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Tau tubulin kinase is required for spermatogenesis and development of motile cilia in planarian flatworms
Cilia are microtubule-based structures that protrude from the apical surface of cells to mediate motility, transport, intracellular signaling, and environmental sensing. Tau tubulin kinases (TTBKs) destabilize microtubules by phosphorylating microtubule-associated proteins (MAPs) of the MAP2/Tau fam...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6743461/ https://www.ncbi.nlm.nih.gov/pubmed/31141462 http://dx.doi.org/10.1091/mbc.E18-10-0663 |
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author | Magley, Robert Alan Rouhana, Labib |
author_facet | Magley, Robert Alan Rouhana, Labib |
author_sort | Magley, Robert Alan |
collection | PubMed |
description | Cilia are microtubule-based structures that protrude from the apical surface of cells to mediate motility, transport, intracellular signaling, and environmental sensing. Tau tubulin kinases (TTBKs) destabilize microtubules by phosphorylating microtubule-associated proteins (MAPs) of the MAP2/Tau family, but also contribute to the assembly of primary cilia during embryogenesis. Expression of TTBKs is enriched in testicular tissue, but their relevance to reproductive processes is unknown. We identified six TTBK homologues in the genome of the planarian Schmidtea mediterranea (Smed-TTBK-a, -b, -c, -d, -e, and -f), all of which are preferentially expressed in testes. Inhibition of TTBK paralogues by RNA interference (RNAi) revealed a specific requirement for Smed-TTBK-d in postmeiotic regulation of spermatogenesis. Disrupting expression of Smed-TTBK-d results in loss of spermatozoa, but not spermatids. In the soma, Smed-TTBK-d RNAi impaired the function of multiciliated epidermal cells in propelling planarian movement, as well as the osmoregulatory function of protonephridia. Decreased density and structural defects of motile cilia were observed in the epidermis of Smed-TTBK-d(RNAi) by phase contrast, immunofluorescence, and transmission electron microscopy. Altogether, these results demonstrate that members of the TTBK family of proteins are postmeiotic regulators of sperm development and also contribute to the formation of motile cilia in the soma. |
format | Online Article Text |
id | pubmed-6743461 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-67434612019-10-16 Tau tubulin kinase is required for spermatogenesis and development of motile cilia in planarian flatworms Magley, Robert Alan Rouhana, Labib Mol Biol Cell Articles Cilia are microtubule-based structures that protrude from the apical surface of cells to mediate motility, transport, intracellular signaling, and environmental sensing. Tau tubulin kinases (TTBKs) destabilize microtubules by phosphorylating microtubule-associated proteins (MAPs) of the MAP2/Tau family, but also contribute to the assembly of primary cilia during embryogenesis. Expression of TTBKs is enriched in testicular tissue, but their relevance to reproductive processes is unknown. We identified six TTBK homologues in the genome of the planarian Schmidtea mediterranea (Smed-TTBK-a, -b, -c, -d, -e, and -f), all of which are preferentially expressed in testes. Inhibition of TTBK paralogues by RNA interference (RNAi) revealed a specific requirement for Smed-TTBK-d in postmeiotic regulation of spermatogenesis. Disrupting expression of Smed-TTBK-d results in loss of spermatozoa, but not spermatids. In the soma, Smed-TTBK-d RNAi impaired the function of multiciliated epidermal cells in propelling planarian movement, as well as the osmoregulatory function of protonephridia. Decreased density and structural defects of motile cilia were observed in the epidermis of Smed-TTBK-d(RNAi) by phase contrast, immunofluorescence, and transmission electron microscopy. Altogether, these results demonstrate that members of the TTBK family of proteins are postmeiotic regulators of sperm development and also contribute to the formation of motile cilia in the soma. The American Society for Cell Biology 2019-08-01 /pmc/articles/PMC6743461/ /pubmed/31141462 http://dx.doi.org/10.1091/mbc.E18-10-0663 Text en © 2019 Magley and Rouhana. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License. |
spellingShingle | Articles Magley, Robert Alan Rouhana, Labib Tau tubulin kinase is required for spermatogenesis and development of motile cilia in planarian flatworms |
title | Tau tubulin kinase is required for spermatogenesis and development of motile cilia in planarian flatworms |
title_full | Tau tubulin kinase is required for spermatogenesis and development of motile cilia in planarian flatworms |
title_fullStr | Tau tubulin kinase is required for spermatogenesis and development of motile cilia in planarian flatworms |
title_full_unstemmed | Tau tubulin kinase is required for spermatogenesis and development of motile cilia in planarian flatworms |
title_short | Tau tubulin kinase is required for spermatogenesis and development of motile cilia in planarian flatworms |
title_sort | tau tubulin kinase is required for spermatogenesis and development of motile cilia in planarian flatworms |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6743461/ https://www.ncbi.nlm.nih.gov/pubmed/31141462 http://dx.doi.org/10.1091/mbc.E18-10-0663 |
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