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Quantitative MNase-seq accurately maps nucleosome occupancy levels
Micrococcal nuclease (MNase) is widely used to map nucleosomes. However, its aggressive endo-/exo-nuclease activities make MNase-seq unreliable for determining nucleosome occupancies, because cleavages within linker regions produce oligo- and mono-nucleosomes, whereas cleavages within nucleosomes de...
Autores principales: | Chereji, Răzvan V., Bryson, Terri D., Henikoff, Steven |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6743174/ https://www.ncbi.nlm.nih.gov/pubmed/31519205 http://dx.doi.org/10.1186/s13059-019-1815-z |
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