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Mixing brain cerebrosides with brain ceramides, cholesterol and phospholipids
The properties of bilayers composed of pure brain cerebroside (bCrb) or of binary mixtures of bCrb with brain ceramide, cholesterol, egg phosphatidylcholine or brain sphingomyelin have been studied using a combination of physical techniques. Pure bCrb exhibits a rather narrow gel-fluid transition ce...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746848/ https://www.ncbi.nlm.nih.gov/pubmed/31527655 http://dx.doi.org/10.1038/s41598-019-50020-7 |
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author | González-Ramírez, Emilio J. Goñi, Félix M. Alonso, Alicia |
author_facet | González-Ramírez, Emilio J. Goñi, Félix M. Alonso, Alicia |
author_sort | González-Ramírez, Emilio J. |
collection | PubMed |
description | The properties of bilayers composed of pure brain cerebroside (bCrb) or of binary mixtures of bCrb with brain ceramide, cholesterol, egg phosphatidylcholine or brain sphingomyelin have been studied using a combination of physical techniques. Pure bCrb exhibits a rather narrow gel-fluid transition centred at ≈65 °C, with a half-width at half-height T(1/2) ≈ 3 °C. bCrb mixes well with both fluid and gel phospholipids and ceramide, and it rigidifies bilayers of egg phosphatidylcholine or brain sphingomyelin when the latter are in the fluid state. Cholesterol markedly widens the bCrb gel-fluid transition, while decreasing the associated transition enthalpy, in the manner of cholesterol mixtures with saturated phosphatidylcholines, or sphingomyelins. Laurdan and DPH fluorescence indicate the formation of fluid ordered phases in the bCrb:cholesterol mixtures. Macroscopic phase separation of more and less fluid domains is observed in giant unilamellar vesicles consisting of bCrb:egg phosphatidylcholine or bCrb:sphingomyelin. Crb capacity to induce bilayer permeabilization or transbilayer (flip-flop) lipid motion is much lower than those of ceramides. The mixtures explored here contained mostly bCrb concentrations >50 mol%, mimicking the situation of cell membranes in Gaucher’s disease, or of the Crb-enriched microdomains proposed to exist in healthy cell plasma membranes. |
format | Online Article Text |
id | pubmed-6746848 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67468482019-09-27 Mixing brain cerebrosides with brain ceramides, cholesterol and phospholipids González-Ramírez, Emilio J. Goñi, Félix M. Alonso, Alicia Sci Rep Article The properties of bilayers composed of pure brain cerebroside (bCrb) or of binary mixtures of bCrb with brain ceramide, cholesterol, egg phosphatidylcholine or brain sphingomyelin have been studied using a combination of physical techniques. Pure bCrb exhibits a rather narrow gel-fluid transition centred at ≈65 °C, with a half-width at half-height T(1/2) ≈ 3 °C. bCrb mixes well with both fluid and gel phospholipids and ceramide, and it rigidifies bilayers of egg phosphatidylcholine or brain sphingomyelin when the latter are in the fluid state. Cholesterol markedly widens the bCrb gel-fluid transition, while decreasing the associated transition enthalpy, in the manner of cholesterol mixtures with saturated phosphatidylcholines, or sphingomyelins. Laurdan and DPH fluorescence indicate the formation of fluid ordered phases in the bCrb:cholesterol mixtures. Macroscopic phase separation of more and less fluid domains is observed in giant unilamellar vesicles consisting of bCrb:egg phosphatidylcholine or bCrb:sphingomyelin. Crb capacity to induce bilayer permeabilization or transbilayer (flip-flop) lipid motion is much lower than those of ceramides. The mixtures explored here contained mostly bCrb concentrations >50 mol%, mimicking the situation of cell membranes in Gaucher’s disease, or of the Crb-enriched microdomains proposed to exist in healthy cell plasma membranes. Nature Publishing Group UK 2019-09-16 /pmc/articles/PMC6746848/ /pubmed/31527655 http://dx.doi.org/10.1038/s41598-019-50020-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article González-Ramírez, Emilio J. Goñi, Félix M. Alonso, Alicia Mixing brain cerebrosides with brain ceramides, cholesterol and phospholipids |
title | Mixing brain cerebrosides with brain ceramides, cholesterol and phospholipids |
title_full | Mixing brain cerebrosides with brain ceramides, cholesterol and phospholipids |
title_fullStr | Mixing brain cerebrosides with brain ceramides, cholesterol and phospholipids |
title_full_unstemmed | Mixing brain cerebrosides with brain ceramides, cholesterol and phospholipids |
title_short | Mixing brain cerebrosides with brain ceramides, cholesterol and phospholipids |
title_sort | mixing brain cerebrosides with brain ceramides, cholesterol and phospholipids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746848/ https://www.ncbi.nlm.nih.gov/pubmed/31527655 http://dx.doi.org/10.1038/s41598-019-50020-7 |
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