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Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice
BACKGROUND: The molecular pathways of how endocrine disruptors affect bone mineral density (BMD) and bone remodeling are still unclear. The purpose of this experimental study is to determine the effects of di(2-ethylhexyl)phthalate (DEHP) on bone metabolism in ovariectomized mice. METHODS: Twenty-si...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Korean Society for Bone and Mineral Research
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746662/ https://www.ncbi.nlm.nih.gov/pubmed/31555614 http://dx.doi.org/10.11005/jbm.2019.26.3.169 |
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author | Choi, Jeong In Cho, Hyun Hee |
author_facet | Choi, Jeong In Cho, Hyun Hee |
author_sort | Choi, Jeong In |
collection | PubMed |
description | BACKGROUND: The molecular pathways of how endocrine disruptors affect bone mineral density (BMD) and bone remodeling are still unclear. The purpose of this experimental study is to determine the effects of di(2-ethylhexyl)phthalate (DEHP) on bone metabolism in ovariectomized mice. METHODS: Twenty-six-month-old female CD-1 mice were divided into 4 groups: control, low-dose DEHP, high-dose DEHP, and estrogen groups (n=5, each group). All mice were subjected to ovariectomy for the induction of artificial menopause and then exposed to corn oil, DEHP, and estrogen for 2 months. Micro-computed tomography (Micro-CT) of the bone and analysis of blood samples for bone markers were performed to observe the changes in bone metabolism. RESULTS: Osteocalcin level was decreased in the control, low-dose and high-dose DEHP group, the reduction width was greater in the high-dose DEHP group (−0.219 ng/mL) than control group (−0.077 ng/mL, P<0.05). C-terminal telopeptide of type I collagen level was increased in the control, low-dose and high-dose DEHP group, the increase range of low-dose DEHP group (0.329 ng/mL) showed greater than control group (0.093 ng/mL, P<0.05). Micro-CT analysis revealed that the BMD was significantly lower in the high-dose DEHP group (19.8×10(−2) g/cm(3)) than control group (27.2×10(−2) g/cm(3), P<0.05). The structure model index was significantly higher in the high-dose DEHP group (2.737) than low-dose DEHP group (2.648) and estrogen group (2.63, P<0.05). It means the progression of osteoporosis in the high-dose DEHP group. CONCLUSIONS: These results confirm the negative effects of DEHP on bone health in ovariectomized mice. Further continuous studies on genetic pathways and other endocrine disruptors will be necessary to validate these findings. |
format | Online Article Text |
id | pubmed-6746662 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Korean Society for Bone and Mineral Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-67466622019-09-25 Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice Choi, Jeong In Cho, Hyun Hee J Bone Metab Original Article BACKGROUND: The molecular pathways of how endocrine disruptors affect bone mineral density (BMD) and bone remodeling are still unclear. The purpose of this experimental study is to determine the effects of di(2-ethylhexyl)phthalate (DEHP) on bone metabolism in ovariectomized mice. METHODS: Twenty-six-month-old female CD-1 mice were divided into 4 groups: control, low-dose DEHP, high-dose DEHP, and estrogen groups (n=5, each group). All mice were subjected to ovariectomy for the induction of artificial menopause and then exposed to corn oil, DEHP, and estrogen for 2 months. Micro-computed tomography (Micro-CT) of the bone and analysis of blood samples for bone markers were performed to observe the changes in bone metabolism. RESULTS: Osteocalcin level was decreased in the control, low-dose and high-dose DEHP group, the reduction width was greater in the high-dose DEHP group (−0.219 ng/mL) than control group (−0.077 ng/mL, P<0.05). C-terminal telopeptide of type I collagen level was increased in the control, low-dose and high-dose DEHP group, the increase range of low-dose DEHP group (0.329 ng/mL) showed greater than control group (0.093 ng/mL, P<0.05). Micro-CT analysis revealed that the BMD was significantly lower in the high-dose DEHP group (19.8×10(−2) g/cm(3)) than control group (27.2×10(−2) g/cm(3), P<0.05). The structure model index was significantly higher in the high-dose DEHP group (2.737) than low-dose DEHP group (2.648) and estrogen group (2.63, P<0.05). It means the progression of osteoporosis in the high-dose DEHP group. CONCLUSIONS: These results confirm the negative effects of DEHP on bone health in ovariectomized mice. Further continuous studies on genetic pathways and other endocrine disruptors will be necessary to validate these findings. The Korean Society for Bone and Mineral Research 2019-08 2019-08-31 /pmc/articles/PMC6746662/ /pubmed/31555614 http://dx.doi.org/10.11005/jbm.2019.26.3.169 Text en Copyright © 2019 The Korean Society for Bone and Mineral Research http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Choi, Jeong In Cho, Hyun Hee Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice |
title | Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice |
title_full | Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice |
title_fullStr | Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice |
title_full_unstemmed | Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice |
title_short | Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice |
title_sort | effects of di(2-ethylhexyl)phthalate on bone metabolism in ovariectomized mice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746662/ https://www.ncbi.nlm.nih.gov/pubmed/31555614 http://dx.doi.org/10.11005/jbm.2019.26.3.169 |
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