Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor

The exponential escalation of dengue cases has indeed become a global health crisis. This work elaborates on the development of a biofunctionalized tapered optical fiber (TOF) based sensor with the integration of polyamidoamine (PAMAM) dendrimer for the detection of dengue E protein. The dimension o...

Descripción completa

Detalles Bibliográficos
Autores principales: Mustapha Kamil, Yasmin, Al-Rekabi, Sura H., Yaacob, Mohd Hanif, Syahir, Amir, Chee, Hui Yee, Mahdi, Mohd Adzir, Abu Bakar, Muhammad Hafiz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748962/
https://www.ncbi.nlm.nih.gov/pubmed/31530893
http://dx.doi.org/10.1038/s41598-019-49891-7
_version_ 1783452175528099840
author Mustapha Kamil, Yasmin
Al-Rekabi, Sura H.
Yaacob, Mohd Hanif
Syahir, Amir
Chee, Hui Yee
Mahdi, Mohd Adzir
Abu Bakar, Muhammad Hafiz
author_facet Mustapha Kamil, Yasmin
Al-Rekabi, Sura H.
Yaacob, Mohd Hanif
Syahir, Amir
Chee, Hui Yee
Mahdi, Mohd Adzir
Abu Bakar, Muhammad Hafiz
author_sort Mustapha Kamil, Yasmin
collection PubMed
description The exponential escalation of dengue cases has indeed become a global health crisis. This work elaborates on the development of a biofunctionalized tapered optical fiber (TOF) based sensor with the integration of polyamidoamine (PAMAM) dendrimer for the detection of dengue E protein. The dimension of the TOF generated an evanescent field that was sensitive to any changes in the external medium while the integration of PAMAM promoted more adhesion of bio-recognition molecules; anti-DENV II E protein antibodies; that were complementary to the targeted protein. This in return created more active sites for the absorption of DENV II E proteins onto the tapered region. The resolution and detection limit of the sensor are 19.53 nm/nM and 1 pM, respectively with K(d) = 1.02 × 10(−10) M.
format Online
Article
Text
id pubmed-6748962
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-67489622019-09-27 Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor Mustapha Kamil, Yasmin Al-Rekabi, Sura H. Yaacob, Mohd Hanif Syahir, Amir Chee, Hui Yee Mahdi, Mohd Adzir Abu Bakar, Muhammad Hafiz Sci Rep Article The exponential escalation of dengue cases has indeed become a global health crisis. This work elaborates on the development of a biofunctionalized tapered optical fiber (TOF) based sensor with the integration of polyamidoamine (PAMAM) dendrimer for the detection of dengue E protein. The dimension of the TOF generated an evanescent field that was sensitive to any changes in the external medium while the integration of PAMAM promoted more adhesion of bio-recognition molecules; anti-DENV II E protein antibodies; that were complementary to the targeted protein. This in return created more active sites for the absorption of DENV II E proteins onto the tapered region. The resolution and detection limit of the sensor are 19.53 nm/nM and 1 pM, respectively with K(d) = 1.02 × 10(−10) M. Nature Publishing Group UK 2019-09-17 /pmc/articles/PMC6748962/ /pubmed/31530893 http://dx.doi.org/10.1038/s41598-019-49891-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mustapha Kamil, Yasmin
Al-Rekabi, Sura H.
Yaacob, Mohd Hanif
Syahir, Amir
Chee, Hui Yee
Mahdi, Mohd Adzir
Abu Bakar, Muhammad Hafiz
Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor
title Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor
title_full Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor
title_fullStr Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor
title_full_unstemmed Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor
title_short Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor
title_sort detection of dengue using pamam dendrimer integrated tapered optical fiber sensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748962/
https://www.ncbi.nlm.nih.gov/pubmed/31530893
http://dx.doi.org/10.1038/s41598-019-49891-7
work_keys_str_mv AT mustaphakamilyasmin detectionofdengueusingpamamdendrimerintegratedtaperedopticalfibersensor
AT alrekabisurah detectionofdengueusingpamamdendrimerintegratedtaperedopticalfibersensor
AT yaacobmohdhanif detectionofdengueusingpamamdendrimerintegratedtaperedopticalfibersensor
AT syahiramir detectionofdengueusingpamamdendrimerintegratedtaperedopticalfibersensor
AT cheehuiyee detectionofdengueusingpamamdendrimerintegratedtaperedopticalfibersensor
AT mahdimohdadzir detectionofdengueusingpamamdendrimerintegratedtaperedopticalfibersensor
AT abubakarmuhammadhafiz detectionofdengueusingpamamdendrimerintegratedtaperedopticalfibersensor

Ejemplares similares