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Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice

BACKGROUND: The molecular pathways of how endocrine disruptors affect bone mineral density (BMD) and bone remodeling are still unclear. The purpose of this experimental study is to determine the effects of di(2-ethylhexyl)phthalate (DEHP) on bone metabolism in ovariectomized mice. METHODS: Twenty-si...

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Autores principales: Choi, Jeong In, Cho, Hyun Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Bone and Mineral Research 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746662/
https://www.ncbi.nlm.nih.gov/pubmed/31555614
http://dx.doi.org/10.11005/jbm.2019.26.3.169
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author Choi, Jeong In
Cho, Hyun Hee
author_facet Choi, Jeong In
Cho, Hyun Hee
author_sort Choi, Jeong In
collection PubMed
description BACKGROUND: The molecular pathways of how endocrine disruptors affect bone mineral density (BMD) and bone remodeling are still unclear. The purpose of this experimental study is to determine the effects of di(2-ethylhexyl)phthalate (DEHP) on bone metabolism in ovariectomized mice. METHODS: Twenty-six-month-old female CD-1 mice were divided into 4 groups: control, low-dose DEHP, high-dose DEHP, and estrogen groups (n=5, each group). All mice were subjected to ovariectomy for the induction of artificial menopause and then exposed to corn oil, DEHP, and estrogen for 2 months. Micro-computed tomography (Micro-CT) of the bone and analysis of blood samples for bone markers were performed to observe the changes in bone metabolism. RESULTS: Osteocalcin level was decreased in the control, low-dose and high-dose DEHP group, the reduction width was greater in the high-dose DEHP group (−0.219 ng/mL) than control group (−0.077 ng/mL, P<0.05). C-terminal telopeptide of type I collagen level was increased in the control, low-dose and high-dose DEHP group, the increase range of low-dose DEHP group (0.329 ng/mL) showed greater than control group (0.093 ng/mL, P<0.05). Micro-CT analysis revealed that the BMD was significantly lower in the high-dose DEHP group (19.8×10(−2) g/cm(3)) than control group (27.2×10(−2) g/cm(3), P<0.05). The structure model index was significantly higher in the high-dose DEHP group (2.737) than low-dose DEHP group (2.648) and estrogen group (2.63, P<0.05). It means the progression of osteoporosis in the high-dose DEHP group. CONCLUSIONS: These results confirm the negative effects of DEHP on bone health in ovariectomized mice. Further continuous studies on genetic pathways and other endocrine disruptors will be necessary to validate these findings.
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spelling pubmed-67466622019-09-25 Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice Choi, Jeong In Cho, Hyun Hee J Bone Metab Original Article BACKGROUND: The molecular pathways of how endocrine disruptors affect bone mineral density (BMD) and bone remodeling are still unclear. The purpose of this experimental study is to determine the effects of di(2-ethylhexyl)phthalate (DEHP) on bone metabolism in ovariectomized mice. METHODS: Twenty-six-month-old female CD-1 mice were divided into 4 groups: control, low-dose DEHP, high-dose DEHP, and estrogen groups (n=5, each group). All mice were subjected to ovariectomy for the induction of artificial menopause and then exposed to corn oil, DEHP, and estrogen for 2 months. Micro-computed tomography (Micro-CT) of the bone and analysis of blood samples for bone markers were performed to observe the changes in bone metabolism. RESULTS: Osteocalcin level was decreased in the control, low-dose and high-dose DEHP group, the reduction width was greater in the high-dose DEHP group (−0.219 ng/mL) than control group (−0.077 ng/mL, P<0.05). C-terminal telopeptide of type I collagen level was increased in the control, low-dose and high-dose DEHP group, the increase range of low-dose DEHP group (0.329 ng/mL) showed greater than control group (0.093 ng/mL, P<0.05). Micro-CT analysis revealed that the BMD was significantly lower in the high-dose DEHP group (19.8×10(−2) g/cm(3)) than control group (27.2×10(−2) g/cm(3), P<0.05). The structure model index was significantly higher in the high-dose DEHP group (2.737) than low-dose DEHP group (2.648) and estrogen group (2.63, P<0.05). It means the progression of osteoporosis in the high-dose DEHP group. CONCLUSIONS: These results confirm the negative effects of DEHP on bone health in ovariectomized mice. Further continuous studies on genetic pathways and other endocrine disruptors will be necessary to validate these findings. The Korean Society for Bone and Mineral Research 2019-08 2019-08-31 /pmc/articles/PMC6746662/ /pubmed/31555614 http://dx.doi.org/10.11005/jbm.2019.26.3.169 Text en Copyright © 2019 The Korean Society for Bone and Mineral Research http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Choi, Jeong In
Cho, Hyun Hee
Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice
title Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice
title_full Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice
title_fullStr Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice
title_full_unstemmed Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice
title_short Effects of Di(2-ethylhexyl)phthalate on Bone Metabolism in Ovariectomized Mice
title_sort effects of di(2-ethylhexyl)phthalate on bone metabolism in ovariectomized mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746662/
https://www.ncbi.nlm.nih.gov/pubmed/31555614
http://dx.doi.org/10.11005/jbm.2019.26.3.169
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