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Association of circulating exosomal miR-122 levels with BAT activity in healthy humans
Brown adipose tissue (BAT) plays an important role in body fat accumulation and the regulation of energy expenditure. Since the role of miRNAs in the pathogenesis of obesity and related metabolic diseases is contentious, we analyzed exosomal miRNAs in serum of healthy subjects with special reference...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6744505/ https://www.ncbi.nlm.nih.gov/pubmed/31519959 http://dx.doi.org/10.1038/s41598-019-49754-1 |
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author | Okamatsu-Ogura, Yuko Matsushita, Mami Bariuan, Jussiaea Valente Nagaya, Kazuki Tsubota, Ayumi Saito, Masayuki |
author_facet | Okamatsu-Ogura, Yuko Matsushita, Mami Bariuan, Jussiaea Valente Nagaya, Kazuki Tsubota, Ayumi Saito, Masayuki |
author_sort | Okamatsu-Ogura, Yuko |
collection | PubMed |
description | Brown adipose tissue (BAT) plays an important role in body fat accumulation and the regulation of energy expenditure. Since the role of miRNAs in the pathogenesis of obesity and related metabolic diseases is contentious, we analyzed exosomal miRNAs in serum of healthy subjects with special references to BAT activity and body fat level. Forty male volunteers aged 20–30 years were recruited. Their BAT activity was assessed by fluorodeoxyglucose positron emission tomography and computed tomography after 2 h of cold exposure and expressed as a maximal standardized uptake value (SUVmax). Exosomal miRNA levels was analyzed using microarray and real-time PCR analyses. The miR-122-5p level in the high BAT activity group (SUV ≧ 3) was 53% lower than in the low BAT activity group (SUVmax <3). Pearson’s correlation analysis revealed that the serum miR-122-5p level correlated negatively with BAT activity and the serum HDL-cholesterol, and it correlated positively with age, BMI, body fat mass, and total cholesterol and triglyceride serum levels. Multivariate regression analysis revealed that BAT activity was associated with the serum miR-122-5p level independently of the other parameters. These results reveal the serum exosomal miR-122-5p level is negatively associated with BAT activity independently of obesity. |
format | Online Article Text |
id | pubmed-6744505 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67445052019-09-27 Association of circulating exosomal miR-122 levels with BAT activity in healthy humans Okamatsu-Ogura, Yuko Matsushita, Mami Bariuan, Jussiaea Valente Nagaya, Kazuki Tsubota, Ayumi Saito, Masayuki Sci Rep Article Brown adipose tissue (BAT) plays an important role in body fat accumulation and the regulation of energy expenditure. Since the role of miRNAs in the pathogenesis of obesity and related metabolic diseases is contentious, we analyzed exosomal miRNAs in serum of healthy subjects with special references to BAT activity and body fat level. Forty male volunteers aged 20–30 years were recruited. Their BAT activity was assessed by fluorodeoxyglucose positron emission tomography and computed tomography after 2 h of cold exposure and expressed as a maximal standardized uptake value (SUVmax). Exosomal miRNA levels was analyzed using microarray and real-time PCR analyses. The miR-122-5p level in the high BAT activity group (SUV ≧ 3) was 53% lower than in the low BAT activity group (SUVmax <3). Pearson’s correlation analysis revealed that the serum miR-122-5p level correlated negatively with BAT activity and the serum HDL-cholesterol, and it correlated positively with age, BMI, body fat mass, and total cholesterol and triglyceride serum levels. Multivariate regression analysis revealed that BAT activity was associated with the serum miR-122-5p level independently of the other parameters. These results reveal the serum exosomal miR-122-5p level is negatively associated with BAT activity independently of obesity. Nature Publishing Group UK 2019-09-13 /pmc/articles/PMC6744505/ /pubmed/31519959 http://dx.doi.org/10.1038/s41598-019-49754-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Okamatsu-Ogura, Yuko Matsushita, Mami Bariuan, Jussiaea Valente Nagaya, Kazuki Tsubota, Ayumi Saito, Masayuki Association of circulating exosomal miR-122 levels with BAT activity in healthy humans |
title | Association of circulating exosomal miR-122 levels with BAT activity in healthy humans |
title_full | Association of circulating exosomal miR-122 levels with BAT activity in healthy humans |
title_fullStr | Association of circulating exosomal miR-122 levels with BAT activity in healthy humans |
title_full_unstemmed | Association of circulating exosomal miR-122 levels with BAT activity in healthy humans |
title_short | Association of circulating exosomal miR-122 levels with BAT activity in healthy humans |
title_sort | association of circulating exosomal mir-122 levels with bat activity in healthy humans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6744505/ https://www.ncbi.nlm.nih.gov/pubmed/31519959 http://dx.doi.org/10.1038/s41598-019-49754-1 |
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