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Increased Granulopoiesis in the Bone Marrow following Epstein-Barr Virus Infection

Epstein-Barr virus (EBV) is associated with several disorders. EBV is known to modulate the proliferation and survival of hematopoietic cells such as B cells and T cells in human. However, the effects of EBV on hematopoiesis itself have not been investigated. To study EBV infection in murine models,...

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Autores principales: Katahira, Yasuhiro, Higuchi, Hiroshi, Matsushita, Hiromichi, Yahata, Takashi, Yamamoto, Yuichiro, Koike, Ryo, Ando, Kiyoshi, Sato, Katsuaki, Imadome, Ken-Ichi, Kotani, Ai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748920/
https://www.ncbi.nlm.nih.gov/pubmed/31530932
http://dx.doi.org/10.1038/s41598-019-49937-w
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author Katahira, Yasuhiro
Higuchi, Hiroshi
Matsushita, Hiromichi
Yahata, Takashi
Yamamoto, Yuichiro
Koike, Ryo
Ando, Kiyoshi
Sato, Katsuaki
Imadome, Ken-Ichi
Kotani, Ai
author_facet Katahira, Yasuhiro
Higuchi, Hiroshi
Matsushita, Hiromichi
Yahata, Takashi
Yamamoto, Yuichiro
Koike, Ryo
Ando, Kiyoshi
Sato, Katsuaki
Imadome, Ken-Ichi
Kotani, Ai
author_sort Katahira, Yasuhiro
collection PubMed
description Epstein-Barr virus (EBV) is associated with several disorders. EBV is known to modulate the proliferation and survival of hematopoietic cells such as B cells and T cells in human. However, the effects of EBV on hematopoiesis itself have not been investigated. To study EBV infection in murine models, their hematopoiesis must be humanized, since EBV infection is limited only in primates. To engraft the human hematopoiesis, NOD/Shi-scid-IL2rγ(null) (NOG) mice were used. Usually, the hematopoiesis humanized mice reconstitute only lymphoid cells, but myeloid cells are not. However, we revealed human macrophages (hMφ) and their precursor monocytes were increased in peripheral tissues of EBV-infected mice. Furthermore, our previous report indicated Mφ accumulation in spleen was essential for development of EBV-positive tumors, suggesting that EBV modulates human hematopoiesis in order to thrive. Interestingly, we revealed a dramatic increase of immature granulocytes only in bone marrow of EBV-infected mice. In addition, GM-CSF, a cytokine that is essential for differentiation of the myeloid lineage, was significantly increased in EBV-infected mice. These results were also reproduced in patients with EBV-related disorders. We suggest that the hematopoietic alterations during EBV-infection might contribute immune suppression to the development and exacerbation of EBV-related disorders.
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spelling pubmed-67489202019-09-27 Increased Granulopoiesis in the Bone Marrow following Epstein-Barr Virus Infection Katahira, Yasuhiro Higuchi, Hiroshi Matsushita, Hiromichi Yahata, Takashi Yamamoto, Yuichiro Koike, Ryo Ando, Kiyoshi Sato, Katsuaki Imadome, Ken-Ichi Kotani, Ai Sci Rep Article Epstein-Barr virus (EBV) is associated with several disorders. EBV is known to modulate the proliferation and survival of hematopoietic cells such as B cells and T cells in human. However, the effects of EBV on hematopoiesis itself have not been investigated. To study EBV infection in murine models, their hematopoiesis must be humanized, since EBV infection is limited only in primates. To engraft the human hematopoiesis, NOD/Shi-scid-IL2rγ(null) (NOG) mice were used. Usually, the hematopoiesis humanized mice reconstitute only lymphoid cells, but myeloid cells are not. However, we revealed human macrophages (hMφ) and their precursor monocytes were increased in peripheral tissues of EBV-infected mice. Furthermore, our previous report indicated Mφ accumulation in spleen was essential for development of EBV-positive tumors, suggesting that EBV modulates human hematopoiesis in order to thrive. Interestingly, we revealed a dramatic increase of immature granulocytes only in bone marrow of EBV-infected mice. In addition, GM-CSF, a cytokine that is essential for differentiation of the myeloid lineage, was significantly increased in EBV-infected mice. These results were also reproduced in patients with EBV-related disorders. We suggest that the hematopoietic alterations during EBV-infection might contribute immune suppression to the development and exacerbation of EBV-related disorders. Nature Publishing Group UK 2019-09-17 /pmc/articles/PMC6748920/ /pubmed/31530932 http://dx.doi.org/10.1038/s41598-019-49937-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Katahira, Yasuhiro
Higuchi, Hiroshi
Matsushita, Hiromichi
Yahata, Takashi
Yamamoto, Yuichiro
Koike, Ryo
Ando, Kiyoshi
Sato, Katsuaki
Imadome, Ken-Ichi
Kotani, Ai
Increased Granulopoiesis in the Bone Marrow following Epstein-Barr Virus Infection
title Increased Granulopoiesis in the Bone Marrow following Epstein-Barr Virus Infection
title_full Increased Granulopoiesis in the Bone Marrow following Epstein-Barr Virus Infection
title_fullStr Increased Granulopoiesis in the Bone Marrow following Epstein-Barr Virus Infection
title_full_unstemmed Increased Granulopoiesis in the Bone Marrow following Epstein-Barr Virus Infection
title_short Increased Granulopoiesis in the Bone Marrow following Epstein-Barr Virus Infection
title_sort increased granulopoiesis in the bone marrow following epstein-barr virus infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748920/
https://www.ncbi.nlm.nih.gov/pubmed/31530932
http://dx.doi.org/10.1038/s41598-019-49937-w
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