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Characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain BP-2

OBJECTIVES: Bifunctional alginate lyase can efficiently saccharify alginate biomass and prepare functional oligosaccharides of alginate. RESULTS: A new BP-2 strain that produces alginate lyase was screened and identified from rotted Sargassum. A new alginate lyase, Alg17B, belonging to the polysacch...

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Autores principales: Huang, Guiyuan, Wen, Shunhua, Liao, Siming, Wang, Qiaozhen, Pan, Shihan, Zhang, Rongcan, Lei, Fu, Liao, Wei, Feng, Jie, Huang, Shushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6742608/
https://www.ncbi.nlm.nih.gov/pubmed/31418101
http://dx.doi.org/10.1007/s10529-019-02722-1
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author Huang, Guiyuan
Wen, Shunhua
Liao, Siming
Wang, Qiaozhen
Pan, Shihan
Zhang, Rongcan
Lei, Fu
Liao, Wei
Feng, Jie
Huang, Shushi
author_facet Huang, Guiyuan
Wen, Shunhua
Liao, Siming
Wang, Qiaozhen
Pan, Shihan
Zhang, Rongcan
Lei, Fu
Liao, Wei
Feng, Jie
Huang, Shushi
author_sort Huang, Guiyuan
collection PubMed
description OBJECTIVES: Bifunctional alginate lyase can efficiently saccharify alginate biomass and prepare functional oligosaccharides of alginate. RESULTS: A new BP-2 strain that produces alginate lyase was screened and identified from rotted Sargassum. A new alginate lyase, Alg17B, belonging to the polysaccharide lyase family 17, was isolated and purified from BP-2 fermentation broth by freeze-drying, dialysis, and ion exchange chromatography. The enzymatic properties of the purified lyase were investigated. The molecular weight of Alg17B was approximately 77 kDa, its optimum reaction temperature was 40–45 °C, and its optimum reaction pH was 7.5–8.0. The enzyme was relatively stable at pH 7.0–8.0, with a temperature range of 25–35 °C, and the specific activity of the purified enzyme reached 4036 U/mg. A low Na(+) concentration stimulated Alg17B enzyme activity, but Ca(2+), Zn(2+), and other metal ions inhibited it. Substrate specificity analysis, thin-layer chromatography, and mass spectrometry showed that Alg17B is an alginate lyase that catalyses the hydrolysis of sodium alginate, polymannuronic acid (polyM) and polyguluronic acid to produce monosaccharides and low molecular weight oligosaccharides. Alg17B is also bifunctional, exhibiting both endolytic and exolytic activities toward alginate, and has a wide substrate utilization range with a preference for polyM. CONCLUSIONS: Alg17B can be used to saccharify the main carbohydrate, alginate, in the ethanolic production of brown algae fuel as well as in preparing and researching oligosaccharides.
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spelling pubmed-67426082019-09-27 Characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain BP-2 Huang, Guiyuan Wen, Shunhua Liao, Siming Wang, Qiaozhen Pan, Shihan Zhang, Rongcan Lei, Fu Liao, Wei Feng, Jie Huang, Shushi Biotechnol Lett Original Research Paper OBJECTIVES: Bifunctional alginate lyase can efficiently saccharify alginate biomass and prepare functional oligosaccharides of alginate. RESULTS: A new BP-2 strain that produces alginate lyase was screened and identified from rotted Sargassum. A new alginate lyase, Alg17B, belonging to the polysaccharide lyase family 17, was isolated and purified from BP-2 fermentation broth by freeze-drying, dialysis, and ion exchange chromatography. The enzymatic properties of the purified lyase were investigated. The molecular weight of Alg17B was approximately 77 kDa, its optimum reaction temperature was 40–45 °C, and its optimum reaction pH was 7.5–8.0. The enzyme was relatively stable at pH 7.0–8.0, with a temperature range of 25–35 °C, and the specific activity of the purified enzyme reached 4036 U/mg. A low Na(+) concentration stimulated Alg17B enzyme activity, but Ca(2+), Zn(2+), and other metal ions inhibited it. Substrate specificity analysis, thin-layer chromatography, and mass spectrometry showed that Alg17B is an alginate lyase that catalyses the hydrolysis of sodium alginate, polymannuronic acid (polyM) and polyguluronic acid to produce monosaccharides and low molecular weight oligosaccharides. Alg17B is also bifunctional, exhibiting both endolytic and exolytic activities toward alginate, and has a wide substrate utilization range with a preference for polyM. CONCLUSIONS: Alg17B can be used to saccharify the main carbohydrate, alginate, in the ethanolic production of brown algae fuel as well as in preparing and researching oligosaccharides. Springer Netherlands 2019-08-16 2019 /pmc/articles/PMC6742608/ /pubmed/31418101 http://dx.doi.org/10.1007/s10529-019-02722-1 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Research Paper
Huang, Guiyuan
Wen, Shunhua
Liao, Siming
Wang, Qiaozhen
Pan, Shihan
Zhang, Rongcan
Lei, Fu
Liao, Wei
Feng, Jie
Huang, Shushi
Characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain BP-2
title Characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain BP-2
title_full Characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain BP-2
title_fullStr Characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain BP-2
title_full_unstemmed Characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain BP-2
title_short Characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain BP-2
title_sort characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain bp-2
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6742608/
https://www.ncbi.nlm.nih.gov/pubmed/31418101
http://dx.doi.org/10.1007/s10529-019-02722-1
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