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Using DNA barcoding to improve invasive pest identification at U.S. ports-of-entry

Interception of potential invasive species at ports-of-entry is essential for effective biosecurity and biosurveillance programs. However, taxonomic assessment of the immature stages of most arthropods is challenging; characters for identification are often dependent on adult morphology and reproduc...

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Autores principales: Madden, Mary J. L., Young, Robert G., Brown, John W., Miller, Scott E., Frewin, Andrew J., Hanner, Robert H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748562/
https://www.ncbi.nlm.nih.gov/pubmed/31527883
http://dx.doi.org/10.1371/journal.pone.0222291
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author Madden, Mary J. L.
Young, Robert G.
Brown, John W.
Miller, Scott E.
Frewin, Andrew J.
Hanner, Robert H.
author_facet Madden, Mary J. L.
Young, Robert G.
Brown, John W.
Miller, Scott E.
Frewin, Andrew J.
Hanner, Robert H.
author_sort Madden, Mary J. L.
collection PubMed
description Interception of potential invasive species at ports-of-entry is essential for effective biosecurity and biosurveillance programs. However, taxonomic assessment of the immature stages of most arthropods is challenging; characters for identification are often dependent on adult morphology and reproductive structures. This study aims to strengthen the identification of such specimens through DNA barcoding, with a focus on microlepidoptera. A sample of 241 primarily immature microlepidoptera specimens intercepted at U.S. ports-of-entry from 2007 to 2011 were selected for analysis. From this sample, 201 COI-5P sequences were generated and analyzed for concordance between morphology-based and DNA-based identifications. The retrospective analysis of the data over 10 years (2009 to 2019) using the Barcode of Life Data (BOLD) system demonstrates the importance of establishing and growing DNA barcode reference libraries for use in specimen identification. Additionally, analysis of specimen identification using public data (43.3% specimens identified) vs. non-public data (78.6% specimens identified) highlights the need to encourage researchers to make data publicly accessible. DNA barcoding surpassed morphological identification with 42.3% (public) and 66.7% (non-public) of the sampled specimens achieving a species-level identification, compared to 38.3% species-level identification by morphology. Whilst DNA barcoding was not able to identify all specimens in our dataset, its incorporation into border security programs as an adjunct to morphological identification can provide secondary lines of evidence and lower taxonomic resolution in many cases. Furthermore, with increased globalization, database records need to be clearly annotated for suspected specimen origin versus interception location.
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spelling pubmed-67485622019-09-27 Using DNA barcoding to improve invasive pest identification at U.S. ports-of-entry Madden, Mary J. L. Young, Robert G. Brown, John W. Miller, Scott E. Frewin, Andrew J. Hanner, Robert H. PLoS One Research Article Interception of potential invasive species at ports-of-entry is essential for effective biosecurity and biosurveillance programs. However, taxonomic assessment of the immature stages of most arthropods is challenging; characters for identification are often dependent on adult morphology and reproductive structures. This study aims to strengthen the identification of such specimens through DNA barcoding, with a focus on microlepidoptera. A sample of 241 primarily immature microlepidoptera specimens intercepted at U.S. ports-of-entry from 2007 to 2011 were selected for analysis. From this sample, 201 COI-5P sequences were generated and analyzed for concordance between morphology-based and DNA-based identifications. The retrospective analysis of the data over 10 years (2009 to 2019) using the Barcode of Life Data (BOLD) system demonstrates the importance of establishing and growing DNA barcode reference libraries for use in specimen identification. Additionally, analysis of specimen identification using public data (43.3% specimens identified) vs. non-public data (78.6% specimens identified) highlights the need to encourage researchers to make data publicly accessible. DNA barcoding surpassed morphological identification with 42.3% (public) and 66.7% (non-public) of the sampled specimens achieving a species-level identification, compared to 38.3% species-level identification by morphology. Whilst DNA barcoding was not able to identify all specimens in our dataset, its incorporation into border security programs as an adjunct to morphological identification can provide secondary lines of evidence and lower taxonomic resolution in many cases. Furthermore, with increased globalization, database records need to be clearly annotated for suspected specimen origin versus interception location. Public Library of Science 2019-09-17 /pmc/articles/PMC6748562/ /pubmed/31527883 http://dx.doi.org/10.1371/journal.pone.0222291 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Madden, Mary J. L.
Young, Robert G.
Brown, John W.
Miller, Scott E.
Frewin, Andrew J.
Hanner, Robert H.
Using DNA barcoding to improve invasive pest identification at U.S. ports-of-entry
title Using DNA barcoding to improve invasive pest identification at U.S. ports-of-entry
title_full Using DNA barcoding to improve invasive pest identification at U.S. ports-of-entry
title_fullStr Using DNA barcoding to improve invasive pest identification at U.S. ports-of-entry
title_full_unstemmed Using DNA barcoding to improve invasive pest identification at U.S. ports-of-entry
title_short Using DNA barcoding to improve invasive pest identification at U.S. ports-of-entry
title_sort using dna barcoding to improve invasive pest identification at u.s. ports-of-entry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748562/
https://www.ncbi.nlm.nih.gov/pubmed/31527883
http://dx.doi.org/10.1371/journal.pone.0222291
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