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PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations

In this study, we describe the development of a PCR-free whole exome sequencing method. Using this method, 2 μg DNA was sufficient for library preparation for whole exome sequencing. Furthermore, the method is simple and makes use of a commercial kit, with additional step of concentrating the captur...

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Autores principales: Yamaguchi, Izumi, Watanabe, Takashi, Ohara, Osamu, Hasegawa, Yoshinori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6743761/
https://www.ncbi.nlm.nih.gov/pubmed/31518370
http://dx.doi.org/10.1371/journal.pone.0222562
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author Yamaguchi, Izumi
Watanabe, Takashi
Ohara, Osamu
Hasegawa, Yoshinori
author_facet Yamaguchi, Izumi
Watanabe, Takashi
Ohara, Osamu
Hasegawa, Yoshinori
author_sort Yamaguchi, Izumi
collection PubMed
description In this study, we describe the development of a PCR-free whole exome sequencing method. Using this method, 2 μg DNA was sufficient for library preparation for whole exome sequencing. Furthermore, the method is simple and makes use of a commercial kit, with additional step of concentrating the captured library by ethanol precipitation. The accuracy of the PCR-free method was found to be equivalent to that of unique molecular identifier-corrected analysis method, which is the commonly used method to detect rare mutations. Thus, the PCR-free whole exome sequencing method is cost-effective as well as efficient in detecting rare mutations.
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spelling pubmed-67437612019-09-20 PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations Yamaguchi, Izumi Watanabe, Takashi Ohara, Osamu Hasegawa, Yoshinori PLoS One Research Article In this study, we describe the development of a PCR-free whole exome sequencing method. Using this method, 2 μg DNA was sufficient for library preparation for whole exome sequencing. Furthermore, the method is simple and makes use of a commercial kit, with additional step of concentrating the captured library by ethanol precipitation. The accuracy of the PCR-free method was found to be equivalent to that of unique molecular identifier-corrected analysis method, which is the commonly used method to detect rare mutations. Thus, the PCR-free whole exome sequencing method is cost-effective as well as efficient in detecting rare mutations. Public Library of Science 2019-09-13 /pmc/articles/PMC6743761/ /pubmed/31518370 http://dx.doi.org/10.1371/journal.pone.0222562 Text en © 2019 Yamaguchi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yamaguchi, Izumi
Watanabe, Takashi
Ohara, Osamu
Hasegawa, Yoshinori
PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations
title PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations
title_full PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations
title_fullStr PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations
title_full_unstemmed PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations
title_short PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations
title_sort pcr-free whole exome sequencing: cost-effective and efficient in detecting rare mutations
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6743761/
https://www.ncbi.nlm.nih.gov/pubmed/31518370
http://dx.doi.org/10.1371/journal.pone.0222562
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