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Tissue P16 is Associated with Smoking Status among Indonesian Nasopharyngeal Carcinoma Subjects

BACKGROUND: Nasopharyngeal carcinoma (NPC) is a malignancy with high incidence in Southern China and South-East Asia. NPC incidence among males in Indonesia is estimated around 8.3/100,000 populations. Tobacco smoking is a common risk factor for cancer, including NPC. P16 is one of the key proteins...

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Autores principales: Wahyuningsih, Laila, Dwianingsih, Ery Kus, Risanti, Erika Diana, Tirtoprodjo, Prijono, Rinonce, Hanggoro Tri, Hakim, Fikar Arsyad, Herdini, Camelia, Fachiroh, Jajah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2019
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6745211/
https://www.ncbi.nlm.nih.gov/pubmed/31350975
http://dx.doi.org/10.31557/APJCP.2019.20.7.2125
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author Wahyuningsih, Laila
Dwianingsih, Ery Kus
Risanti, Erika Diana
Tirtoprodjo, Prijono
Rinonce, Hanggoro Tri
Hakim, Fikar Arsyad
Herdini, Camelia
Fachiroh, Jajah
author_facet Wahyuningsih, Laila
Dwianingsih, Ery Kus
Risanti, Erika Diana
Tirtoprodjo, Prijono
Rinonce, Hanggoro Tri
Hakim, Fikar Arsyad
Herdini, Camelia
Fachiroh, Jajah
author_sort Wahyuningsih, Laila
collection PubMed
description BACKGROUND: Nasopharyngeal carcinoma (NPC) is a malignancy with high incidence in Southern China and South-East Asia. NPC incidence among males in Indonesia is estimated around 8.3/100,000 populations. Tobacco smoking is a common risk factor for cancer, including NPC. P16 is one of the key proteins related to the activation of apoptotic pathways, that commonly change during carcinogenesis. Carcinogenesis is often related to environmental exposure, including tobacco smoke. OBJECTIVE: To analyze the association between P16 protein and smoking status among NPC subjects in Indonesia. METHODS: Forty formalin fixed-paraffin embedded NPC tissue samples of known smoking status (20 smokers, 20 non-smokers) were collected from the Department of Anatomical Pathology, Dr. Sardjito Hospital, Yogyakarta. P16 was detected by immunohistochemistry staining. German semi-quantitative scoring system was applied to the P16 staining. Expression index with the score of 0 to 3 was classified as negative staining, meanwhile 4 to 12 was classified as positive staining. The association between P16 (score) and smoking status among NPC patients was analyzed using Fischer exact test. One-sided p ≤ 0.05 was considered as statistically significant. RESULTS: All samples were Javanese males, with age range 25-76 years old. P16 positive staining among smokers was 5% (1/20), while among non-smokers was 40% (8/20). P16 among smokers was significantly lower than non-smokers patients (p=0.010). No difference was found between quantity of smoke and P16 score. CONCLUSION : A significant association between P16 and smoking status in Indonesian NPC patients has been revealed. The result of this study may be used to improve prevention and management of NPC cases related to smoking habit in Indonesia.
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spelling pubmed-67452112019-10-03 Tissue P16 is Associated with Smoking Status among Indonesian Nasopharyngeal Carcinoma Subjects Wahyuningsih, Laila Dwianingsih, Ery Kus Risanti, Erika Diana Tirtoprodjo, Prijono Rinonce, Hanggoro Tri Hakim, Fikar Arsyad Herdini, Camelia Fachiroh, Jajah Asian Pac J Cancer Prev Research Article BACKGROUND: Nasopharyngeal carcinoma (NPC) is a malignancy with high incidence in Southern China and South-East Asia. NPC incidence among males in Indonesia is estimated around 8.3/100,000 populations. Tobacco smoking is a common risk factor for cancer, including NPC. P16 is one of the key proteins related to the activation of apoptotic pathways, that commonly change during carcinogenesis. Carcinogenesis is often related to environmental exposure, including tobacco smoke. OBJECTIVE: To analyze the association between P16 protein and smoking status among NPC subjects in Indonesia. METHODS: Forty formalin fixed-paraffin embedded NPC tissue samples of known smoking status (20 smokers, 20 non-smokers) were collected from the Department of Anatomical Pathology, Dr. Sardjito Hospital, Yogyakarta. P16 was detected by immunohistochemistry staining. German semi-quantitative scoring system was applied to the P16 staining. Expression index with the score of 0 to 3 was classified as negative staining, meanwhile 4 to 12 was classified as positive staining. The association between P16 (score) and smoking status among NPC patients was analyzed using Fischer exact test. One-sided p ≤ 0.05 was considered as statistically significant. RESULTS: All samples were Javanese males, with age range 25-76 years old. P16 positive staining among smokers was 5% (1/20), while among non-smokers was 40% (8/20). P16 among smokers was significantly lower than non-smokers patients (p=0.010). No difference was found between quantity of smoke and P16 score. CONCLUSION : A significant association between P16 and smoking status in Indonesian NPC patients has been revealed. The result of this study may be used to improve prevention and management of NPC cases related to smoking habit in Indonesia. West Asia Organization for Cancer Prevention 2019 /pmc/articles/PMC6745211/ /pubmed/31350975 http://dx.doi.org/10.31557/APJCP.2019.20.7.2125 Text en © Asian Pacific Journal of Cancer Prevention This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wahyuningsih, Laila
Dwianingsih, Ery Kus
Risanti, Erika Diana
Tirtoprodjo, Prijono
Rinonce, Hanggoro Tri
Hakim, Fikar Arsyad
Herdini, Camelia
Fachiroh, Jajah
Tissue P16 is Associated with Smoking Status among Indonesian Nasopharyngeal Carcinoma Subjects
title Tissue P16 is Associated with Smoking Status among Indonesian Nasopharyngeal Carcinoma Subjects
title_full Tissue P16 is Associated with Smoking Status among Indonesian Nasopharyngeal Carcinoma Subjects
title_fullStr Tissue P16 is Associated with Smoking Status among Indonesian Nasopharyngeal Carcinoma Subjects
title_full_unstemmed Tissue P16 is Associated with Smoking Status among Indonesian Nasopharyngeal Carcinoma Subjects
title_short Tissue P16 is Associated with Smoking Status among Indonesian Nasopharyngeal Carcinoma Subjects
title_sort tissue p16 is associated with smoking status among indonesian nasopharyngeal carcinoma subjects
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6745211/
https://www.ncbi.nlm.nih.gov/pubmed/31350975
http://dx.doi.org/10.31557/APJCP.2019.20.7.2125
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