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A contractile injection system stimulates tubeworm metamorphosis by translocating a proteinaceous effector

The swimming larvae of many marine animals identify a location on the sea floor to undergo metamorphosis based on the presence of specific bacteria. Although this microbe–animal interaction is critical for the life cycles of diverse marine animals, what types of biochemical cues from bacteria that i...

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Autores principales: Ericson, Charles F, Eisenstein, Fabian, Medeiros, João M, Malter, Kyle E, Cavalcanti, Giselle S, Zeller, Robert W, Newman, Dianne K, Pilhofer, Martin, Shikuma, Nicholas J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748791/
https://www.ncbi.nlm.nih.gov/pubmed/31526475
http://dx.doi.org/10.7554/eLife.46845
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author Ericson, Charles F
Eisenstein, Fabian
Medeiros, João M
Malter, Kyle E
Cavalcanti, Giselle S
Zeller, Robert W
Newman, Dianne K
Pilhofer, Martin
Shikuma, Nicholas J
author_facet Ericson, Charles F
Eisenstein, Fabian
Medeiros, João M
Malter, Kyle E
Cavalcanti, Giselle S
Zeller, Robert W
Newman, Dianne K
Pilhofer, Martin
Shikuma, Nicholas J
author_sort Ericson, Charles F
collection PubMed
description The swimming larvae of many marine animals identify a location on the sea floor to undergo metamorphosis based on the presence of specific bacteria. Although this microbe–animal interaction is critical for the life cycles of diverse marine animals, what types of biochemical cues from bacteria that induce metamorphosis has been a mystery. Metamorphosis of larvae of the tubeworm Hydroides elegans is induced by arrays of phage tail-like contractile injection systems, which are released by the bacterium Pseudoalteromonas luteoviolacea. Here we identify the novel effector protein Mif1. By cryo-electron tomography imaging and functional assays, we observe Mif1 as cargo inside the tube lumen of the contractile injection system and show that the mif1 gene is required for inducing metamorphosis. Purified Mif1 is sufficient for triggering metamorphosis when electroporated into tubeworm larvae. Our results indicate that the delivery of protein effectors by contractile injection systems may orchestrate microbe–animal interactions in diverse contexts.
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spelling pubmed-67487912019-09-20 A contractile injection system stimulates tubeworm metamorphosis by translocating a proteinaceous effector Ericson, Charles F Eisenstein, Fabian Medeiros, João M Malter, Kyle E Cavalcanti, Giselle S Zeller, Robert W Newman, Dianne K Pilhofer, Martin Shikuma, Nicholas J eLife Microbiology and Infectious Disease The swimming larvae of many marine animals identify a location on the sea floor to undergo metamorphosis based on the presence of specific bacteria. Although this microbe–animal interaction is critical for the life cycles of diverse marine animals, what types of biochemical cues from bacteria that induce metamorphosis has been a mystery. Metamorphosis of larvae of the tubeworm Hydroides elegans is induced by arrays of phage tail-like contractile injection systems, which are released by the bacterium Pseudoalteromonas luteoviolacea. Here we identify the novel effector protein Mif1. By cryo-electron tomography imaging and functional assays, we observe Mif1 as cargo inside the tube lumen of the contractile injection system and show that the mif1 gene is required for inducing metamorphosis. Purified Mif1 is sufficient for triggering metamorphosis when electroporated into tubeworm larvae. Our results indicate that the delivery of protein effectors by contractile injection systems may orchestrate microbe–animal interactions in diverse contexts. eLife Sciences Publications, Ltd 2019-09-17 /pmc/articles/PMC6748791/ /pubmed/31526475 http://dx.doi.org/10.7554/eLife.46845 Text en © 2019, Ericson et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Microbiology and Infectious Disease
Ericson, Charles F
Eisenstein, Fabian
Medeiros, João M
Malter, Kyle E
Cavalcanti, Giselle S
Zeller, Robert W
Newman, Dianne K
Pilhofer, Martin
Shikuma, Nicholas J
A contractile injection system stimulates tubeworm metamorphosis by translocating a proteinaceous effector
title A contractile injection system stimulates tubeworm metamorphosis by translocating a proteinaceous effector
title_full A contractile injection system stimulates tubeworm metamorphosis by translocating a proteinaceous effector
title_fullStr A contractile injection system stimulates tubeworm metamorphosis by translocating a proteinaceous effector
title_full_unstemmed A contractile injection system stimulates tubeworm metamorphosis by translocating a proteinaceous effector
title_short A contractile injection system stimulates tubeworm metamorphosis by translocating a proteinaceous effector
title_sort contractile injection system stimulates tubeworm metamorphosis by translocating a proteinaceous effector
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748791/
https://www.ncbi.nlm.nih.gov/pubmed/31526475
http://dx.doi.org/10.7554/eLife.46845
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