Cargando…

Genome-wide identification, characterization and expression pattern analysis of APYRASE family members in response to abiotic and biotic stresses in wheat

APYRASEs, which directly regulate intra- and extra-cellular ATP homeostasis, play a pivotal role in the regulation of various stress adaptations in mammals, bacteria and plants. In the present study, we identified and characterized wheat APYRASE family members at the genomic level in wheat. The resu...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Wenbo, Ni, Jun, Shah, Faheem Afzal, Ye, Kaiqin, Hu, Hao, Wang, Qiaojian, Wang, Dongdong, Yao, Yuanyuan, Huang, Shengwei, Hou, Jinyan, Liu, Chenghong, Wu, Lifang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6744936/
https://www.ncbi.nlm.nih.gov/pubmed/31565565
http://dx.doi.org/10.7717/peerj.7622
_version_ 1783451466640392192
author Liu, Wenbo
Ni, Jun
Shah, Faheem Afzal
Ye, Kaiqin
Hu, Hao
Wang, Qiaojian
Wang, Dongdong
Yao, Yuanyuan
Huang, Shengwei
Hou, Jinyan
Liu, Chenghong
Wu, Lifang
author_facet Liu, Wenbo
Ni, Jun
Shah, Faheem Afzal
Ye, Kaiqin
Hu, Hao
Wang, Qiaojian
Wang, Dongdong
Yao, Yuanyuan
Huang, Shengwei
Hou, Jinyan
Liu, Chenghong
Wu, Lifang
author_sort Liu, Wenbo
collection PubMed
description APYRASEs, which directly regulate intra- and extra-cellular ATP homeostasis, play a pivotal role in the regulation of various stress adaptations in mammals, bacteria and plants. In the present study, we identified and characterized wheat APYRASE family members at the genomic level in wheat. The results identified a total of nine APY homologs with conserved ACR domains. The sequence alignments, phylogenetic relations and conserved motifs of wheat APYs were bioinformatically analyzed. Although they share highly conserved secondary and tertiary structures, the wheat APYs could be mainly categorized into three groups, according to phylogenetic and structural analysis. Additionally, these APYs exhibited similar expression patterns in the root and shoot, among which TaAPY3-1, TaAPY3-3 and TaAPY3-4 had the highest expression levels. The time-course expression patterns of the eight APYs in response to biotic and abiotic stress in the wheat seedlings were also investigated. TaAPY3-2, TaAPY3-3, TaAPY3-4 and TaAPY6 exhibited strong sensitivity to all kinds of stresses in the leaves. Some APYs showed specific expression responses, such as TaAPY6 to heavy metal stress, and TaAPY7 to heat and salt stress. These results suggest that the stress-inducible APYs could have potential roles in the regulation of environmental stress adaptations. Moreover, the catalytic activity of TaAPY3-1 was further analyzed in the in vitro system. The results showed that TaAPY3-1 protein exhibited high catalytic activity in the degradation of ATP and ADP, but with low activity in degradation of TTP and GTP. It also has an extensive range of temperature adaptability, but preferred relatively acidic pH conditions. In this study, the genome-wide identification and characterization of APYs in wheat were suggested to be useful for further genetic modifications in the generation of high-stress-tolerant wheat cultivars.
format Online
Article
Text
id pubmed-6744936
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-67449362019-09-27 Genome-wide identification, characterization and expression pattern analysis of APYRASE family members in response to abiotic and biotic stresses in wheat Liu, Wenbo Ni, Jun Shah, Faheem Afzal Ye, Kaiqin Hu, Hao Wang, Qiaojian Wang, Dongdong Yao, Yuanyuan Huang, Shengwei Hou, Jinyan Liu, Chenghong Wu, Lifang PeerJ Genomics APYRASEs, which directly regulate intra- and extra-cellular ATP homeostasis, play a pivotal role in the regulation of various stress adaptations in mammals, bacteria and plants. In the present study, we identified and characterized wheat APYRASE family members at the genomic level in wheat. The results identified a total of nine APY homologs with conserved ACR domains. The sequence alignments, phylogenetic relations and conserved motifs of wheat APYs were bioinformatically analyzed. Although they share highly conserved secondary and tertiary structures, the wheat APYs could be mainly categorized into three groups, according to phylogenetic and structural analysis. Additionally, these APYs exhibited similar expression patterns in the root and shoot, among which TaAPY3-1, TaAPY3-3 and TaAPY3-4 had the highest expression levels. The time-course expression patterns of the eight APYs in response to biotic and abiotic stress in the wheat seedlings were also investigated. TaAPY3-2, TaAPY3-3, TaAPY3-4 and TaAPY6 exhibited strong sensitivity to all kinds of stresses in the leaves. Some APYs showed specific expression responses, such as TaAPY6 to heavy metal stress, and TaAPY7 to heat and salt stress. These results suggest that the stress-inducible APYs could have potential roles in the regulation of environmental stress adaptations. Moreover, the catalytic activity of TaAPY3-1 was further analyzed in the in vitro system. The results showed that TaAPY3-1 protein exhibited high catalytic activity in the degradation of ATP and ADP, but with low activity in degradation of TTP and GTP. It also has an extensive range of temperature adaptability, but preferred relatively acidic pH conditions. In this study, the genome-wide identification and characterization of APYs in wheat were suggested to be useful for further genetic modifications in the generation of high-stress-tolerant wheat cultivars. PeerJ Inc. 2019-09-11 /pmc/articles/PMC6744936/ /pubmed/31565565 http://dx.doi.org/10.7717/peerj.7622 Text en ©2019 Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Genomics
Liu, Wenbo
Ni, Jun
Shah, Faheem Afzal
Ye, Kaiqin
Hu, Hao
Wang, Qiaojian
Wang, Dongdong
Yao, Yuanyuan
Huang, Shengwei
Hou, Jinyan
Liu, Chenghong
Wu, Lifang
Genome-wide identification, characterization and expression pattern analysis of APYRASE family members in response to abiotic and biotic stresses in wheat
title Genome-wide identification, characterization and expression pattern analysis of APYRASE family members in response to abiotic and biotic stresses in wheat
title_full Genome-wide identification, characterization and expression pattern analysis of APYRASE family members in response to abiotic and biotic stresses in wheat
title_fullStr Genome-wide identification, characterization and expression pattern analysis of APYRASE family members in response to abiotic and biotic stresses in wheat
title_full_unstemmed Genome-wide identification, characterization and expression pattern analysis of APYRASE family members in response to abiotic and biotic stresses in wheat
title_short Genome-wide identification, characterization and expression pattern analysis of APYRASE family members in response to abiotic and biotic stresses in wheat
title_sort genome-wide identification, characterization and expression pattern analysis of apyrase family members in response to abiotic and biotic stresses in wheat
topic Genomics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6744936/
https://www.ncbi.nlm.nih.gov/pubmed/31565565
http://dx.doi.org/10.7717/peerj.7622
work_keys_str_mv AT liuwenbo genomewideidentificationcharacterizationandexpressionpatternanalysisofapyrasefamilymembersinresponsetoabioticandbioticstressesinwheat
AT nijun genomewideidentificationcharacterizationandexpressionpatternanalysisofapyrasefamilymembersinresponsetoabioticandbioticstressesinwheat
AT shahfaheemafzal genomewideidentificationcharacterizationandexpressionpatternanalysisofapyrasefamilymembersinresponsetoabioticandbioticstressesinwheat
AT yekaiqin genomewideidentificationcharacterizationandexpressionpatternanalysisofapyrasefamilymembersinresponsetoabioticandbioticstressesinwheat
AT huhao genomewideidentificationcharacterizationandexpressionpatternanalysisofapyrasefamilymembersinresponsetoabioticandbioticstressesinwheat
AT wangqiaojian genomewideidentificationcharacterizationandexpressionpatternanalysisofapyrasefamilymembersinresponsetoabioticandbioticstressesinwheat
AT wangdongdong genomewideidentificationcharacterizationandexpressionpatternanalysisofapyrasefamilymembersinresponsetoabioticandbioticstressesinwheat
AT yaoyuanyuan genomewideidentificationcharacterizationandexpressionpatternanalysisofapyrasefamilymembersinresponsetoabioticandbioticstressesinwheat
AT huangshengwei genomewideidentificationcharacterizationandexpressionpatternanalysisofapyrasefamilymembersinresponsetoabioticandbioticstressesinwheat
AT houjinyan genomewideidentificationcharacterizationandexpressionpatternanalysisofapyrasefamilymembersinresponsetoabioticandbioticstressesinwheat
AT liuchenghong genomewideidentificationcharacterizationandexpressionpatternanalysisofapyrasefamilymembersinresponsetoabioticandbioticstressesinwheat
AT wulifang genomewideidentificationcharacterizationandexpressionpatternanalysisofapyrasefamilymembersinresponsetoabioticandbioticstressesinwheat