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Cell shape and intercellular adhesion regulate mitotic spindle orientation

Cell division orientation plays an essential role in tissue morphogenesis and cell fate decision. Recent studies showed that either cell shape or adhesion geometry can regulate the orientation of mitotic spindles and thereby the cell division orientation. However, how they together regulate the spin...

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Detalles Bibliográficos
Autores principales: Li, Jingchen, Cheng, Longcan, Jiang, Hongyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6743358/
https://www.ncbi.nlm.nih.gov/pubmed/31411941
http://dx.doi.org/10.1091/mbc.E19-04-0227
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author Li, Jingchen
Cheng, Longcan
Jiang, Hongyuan
author_facet Li, Jingchen
Cheng, Longcan
Jiang, Hongyuan
author_sort Li, Jingchen
collection PubMed
description Cell division orientation plays an essential role in tissue morphogenesis and cell fate decision. Recent studies showed that either cell shape or adhesion geometry can regulate the orientation of mitotic spindles and thereby the cell division orientation. However, how they together regulate the spindle orientation remains largely unclear. In this work, we use a general computational model to investigate the competitive mechanism of determining the spindle orientation between cell shape and intercellular adhesion in epithelial cells. We find the spindle orientation is dominated by the intercellular adhesion when the cell shape anisotropy is small, but dominated by the cell shape when the shape anisotropy is large. A strong adhesion and moderate adhesive size can ensure the planar division of epithelial cells with large apico-basal elongation. We also find the spindle orientation could be perpendicular to the adhesive region when only one side of the cell is adhered to an E-cadherin–coated matrix. But after the cell is compressed, the spindle orientation is governed by the cell shape and the spindle will be parallel to the adhesive region when the cell shape anisotropy is large. Finally, we demonstrate the competition between cell shape and tricellular junctions can also effectively regulate the spindle orientation.
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spelling pubmed-67433582019-11-16 Cell shape and intercellular adhesion regulate mitotic spindle orientation Li, Jingchen Cheng, Longcan Jiang, Hongyuan Mol Biol Cell Articles Cell division orientation plays an essential role in tissue morphogenesis and cell fate decision. Recent studies showed that either cell shape or adhesion geometry can regulate the orientation of mitotic spindles and thereby the cell division orientation. However, how they together regulate the spindle orientation remains largely unclear. In this work, we use a general computational model to investigate the competitive mechanism of determining the spindle orientation between cell shape and intercellular adhesion in epithelial cells. We find the spindle orientation is dominated by the intercellular adhesion when the cell shape anisotropy is small, but dominated by the cell shape when the shape anisotropy is large. A strong adhesion and moderate adhesive size can ensure the planar division of epithelial cells with large apico-basal elongation. We also find the spindle orientation could be perpendicular to the adhesive region when only one side of the cell is adhered to an E-cadherin–coated matrix. But after the cell is compressed, the spindle orientation is governed by the cell shape and the spindle will be parallel to the adhesive region when the cell shape anisotropy is large. Finally, we demonstrate the competition between cell shape and tricellular junctions can also effectively regulate the spindle orientation. The American Society for Cell Biology 2019-09-01 /pmc/articles/PMC6743358/ /pubmed/31411941 http://dx.doi.org/10.1091/mbc.E19-04-0227 Text en © 2019 Li et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Li, Jingchen
Cheng, Longcan
Jiang, Hongyuan
Cell shape and intercellular adhesion regulate mitotic spindle orientation
title Cell shape and intercellular adhesion regulate mitotic spindle orientation
title_full Cell shape and intercellular adhesion regulate mitotic spindle orientation
title_fullStr Cell shape and intercellular adhesion regulate mitotic spindle orientation
title_full_unstemmed Cell shape and intercellular adhesion regulate mitotic spindle orientation
title_short Cell shape and intercellular adhesion regulate mitotic spindle orientation
title_sort cell shape and intercellular adhesion regulate mitotic spindle orientation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6743358/
https://www.ncbi.nlm.nih.gov/pubmed/31411941
http://dx.doi.org/10.1091/mbc.E19-04-0227
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