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Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification

Genetically encoded biosensors based on fluorescent proteins (FPs) are a reliable tool for studying the various biological processes in living systems. The circular permutation of single FPs led to the development of an extensive class of biosensors that allow the monitoring of many intracellular ev...

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Detalles Bibliográficos
Autores principales: Kostyuk, Alexander I., Demidovich, Aleksandra D., Kotova, Daria A., Belousov, Vsevolod V., Bilan, Dmitry S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6747460/
https://www.ncbi.nlm.nih.gov/pubmed/31461959
http://dx.doi.org/10.3390/ijms20174200
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author Kostyuk, Alexander I.
Demidovich, Aleksandra D.
Kotova, Daria A.
Belousov, Vsevolod V.
Bilan, Dmitry S.
author_facet Kostyuk, Alexander I.
Demidovich, Aleksandra D.
Kotova, Daria A.
Belousov, Vsevolod V.
Bilan, Dmitry S.
author_sort Kostyuk, Alexander I.
collection PubMed
description Genetically encoded biosensors based on fluorescent proteins (FPs) are a reliable tool for studying the various biological processes in living systems. The circular permutation of single FPs led to the development of an extensive class of biosensors that allow the monitoring of many intracellular events. In circularly permuted FPs (cpFPs), the original N- and C-termini are fused using a peptide linker, while new termini are formed near the chromophore. Such a structure imparts greater mobility to the FP than that of the native variant, allowing greater lability of the spectral characteristics. One of the common principles of creating genetically encoded biosensors is based on the integration of a cpFP into a flexible region of a sensory domain or between two interacting domains, which are selected according to certain characteristics. Conformational rearrangements of the sensory domain associated with ligand interaction or changes in the cellular parameter are transferred to the cpFP, changing the chromophore environment. In this review, we highlight the basic principles of such sensors, the history of their creation, and a complete classification of the available biosensors.
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spelling pubmed-67474602019-09-27 Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification Kostyuk, Alexander I. Demidovich, Aleksandra D. Kotova, Daria A. Belousov, Vsevolod V. Bilan, Dmitry S. Int J Mol Sci Review Genetically encoded biosensors based on fluorescent proteins (FPs) are a reliable tool for studying the various biological processes in living systems. The circular permutation of single FPs led to the development of an extensive class of biosensors that allow the monitoring of many intracellular events. In circularly permuted FPs (cpFPs), the original N- and C-termini are fused using a peptide linker, while new termini are formed near the chromophore. Such a structure imparts greater mobility to the FP than that of the native variant, allowing greater lability of the spectral characteristics. One of the common principles of creating genetically encoded biosensors is based on the integration of a cpFP into a flexible region of a sensory domain or between two interacting domains, which are selected according to certain characteristics. Conformational rearrangements of the sensory domain associated with ligand interaction or changes in the cellular parameter are transferred to the cpFP, changing the chromophore environment. In this review, we highlight the basic principles of such sensors, the history of their creation, and a complete classification of the available biosensors. MDPI 2019-08-27 /pmc/articles/PMC6747460/ /pubmed/31461959 http://dx.doi.org/10.3390/ijms20174200 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Kostyuk, Alexander I.
Demidovich, Aleksandra D.
Kotova, Daria A.
Belousov, Vsevolod V.
Bilan, Dmitry S.
Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification
title Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification
title_full Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification
title_fullStr Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification
title_full_unstemmed Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification
title_short Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification
title_sort circularly permuted fluorescent protein-based indicators: history, principles, and classification
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6747460/
https://www.ncbi.nlm.nih.gov/pubmed/31461959
http://dx.doi.org/10.3390/ijms20174200
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