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Pollen-derived RNAs Are Found in the Human Circulation

The presence of nonhuman RNAs in man has been questioned and it is unclear if food-derived miRNAs cross into the circulation. In a large population study, we found nonhuman miRNAs in plasma by RNA sequencing and validated a small number of pine-pollen miRNAs by RT-qPCR in 2,776 people. The presence...

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Autores principales: Koupenova, Milka, Mick, Eric, Corkrey, Heather A., Singh, Anupama, Tanriverdi, Selim E., Vitseva, Olga, Levy, Daniel, Keeler, Allison M., Ezzaty Mirhashemi, Marzieh, ElMallah, Mai K., Gerstein, Mark, Rozowsky, Joel, Tanriverdi, Kahraman, Freedman, Jane E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6742912/
https://www.ncbi.nlm.nih.gov/pubmed/31518900
http://dx.doi.org/10.1016/j.isci.2019.08.035
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author Koupenova, Milka
Mick, Eric
Corkrey, Heather A.
Singh, Anupama
Tanriverdi, Selim E.
Vitseva, Olga
Levy, Daniel
Keeler, Allison M.
Ezzaty Mirhashemi, Marzieh
ElMallah, Mai K.
Gerstein, Mark
Rozowsky, Joel
Tanriverdi, Kahraman
Freedman, Jane E.
author_facet Koupenova, Milka
Mick, Eric
Corkrey, Heather A.
Singh, Anupama
Tanriverdi, Selim E.
Vitseva, Olga
Levy, Daniel
Keeler, Allison M.
Ezzaty Mirhashemi, Marzieh
ElMallah, Mai K.
Gerstein, Mark
Rozowsky, Joel
Tanriverdi, Kahraman
Freedman, Jane E.
author_sort Koupenova, Milka
collection PubMed
description The presence of nonhuman RNAs in man has been questioned and it is unclear if food-derived miRNAs cross into the circulation. In a large population study, we found nonhuman miRNAs in plasma by RNA sequencing and validated a small number of pine-pollen miRNAs by RT-qPCR in 2,776 people. The presence of these pine-pollen miRNAs associated with hay fever and not with overt cardiovascular or pulmonary disease. Using in vivo and in vitro models, we found that transmission of pollen-miRNAs into the circulation occurs via pulmonary transfer and this transfer was mediated by platelet-pulmonary vascular cell interactions and platelet pollen-DNA uptake. These data demonstrate that pollen-derived plant miRNAs can be horizontally transferred into the circulation via the pulmonary system in humans. Although these data suggest mechanistic plausibility for pulmonary-mediated plant-derived miRNA transfer into the human circulation, our large observational cohort data do not implicate major disease or risk factor association.
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spelling pubmed-67429122019-09-16 Pollen-derived RNAs Are Found in the Human Circulation Koupenova, Milka Mick, Eric Corkrey, Heather A. Singh, Anupama Tanriverdi, Selim E. Vitseva, Olga Levy, Daniel Keeler, Allison M. Ezzaty Mirhashemi, Marzieh ElMallah, Mai K. Gerstein, Mark Rozowsky, Joel Tanriverdi, Kahraman Freedman, Jane E. iScience Article The presence of nonhuman RNAs in man has been questioned and it is unclear if food-derived miRNAs cross into the circulation. In a large population study, we found nonhuman miRNAs in plasma by RNA sequencing and validated a small number of pine-pollen miRNAs by RT-qPCR in 2,776 people. The presence of these pine-pollen miRNAs associated with hay fever and not with overt cardiovascular or pulmonary disease. Using in vivo and in vitro models, we found that transmission of pollen-miRNAs into the circulation occurs via pulmonary transfer and this transfer was mediated by platelet-pulmonary vascular cell interactions and platelet pollen-DNA uptake. These data demonstrate that pollen-derived plant miRNAs can be horizontally transferred into the circulation via the pulmonary system in humans. Although these data suggest mechanistic plausibility for pulmonary-mediated plant-derived miRNA transfer into the human circulation, our large observational cohort data do not implicate major disease or risk factor association. Elsevier 2019-08-24 /pmc/articles/PMC6742912/ /pubmed/31518900 http://dx.doi.org/10.1016/j.isci.2019.08.035 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Koupenova, Milka
Mick, Eric
Corkrey, Heather A.
Singh, Anupama
Tanriverdi, Selim E.
Vitseva, Olga
Levy, Daniel
Keeler, Allison M.
Ezzaty Mirhashemi, Marzieh
ElMallah, Mai K.
Gerstein, Mark
Rozowsky, Joel
Tanriverdi, Kahraman
Freedman, Jane E.
Pollen-derived RNAs Are Found in the Human Circulation
title Pollen-derived RNAs Are Found in the Human Circulation
title_full Pollen-derived RNAs Are Found in the Human Circulation
title_fullStr Pollen-derived RNAs Are Found in the Human Circulation
title_full_unstemmed Pollen-derived RNAs Are Found in the Human Circulation
title_short Pollen-derived RNAs Are Found in the Human Circulation
title_sort pollen-derived rnas are found in the human circulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6742912/
https://www.ncbi.nlm.nih.gov/pubmed/31518900
http://dx.doi.org/10.1016/j.isci.2019.08.035
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