (2023)

Development of RT-qPCR and semi-nested RT-PCR assays for molecular diagnosis of hantavirus pulmonary syndrome
por: Nunes, Bruno Tardelli Diniz, et al.
Publicado: (2019)

NormQ: RNASeq normalization based on RT-qPCR derived size factors
por: Naraine, Ravindra, et al.
Publicado: (2020)

Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
por: Paiva, Marcelo Henrique Santos, et al.
Publicado: (2017)

Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater
por: Ciesielski, Mark, et al.
Publicado: (2021)

Validation of reference genes as an internal control for studying Avena sativa–Puccinia coronata interaction by RT-qPCR
por: Sowa, Sylwia, et al.
Publicado: (2022)

The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482
por: Matuszewska, Marta, et al.
Publicado: (2021)

Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR
por: Shrestha, Kiran, et al.
Publicado: (2023)

Reverse transcription-quantitative PCR (RT-qPCR) without the need for prior removal of DNA
por: Đermić, Damir, et al.
Publicado: (2023)

A nationwide analytical and clinical evaluation of 44 rapid antigen tests for SARS-CoV-2 compared to RT-qPCR
por: Schneider, Uffe Vest, et al.
Publicado: (2022)

Accurate RT-qPCR gene expression analysis on cell culture lysates
por: Van Peer, Gert, et al.
Publicado: (2012)

A reliable method for quantification of splice variants using RT-qPCR
por: Camacho Londoño, Julia, et al.
Publicado: (2016)

Assessment of brain reference genes for RT-qPCR studies in neurodegenerative diseases
por: Rydbirk, Rasmus, et al.
Publicado: (2016)

Assessment of suitable reference genes for RT–qPCR studies in chronic rhinosinusitis
por: Nakayama, Tsuguhisa, et al.
Publicado: (2018)

Eprobe mediated RT-qPCR for the detection of leukemia-associated fusion genes
por: Tsuchiya, Koji, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_j720do413ud6o7t4ulvs6q8m79