Multispectral tracing in densely labeled mouse brain with nTracer

SUMMARY: This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of multispectral fluorescent tissue samples. This approach allows for rapid and accurate reconstruction of whole cell morphology of large neuronal populations in densely labeled brains. AVAILABILITY AND I...

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Detalles Bibliográficos
Autores principales: Roossien, Douglas H, Sadis, Benjamin V, Yan, Yan, Webb, John M, Min, Lia Y, Dizaji, Aslan S, Bogart, Luke J, Mazuski, Cristina, Huth, Robert S, Stecher, Johanna S, Akula, Sriakhila, Shen, Fred, Li, Ye, Xiao, Tingxin, Vandenbrink, Madeleine, Lichtman, Jeff W, Hensch, Takao K, Herzog, Erik D, Cai, Dawen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Applications Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748755/
https://www.ncbi.nlm.nih.gov/pubmed/30715234
http://dx.doi.org/10.1093/bioinformatics/btz084
Descripción
Sumario:SUMMARY: This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of multispectral fluorescent tissue samples. This approach allows for rapid and accurate reconstruction of whole cell morphology of large neuronal populations in densely labeled brains. AVAILABILITY AND IMPLEMENTATION: nTracer was written as a plug-in for the open source image processing software ImageJ. The software, instructional documentation, tutorial videos, sample image and sample tracing results are available at https://www.cai-lab.org/ntracer-tutorial. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

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