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Genomic Variation among Strains of Crithidia bombi and C. expoeki
In this study, we sequenced and analyzed the genomes of 40 strains, in addition to the already-reported two type strains, of two Crithidia species infecting bumblebees in Alaska and Central Europe and demonstrated that different strains of Crithidia bombi and C. expoeki vary considerably in terms of...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739494/ https://www.ncbi.nlm.nih.gov/pubmed/31511368 http://dx.doi.org/10.1128/mSphere.00482-19 |
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author | Gerasimov, Evgeny Zemp, Niklaus Schmid-Hempel, Regula Schmid-Hempel, Paul Yurchenko, Vyacheslav |
author_facet | Gerasimov, Evgeny Zemp, Niklaus Schmid-Hempel, Regula Schmid-Hempel, Paul Yurchenko, Vyacheslav |
author_sort | Gerasimov, Evgeny |
collection | PubMed |
description | In this study, we sequenced and analyzed the genomes of 40 strains, in addition to the already-reported two type strains, of two Crithidia species infecting bumblebees in Alaska and Central Europe and demonstrated that different strains of Crithidia bombi and C. expoeki vary considerably in terms of single nucleotide polymorphisms and gene copy number. Based on the genomic structure, phylogenetic analyses, and the pattern of copy number variation, we confirmed the status of C. expoeki as a separate species. The Alaskan populations appear to be clearly separated from those of Central Europe. This pattern fits a scenario of rapid host-parasite coevolution, where the selective advantage of a given parasite strain is only temporary. This study provides helpful insights into possible scenarios of selection and diversification of trypanosomatid parasites. IMPORTANCE A group of trypanosomatid flagellates includes several well-studied medically and economically important parasites of vertebrates and plants. Nevertheless, the vast majority of trypanosomatids infect only insects (mostly flies and true bugs) and, because of that, has attracted little research attention in the past. Of several hundred trypanosomatid species, only four can infect bees (honeybees and bumblebees). Because of such scarcity, these parasites are severely understudied. We analyzed whole-genome information for a total of 42 representatives of bee-infecting trypanosomatids collected in Central Europe and Alaska from a population genetics point of view. Our data shed light on the evolution, selection, and diversification in this important group of trypanosomatid parasites. |
format | Online Article Text |
id | pubmed-6739494 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-67394942019-09-13 Genomic Variation among Strains of Crithidia bombi and C. expoeki Gerasimov, Evgeny Zemp, Niklaus Schmid-Hempel, Regula Schmid-Hempel, Paul Yurchenko, Vyacheslav mSphere Research Article In this study, we sequenced and analyzed the genomes of 40 strains, in addition to the already-reported two type strains, of two Crithidia species infecting bumblebees in Alaska and Central Europe and demonstrated that different strains of Crithidia bombi and C. expoeki vary considerably in terms of single nucleotide polymorphisms and gene copy number. Based on the genomic structure, phylogenetic analyses, and the pattern of copy number variation, we confirmed the status of C. expoeki as a separate species. The Alaskan populations appear to be clearly separated from those of Central Europe. This pattern fits a scenario of rapid host-parasite coevolution, where the selective advantage of a given parasite strain is only temporary. This study provides helpful insights into possible scenarios of selection and diversification of trypanosomatid parasites. IMPORTANCE A group of trypanosomatid flagellates includes several well-studied medically and economically important parasites of vertebrates and plants. Nevertheless, the vast majority of trypanosomatids infect only insects (mostly flies and true bugs) and, because of that, has attracted little research attention in the past. Of several hundred trypanosomatid species, only four can infect bees (honeybees and bumblebees). Because of such scarcity, these parasites are severely understudied. We analyzed whole-genome information for a total of 42 representatives of bee-infecting trypanosomatids collected in Central Europe and Alaska from a population genetics point of view. Our data shed light on the evolution, selection, and diversification in this important group of trypanosomatid parasites. American Society for Microbiology 2019-09-11 /pmc/articles/PMC6739494/ /pubmed/31511368 http://dx.doi.org/10.1128/mSphere.00482-19 Text en Copyright © 2019 Gerasimov et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Gerasimov, Evgeny Zemp, Niklaus Schmid-Hempel, Regula Schmid-Hempel, Paul Yurchenko, Vyacheslav Genomic Variation among Strains of Crithidia bombi and C. expoeki |
title | Genomic Variation among Strains of Crithidia bombi and C. expoeki |
title_full | Genomic Variation among Strains of Crithidia bombi and C. expoeki |
title_fullStr | Genomic Variation among Strains of Crithidia bombi and C. expoeki |
title_full_unstemmed | Genomic Variation among Strains of Crithidia bombi and C. expoeki |
title_short | Genomic Variation among Strains of Crithidia bombi and C. expoeki |
title_sort | genomic variation among strains of crithidia bombi and c. expoeki |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739494/ https://www.ncbi.nlm.nih.gov/pubmed/31511368 http://dx.doi.org/10.1128/mSphere.00482-19 |
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