Cargando…

Antioxidant potential of theaflavin ameliorates the activities of key enzymes of glucose metabolism in high fat diet and streptozotocin – induced diabetic rats

Objectives: The present study was to evaluate the effect of theaflavin on the activities of key enzymes of carbohydrate metabolism in high fat diet and streptozotocin – induced diabetic rats. Methods: Diabetes was induced in male albino Wistar rats by feeding them with high fat diet comprising of st...

Descripción completa

Detalles Bibliográficos
Autores principales: Gothandam, Kirubananthan, Ganesan, Vijayan Siva, Ayyasamy, Thangaraj, Ramalingam, Sundaram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748596/
https://www.ncbi.nlm.nih.gov/pubmed/31142215
http://dx.doi.org/10.1080/13510002.2019.1624085
_version_ 1783452122772144128
author Gothandam, Kirubananthan
Ganesan, Vijayan Siva
Ayyasamy, Thangaraj
Ramalingam, Sundaram
author_facet Gothandam, Kirubananthan
Ganesan, Vijayan Siva
Ayyasamy, Thangaraj
Ramalingam, Sundaram
author_sort Gothandam, Kirubananthan
collection PubMed
description Objectives: The present study was to evaluate the effect of theaflavin on the activities of key enzymes of carbohydrate metabolism in high fat diet and streptozotocin – induced diabetic rats. Methods: Diabetes was induced in male albino Wistar rats by feeding them with high fat diet comprising of standard laboratory rat chow 84.3%, lard 5%, egg yolk powder 10%, cholesterol 0.2% and bile salt 0.5% for 2 weeks. After 2 weeks, the animals were kept in an overnight fast and injected with low dose of streptozotocin (40 mg/kg b.w). Results: Theaflavin (100 mg/kg b.w /day) was administered orally to diabetic rats for 30 days. At the end of the experimental period, diabetic control rats showed significant increase in plasma glucose, homeostatic model assessment of insulin resistance (HOMA-IR), glycosylated hemoglobin (HbA1c) with concomitant decrease in plasma insulin, total hemoglobin and body weight. The activities of key enzymes of carbohydrate metabolism, lipid peroxidation markers, antioxidant enzymes, glycogen content and glycogen synthase and glycogen phosphorylase were also altered in diabetic rats. Discussion: Oral administration of theaflavin to diabetic rats significantly ameliorated all the biochemical alterations to near normal levels. The results of the present study suggest that theaflavin exhibits antidiabetic effect through its antioxidant activity.
format Online
Article
Text
id pubmed-6748596
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-67485962020-04-15 Antioxidant potential of theaflavin ameliorates the activities of key enzymes of glucose metabolism in high fat diet and streptozotocin – induced diabetic rats Gothandam, Kirubananthan Ganesan, Vijayan Siva Ayyasamy, Thangaraj Ramalingam, Sundaram Redox Rep Research Article Objectives: The present study was to evaluate the effect of theaflavin on the activities of key enzymes of carbohydrate metabolism in high fat diet and streptozotocin – induced diabetic rats. Methods: Diabetes was induced in male albino Wistar rats by feeding them with high fat diet comprising of standard laboratory rat chow 84.3%, lard 5%, egg yolk powder 10%, cholesterol 0.2% and bile salt 0.5% for 2 weeks. After 2 weeks, the animals were kept in an overnight fast and injected with low dose of streptozotocin (40 mg/kg b.w). Results: Theaflavin (100 mg/kg b.w /day) was administered orally to diabetic rats for 30 days. At the end of the experimental period, diabetic control rats showed significant increase in plasma glucose, homeostatic model assessment of insulin resistance (HOMA-IR), glycosylated hemoglobin (HbA1c) with concomitant decrease in plasma insulin, total hemoglobin and body weight. The activities of key enzymes of carbohydrate metabolism, lipid peroxidation markers, antioxidant enzymes, glycogen content and glycogen synthase and glycogen phosphorylase were also altered in diabetic rats. Discussion: Oral administration of theaflavin to diabetic rats significantly ameliorated all the biochemical alterations to near normal levels. The results of the present study suggest that theaflavin exhibits antidiabetic effect through its antioxidant activity. Taylor & Francis 2019-05-29 /pmc/articles/PMC6748596/ /pubmed/31142215 http://dx.doi.org/10.1080/13510002.2019.1624085 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gothandam, Kirubananthan
Ganesan, Vijayan Siva
Ayyasamy, Thangaraj
Ramalingam, Sundaram
Antioxidant potential of theaflavin ameliorates the activities of key enzymes of glucose metabolism in high fat diet and streptozotocin – induced diabetic rats
title Antioxidant potential of theaflavin ameliorates the activities of key enzymes of glucose metabolism in high fat diet and streptozotocin – induced diabetic rats
title_full Antioxidant potential of theaflavin ameliorates the activities of key enzymes of glucose metabolism in high fat diet and streptozotocin – induced diabetic rats
title_fullStr Antioxidant potential of theaflavin ameliorates the activities of key enzymes of glucose metabolism in high fat diet and streptozotocin – induced diabetic rats
title_full_unstemmed Antioxidant potential of theaflavin ameliorates the activities of key enzymes of glucose metabolism in high fat diet and streptozotocin – induced diabetic rats
title_short Antioxidant potential of theaflavin ameliorates the activities of key enzymes of glucose metabolism in high fat diet and streptozotocin – induced diabetic rats
title_sort antioxidant potential of theaflavin ameliorates the activities of key enzymes of glucose metabolism in high fat diet and streptozotocin – induced diabetic rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748596/
https://www.ncbi.nlm.nih.gov/pubmed/31142215
http://dx.doi.org/10.1080/13510002.2019.1624085
work_keys_str_mv AT gothandamkirubananthan antioxidantpotentialoftheaflavinamelioratestheactivitiesofkeyenzymesofglucosemetabolisminhighfatdietandstreptozotocininduceddiabeticrats
AT ganesanvijayansiva antioxidantpotentialoftheaflavinamelioratestheactivitiesofkeyenzymesofglucosemetabolisminhighfatdietandstreptozotocininduceddiabeticrats
AT ayyasamythangaraj antioxidantpotentialoftheaflavinamelioratestheactivitiesofkeyenzymesofglucosemetabolisminhighfatdietandstreptozotocininduceddiabeticrats
AT ramalingamsundaram antioxidantpotentialoftheaflavinamelioratestheactivitiesofkeyenzymesofglucosemetabolisminhighfatdietandstreptozotocininduceddiabeticrats