Reaction of endogenous Coenzyme Q(10) with nitrogen monoxide and its metabolite nitrogen dioxide

Objectives: Coenzyme Q(10), incorporated in DOPC lyposomes or naturally present in liver bovine mitochondria or in human blood plasma, was reacted with nitrogen dioxide (•)NO(2) or with a (•)NO/(•)NO(2) mixture. Methods and Results: The reaction course was monitored by Electron Paramagnetic Resonanc...

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Autores principales: Astolfi, Paola, Clément, Jean-Louis, Gigmes, Didier, Armeni, Tatiana, Carloni, Patricia, Greci, Lucedio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748585/
https://www.ncbi.nlm.nih.gov/pubmed/31348723
http://dx.doi.org/10.1080/13510002.2019.1647005
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author Astolfi, Paola
Clément, Jean-Louis
Gigmes, Didier
Armeni, Tatiana
Carloni, Patricia
Greci, Lucedio
author_facet Astolfi, Paola
Clément, Jean-Louis
Gigmes, Didier
Armeni, Tatiana
Carloni, Patricia
Greci, Lucedio
author_sort Astolfi, Paola
collection PubMed
description Objectives: Coenzyme Q(10), incorporated in DOPC lyposomes or naturally present in liver bovine mitochondria or in human blood plasma, was reacted with nitrogen dioxide (•)NO(2) or with a (•)NO/(•)NO(2) mixture. Methods and Results: The reaction course was monitored by Electron Paramagnetic Resonance (EPR) spectroscopy and in all cases the formation of a di-tert-alkyl nitroxide was observed, deriving from the addition of (•)NO(2) to one of the double bonds, most likely the terminal one, of the isoprenic chain. The rate constant for nitroxide formation was also determined by EPR spectroscopy and an initial rate of ca. 7 × 10(−8 )M s(−1) was obtained.
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spelling pubmed-67485852020-04-15 Reaction of endogenous Coenzyme Q(10) with nitrogen monoxide and its metabolite nitrogen dioxide Astolfi, Paola Clément, Jean-Louis Gigmes, Didier Armeni, Tatiana Carloni, Patricia Greci, Lucedio Redox Rep Research Article Objectives: Coenzyme Q(10), incorporated in DOPC lyposomes or naturally present in liver bovine mitochondria or in human blood plasma, was reacted with nitrogen dioxide (•)NO(2) or with a (•)NO/(•)NO(2) mixture. Methods and Results: The reaction course was monitored by Electron Paramagnetic Resonance (EPR) spectroscopy and in all cases the formation of a di-tert-alkyl nitroxide was observed, deriving from the addition of (•)NO(2) to one of the double bonds, most likely the terminal one, of the isoprenic chain. The rate constant for nitroxide formation was also determined by EPR spectroscopy and an initial rate of ca. 7 × 10(−8 )M s(−1) was obtained. Taylor & Francis 2019-07-26 /pmc/articles/PMC6748585/ /pubmed/31348723 http://dx.doi.org/10.1080/13510002.2019.1647005 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Astolfi, Paola
Clément, Jean-Louis
Gigmes, Didier
Armeni, Tatiana
Carloni, Patricia
Greci, Lucedio
Reaction of endogenous Coenzyme Q(10) with nitrogen monoxide and its metabolite nitrogen dioxide
title Reaction of endogenous Coenzyme Q(10) with nitrogen monoxide and its metabolite nitrogen dioxide
title_full Reaction of endogenous Coenzyme Q(10) with nitrogen monoxide and its metabolite nitrogen dioxide
title_fullStr Reaction of endogenous Coenzyme Q(10) with nitrogen monoxide and its metabolite nitrogen dioxide
title_full_unstemmed Reaction of endogenous Coenzyme Q(10) with nitrogen monoxide and its metabolite nitrogen dioxide
title_short Reaction of endogenous Coenzyme Q(10) with nitrogen monoxide and its metabolite nitrogen dioxide
title_sort reaction of endogenous coenzyme q(10) with nitrogen monoxide and its metabolite nitrogen dioxide
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748585/
https://www.ncbi.nlm.nih.gov/pubmed/31348723
http://dx.doi.org/10.1080/13510002.2019.1647005
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