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Spatial and temporal tools for building a human cell atlas

Improvements in the sensitivity, content, and throughput of microscopy, in the depth and throughput of single-cell sequencing approaches, and in computational and modeling tools for data integration have created a portfolio of methods for building spatiotemporal cell atlases. Challenges in this fast...

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Detalles Bibliográficos
Autores principales: Cool, Jonah, Conroy, Richard S., Hanlon, Sean E., Hughes, Shannon K., Roy, Ananda L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6743364/
https://www.ncbi.nlm.nih.gov/pubmed/31465255
http://dx.doi.org/10.1091/mbc.E18-10-0667
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author Cool, Jonah
Conroy, Richard S.
Hanlon, Sean E.
Hughes, Shannon K.
Roy, Ananda L.
author_facet Cool, Jonah
Conroy, Richard S.
Hanlon, Sean E.
Hughes, Shannon K.
Roy, Ananda L.
author_sort Cool, Jonah
collection PubMed
description Improvements in the sensitivity, content, and throughput of microscopy, in the depth and throughput of single-cell sequencing approaches, and in computational and modeling tools for data integration have created a portfolio of methods for building spatiotemporal cell atlases. Challenges in this fast-moving field include optimizing experimental conditions to allow a holistic view of tissues, extending molecular analysis across multiple timescales, and developing new tools for 1) managing large data sets, 2) extracting patterns and correlation from these data, and 3) integrating and visualizing data and derived results in an informative way. The utility of these tools and atlases for the broader scientific community will be accelerated through a commitment to findable, accessible, interoperable, and reusable data and tool sharing principles that can be facilitated through coordination and collaboration between programs working in this space.
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spelling pubmed-67433642019-11-16 Spatial and temporal tools for building a human cell atlas Cool, Jonah Conroy, Richard S. Hanlon, Sean E. Hughes, Shannon K. Roy, Ananda L. Mol Biol Cell Perspective Improvements in the sensitivity, content, and throughput of microscopy, in the depth and throughput of single-cell sequencing approaches, and in computational and modeling tools for data integration have created a portfolio of methods for building spatiotemporal cell atlases. Challenges in this fast-moving field include optimizing experimental conditions to allow a holistic view of tissues, extending molecular analysis across multiple timescales, and developing new tools for 1) managing large data sets, 2) extracting patterns and correlation from these data, and 3) integrating and visualizing data and derived results in an informative way. The utility of these tools and atlases for the broader scientific community will be accelerated through a commitment to findable, accessible, interoperable, and reusable data and tool sharing principles that can be facilitated through coordination and collaboration between programs working in this space. The American Society for Cell Biology 2019-09-01 /pmc/articles/PMC6743364/ /pubmed/31465255 http://dx.doi.org/10.1091/mbc.E18-10-0667 Text en © 2019 Cool et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Perspective
Cool, Jonah
Conroy, Richard S.
Hanlon, Sean E.
Hughes, Shannon K.
Roy, Ananda L.
Spatial and temporal tools for building a human cell atlas
title Spatial and temporal tools for building a human cell atlas
title_full Spatial and temporal tools for building a human cell atlas
title_fullStr Spatial and temporal tools for building a human cell atlas
title_full_unstemmed Spatial and temporal tools for building a human cell atlas
title_short Spatial and temporal tools for building a human cell atlas
title_sort spatial and temporal tools for building a human cell atlas
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6743364/
https://www.ncbi.nlm.nih.gov/pubmed/31465255
http://dx.doi.org/10.1091/mbc.E18-10-0667
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