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MiR-124 suppression in the prefrontal cortex reduces depression-like behavior in mice
Depression is a potentially life-threatening mental disorder with unknown etiology. Several microRNAs (miRNAs) have been shown to play critical roles in the etiology of depression. Here, we aim to elucidate the anti-depressive behavior of miR-124 suppression in prefrontal cortex (PFC). Quantitative...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6744582/ https://www.ncbi.nlm.nih.gov/pubmed/31431514 http://dx.doi.org/10.1042/BSR20190186 |
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author | Gu, Zhiwen Pan, Jiyang Chen, Liping |
author_facet | Gu, Zhiwen Pan, Jiyang Chen, Liping |
author_sort | Gu, Zhiwen |
collection | PubMed |
description | Depression is a potentially life-threatening mental disorder with unknown etiology. Several microRNAs (miRNAs) have been shown to play critical roles in the etiology of depression. Here, we aim to elucidate the anti-depressive behavior of miR-124 suppression in prefrontal cortex (PFC). Quantitative real-time PCR (RT-PCR) was used to evaluate the expression of miR-124 and SIRT1 in the PFC of a chronic unpredictable mild stress (CUMS) model. The PFC of C57BL/6J mice was bilaterally injected with lentiviral vectors (LV) for ectopic expression of SIRT1, miR-124, or miR-124 inhibitor (si-miR-124). The anti-depressive behavior was observed after injection of LV-SIRT1 or LV-si-miR-124 into the PFC, using behavior tests including latency to feed, food and water intake, sucrose preference test, and forced swimming test. MiR-124 overexpression and inhibition resulted in upregulation and down-regulation of SIRT1 and cyclic AMP responsive element binding protein 1 (CREB1), respectively. MiR-124 overexpression exacerbated depression-like behaviors and decreased SIRT1. Further, dual-luciferase assay confirmed that SIRT1 was a target of miR-124. Taken together, a potential molecular regulation of miR-124 on SIRT1 is revealed by our study and miR-124 suppression in PFC is a potential strategy to reduce depression-like behavior. |
format | Online Article Text |
id | pubmed-6744582 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67445822019-09-23 MiR-124 suppression in the prefrontal cortex reduces depression-like behavior in mice Gu, Zhiwen Pan, Jiyang Chen, Liping Biosci Rep Research Articles Depression is a potentially life-threatening mental disorder with unknown etiology. Several microRNAs (miRNAs) have been shown to play critical roles in the etiology of depression. Here, we aim to elucidate the anti-depressive behavior of miR-124 suppression in prefrontal cortex (PFC). Quantitative real-time PCR (RT-PCR) was used to evaluate the expression of miR-124 and SIRT1 in the PFC of a chronic unpredictable mild stress (CUMS) model. The PFC of C57BL/6J mice was bilaterally injected with lentiviral vectors (LV) for ectopic expression of SIRT1, miR-124, or miR-124 inhibitor (si-miR-124). The anti-depressive behavior was observed after injection of LV-SIRT1 or LV-si-miR-124 into the PFC, using behavior tests including latency to feed, food and water intake, sucrose preference test, and forced swimming test. MiR-124 overexpression and inhibition resulted in upregulation and down-regulation of SIRT1 and cyclic AMP responsive element binding protein 1 (CREB1), respectively. MiR-124 overexpression exacerbated depression-like behaviors and decreased SIRT1. Further, dual-luciferase assay confirmed that SIRT1 was a target of miR-124. Taken together, a potential molecular regulation of miR-124 on SIRT1 is revealed by our study and miR-124 suppression in PFC is a potential strategy to reduce depression-like behavior. Portland Press Ltd. 2019-09-13 /pmc/articles/PMC6744582/ /pubmed/31431514 http://dx.doi.org/10.1042/BSR20190186 Text en © 2019 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Gu, Zhiwen Pan, Jiyang Chen, Liping MiR-124 suppression in the prefrontal cortex reduces depression-like behavior in mice |
title | MiR-124 suppression in the prefrontal cortex reduces depression-like behavior in mice |
title_full | MiR-124 suppression in the prefrontal cortex reduces depression-like behavior in mice |
title_fullStr | MiR-124 suppression in the prefrontal cortex reduces depression-like behavior in mice |
title_full_unstemmed | MiR-124 suppression in the prefrontal cortex reduces depression-like behavior in mice |
title_short | MiR-124 suppression in the prefrontal cortex reduces depression-like behavior in mice |
title_sort | mir-124 suppression in the prefrontal cortex reduces depression-like behavior in mice |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6744582/ https://www.ncbi.nlm.nih.gov/pubmed/31431514 http://dx.doi.org/10.1042/BSR20190186 |
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