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Antimicrobial Activity of Protamine-Loaded Calcium Phosphates against Oral Bacteria

Protamine is an antimicrobial peptide extracted from fish. In this study, we loaded protamine onto dicalcium phosphate anhydride (DCPA), a dental material. Protamine was loaded by stirring DCPA into a protamine solution. To explore the antimicrobial activity of the materials, we cultivated Streptoco...

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Autores principales: Fujiki, Masashi, Abe, Kodai, Hayakawa, Tohru, Yamamoto, Takatsugu, Torii, Mana, Iohara, Keishi, Koizumi, Daisuke, Togawa, Rie, Aizawa, Mamoru, Honda, Michiyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6747779/
https://www.ncbi.nlm.nih.gov/pubmed/31480654
http://dx.doi.org/10.3390/ma12172816
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author Fujiki, Masashi
Abe, Kodai
Hayakawa, Tohru
Yamamoto, Takatsugu
Torii, Mana
Iohara, Keishi
Koizumi, Daisuke
Togawa, Rie
Aizawa, Mamoru
Honda, Michiyo
author_facet Fujiki, Masashi
Abe, Kodai
Hayakawa, Tohru
Yamamoto, Takatsugu
Torii, Mana
Iohara, Keishi
Koizumi, Daisuke
Togawa, Rie
Aizawa, Mamoru
Honda, Michiyo
author_sort Fujiki, Masashi
collection PubMed
description Protamine is an antimicrobial peptide extracted from fish. In this study, we loaded protamine onto dicalcium phosphate anhydride (DCPA), a dental material. Protamine was loaded by stirring DCPA into a protamine solution. To explore the antimicrobial activity of the materials, we cultivated Streptococcus mutans on fabricated discs for 24 h. When S. mutans was cultivated on the discs under no sucrose conditions, the loaded protamine was not released, and the ratio of dead bacteria increased on the surface of P (125) DCPA (half of the saturated level of protamine (125 ppm protamine) was loaded). Aside from P (500) DCPA (saturated level of protamine was loaded), some protamine was released, and the number of planktonic bacteria in the supernatant decreased. Using medium containing 1% sucrose, the release of protamine was promoted from P (125) DCPA due to lowered pH. However, lowering of the pH decreased the antimicrobial activity of protamine. On the other hand, P (500) DCPA released protamine before the pH was lowered, and biofilm formation was inhibited. The loaded protamine expressed antimicrobial activity, both on the surface of the materials and in the surrounding environment. The interaction of loaded protamine with calcium phosphates could promote the application of protamine in the dental field.
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spelling pubmed-67477792019-09-27 Antimicrobial Activity of Protamine-Loaded Calcium Phosphates against Oral Bacteria Fujiki, Masashi Abe, Kodai Hayakawa, Tohru Yamamoto, Takatsugu Torii, Mana Iohara, Keishi Koizumi, Daisuke Togawa, Rie Aizawa, Mamoru Honda, Michiyo Materials (Basel) Article Protamine is an antimicrobial peptide extracted from fish. In this study, we loaded protamine onto dicalcium phosphate anhydride (DCPA), a dental material. Protamine was loaded by stirring DCPA into a protamine solution. To explore the antimicrobial activity of the materials, we cultivated Streptococcus mutans on fabricated discs for 24 h. When S. mutans was cultivated on the discs under no sucrose conditions, the loaded protamine was not released, and the ratio of dead bacteria increased on the surface of P (125) DCPA (half of the saturated level of protamine (125 ppm protamine) was loaded). Aside from P (500) DCPA (saturated level of protamine was loaded), some protamine was released, and the number of planktonic bacteria in the supernatant decreased. Using medium containing 1% sucrose, the release of protamine was promoted from P (125) DCPA due to lowered pH. However, lowering of the pH decreased the antimicrobial activity of protamine. On the other hand, P (500) DCPA released protamine before the pH was lowered, and biofilm formation was inhibited. The loaded protamine expressed antimicrobial activity, both on the surface of the materials and in the surrounding environment. The interaction of loaded protamine with calcium phosphates could promote the application of protamine in the dental field. MDPI 2019-09-02 /pmc/articles/PMC6747779/ /pubmed/31480654 http://dx.doi.org/10.3390/ma12172816 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fujiki, Masashi
Abe, Kodai
Hayakawa, Tohru
Yamamoto, Takatsugu
Torii, Mana
Iohara, Keishi
Koizumi, Daisuke
Togawa, Rie
Aizawa, Mamoru
Honda, Michiyo
Antimicrobial Activity of Protamine-Loaded Calcium Phosphates against Oral Bacteria
title Antimicrobial Activity of Protamine-Loaded Calcium Phosphates against Oral Bacteria
title_full Antimicrobial Activity of Protamine-Loaded Calcium Phosphates against Oral Bacteria
title_fullStr Antimicrobial Activity of Protamine-Loaded Calcium Phosphates against Oral Bacteria
title_full_unstemmed Antimicrobial Activity of Protamine-Loaded Calcium Phosphates against Oral Bacteria
title_short Antimicrobial Activity of Protamine-Loaded Calcium Phosphates against Oral Bacteria
title_sort antimicrobial activity of protamine-loaded calcium phosphates against oral bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6747779/
https://www.ncbi.nlm.nih.gov/pubmed/31480654
http://dx.doi.org/10.3390/ma12172816
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