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Transcriptome analysis of fowl adenovirus serotype 4 infection in chickens

Fowl adenovirus serotype 4 (FAdV-4) is a causative agent of inclusion body hepatitis and hydropericardium–hepatitis syndrome. These diseases cause considerable economic losses in the global poultry industry and are significant stressors for infected chickens. However, the molecular mechanisms of FAd...

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Autores principales: Ren, Guangcai, Wang, Han, Huang, Miaorong, Yan, Yuanyuan, Liu, Fan, Chen, Ruiai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746880/
https://www.ncbi.nlm.nih.gov/pubmed/31264023
http://dx.doi.org/10.1007/s11262-019-01676-w
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author Ren, Guangcai
Wang, Han
Huang, Miaorong
Yan, Yuanyuan
Liu, Fan
Chen, Ruiai
author_facet Ren, Guangcai
Wang, Han
Huang, Miaorong
Yan, Yuanyuan
Liu, Fan
Chen, Ruiai
author_sort Ren, Guangcai
collection PubMed
description Fowl adenovirus serotype 4 (FAdV-4) is a causative agent of inclusion body hepatitis and hydropericardium–hepatitis syndrome. These diseases cause considerable economic losses in the global poultry industry and are significant stressors for infected chickens. However, the molecular mechanisms of FAdV-4 pathogenesis are poorly understood. In the present study, we identified differentially expressed genes from the livers of FAdV-4-infected chickens using RNA-seq at 7, 14 and 21 days after FAdV-4 infection. We identified 2395 differentially expressed genes at the three time points. These genes were enriched in variety of biological processes and pathways including PPAR and Notch signaling, cytokine–cytokine receptor interactions and Toll-like receptor signaling pathways. The transcriptional data were validated by quantitative real-time PCR. Our results will assist in the understanding of the molecular pathogenesis of FAdV-4 infection and for developing novel antiviral therapies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11262-019-01676-w) contains supplementary material, which is available to authorized users.
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spelling pubmed-67468802019-09-30 Transcriptome analysis of fowl adenovirus serotype 4 infection in chickens Ren, Guangcai Wang, Han Huang, Miaorong Yan, Yuanyuan Liu, Fan Chen, Ruiai Virus Genes Original Paper Fowl adenovirus serotype 4 (FAdV-4) is a causative agent of inclusion body hepatitis and hydropericardium–hepatitis syndrome. These diseases cause considerable economic losses in the global poultry industry and are significant stressors for infected chickens. However, the molecular mechanisms of FAdV-4 pathogenesis are poorly understood. In the present study, we identified differentially expressed genes from the livers of FAdV-4-infected chickens using RNA-seq at 7, 14 and 21 days after FAdV-4 infection. We identified 2395 differentially expressed genes at the three time points. These genes were enriched in variety of biological processes and pathways including PPAR and Notch signaling, cytokine–cytokine receptor interactions and Toll-like receptor signaling pathways. The transcriptional data were validated by quantitative real-time PCR. Our results will assist in the understanding of the molecular pathogenesis of FAdV-4 infection and for developing novel antiviral therapies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11262-019-01676-w) contains supplementary material, which is available to authorized users. Springer US 2019-07-01 2019 /pmc/articles/PMC6746880/ /pubmed/31264023 http://dx.doi.org/10.1007/s11262-019-01676-w Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Paper
Ren, Guangcai
Wang, Han
Huang, Miaorong
Yan, Yuanyuan
Liu, Fan
Chen, Ruiai
Transcriptome analysis of fowl adenovirus serotype 4 infection in chickens
title Transcriptome analysis of fowl adenovirus serotype 4 infection in chickens
title_full Transcriptome analysis of fowl adenovirus serotype 4 infection in chickens
title_fullStr Transcriptome analysis of fowl adenovirus serotype 4 infection in chickens
title_full_unstemmed Transcriptome analysis of fowl adenovirus serotype 4 infection in chickens
title_short Transcriptome analysis of fowl adenovirus serotype 4 infection in chickens
title_sort transcriptome analysis of fowl adenovirus serotype 4 infection in chickens
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6746880/
https://www.ncbi.nlm.nih.gov/pubmed/31264023
http://dx.doi.org/10.1007/s11262-019-01676-w
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