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A simple model for determining affinity from irreversible thermal shifts
Thermal denaturation (Tm) data are easy to obtain; it is a technique that is used by both small labs and large‐scale industrial organizations. The link between ligand affinity (K (D)) and ΔTm is understood for reversible denaturation; however, there is a gap in our understanding of how to quantitati...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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John Wiley & Sons, Inc.
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739816/ https://www.ncbi.nlm.nih.gov/pubmed/31361943 http://dx.doi.org/10.1002/pro.3701 |