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Quantitative expression of MMPs 2, 9, 14, and collagen IV in LCIS and paired normal breast tissue
Certain matrix metalloproteinases (MMPs) have the ability to degrade collagen IV, a main component of the breast lobular basement membrane. In this cross-sectional study, we evaluated expression of MMPs 2, 9, and 14 and collagen IV in LCIS and adjacent normal breast tissue among LCIS patients withou...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748975/ https://www.ncbi.nlm.nih.gov/pubmed/31530842 http://dx.doi.org/10.1038/s41598-019-48602-6 |
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author | Nyante, Sarah J. Wang, Tengteng Tan, Xianming Ozdowski, Emily F. Lawton, Thomas J. |
author_facet | Nyante, Sarah J. Wang, Tengteng Tan, Xianming Ozdowski, Emily F. Lawton, Thomas J. |
author_sort | Nyante, Sarah J. |
collection | PubMed |
description | Certain matrix metalloproteinases (MMPs) have the ability to degrade collagen IV, a main component of the breast lobular basement membrane. In this cross-sectional study, we evaluated expression of MMPs 2, 9, and 14 and collagen IV in LCIS and adjacent normal breast tissue among LCIS patients without invasive breast cancer to determine whether expression differed between benign and preinvasive breast epithelial tissue. A total of 64 LCIS patients, diagnosed 2004–2014, were included; 44 had sufficient paired normal tissue for analysis. Marker epithelial expression was measured using immunofluorescence and quantified using the H score (MMPs) or pixel intensity (collagen IV). Associations were evaluated using the Spearman correlation or the Wilcoxon signed-rank test. In LCIS and normal tissue, there was a strong correlation between MMP2 and MMP14 expression (LCIS r = 0.69, normal r = 0.81, both P < 0.01). Other pairwise correlations were moderate to weak (range: LCIS r = 0.32–0.47, normal r = 0.19–0.32). For all markers, expression was lower in LCIS vs. normal tissue (all P ≤ 0.05). In sum, collagenase MMPs were expressed in normal breast and LCIS lesions of LCIS patients. However, expression was not higher in LCIS compared with normal tissue, suggesting collagenase MMP expression does not increase as breast tissue gains a more proliferative phenotype. |
format | Online Article Text |
id | pubmed-6748975 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67489752019-09-27 Quantitative expression of MMPs 2, 9, 14, and collagen IV in LCIS and paired normal breast tissue Nyante, Sarah J. Wang, Tengteng Tan, Xianming Ozdowski, Emily F. Lawton, Thomas J. Sci Rep Article Certain matrix metalloproteinases (MMPs) have the ability to degrade collagen IV, a main component of the breast lobular basement membrane. In this cross-sectional study, we evaluated expression of MMPs 2, 9, and 14 and collagen IV in LCIS and adjacent normal breast tissue among LCIS patients without invasive breast cancer to determine whether expression differed between benign and preinvasive breast epithelial tissue. A total of 64 LCIS patients, diagnosed 2004–2014, were included; 44 had sufficient paired normal tissue for analysis. Marker epithelial expression was measured using immunofluorescence and quantified using the H score (MMPs) or pixel intensity (collagen IV). Associations were evaluated using the Spearman correlation or the Wilcoxon signed-rank test. In LCIS and normal tissue, there was a strong correlation between MMP2 and MMP14 expression (LCIS r = 0.69, normal r = 0.81, both P < 0.01). Other pairwise correlations were moderate to weak (range: LCIS r = 0.32–0.47, normal r = 0.19–0.32). For all markers, expression was lower in LCIS vs. normal tissue (all P ≤ 0.05). In sum, collagenase MMPs were expressed in normal breast and LCIS lesions of LCIS patients. However, expression was not higher in LCIS compared with normal tissue, suggesting collagenase MMP expression does not increase as breast tissue gains a more proliferative phenotype. Nature Publishing Group UK 2019-09-17 /pmc/articles/PMC6748975/ /pubmed/31530842 http://dx.doi.org/10.1038/s41598-019-48602-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Nyante, Sarah J. Wang, Tengteng Tan, Xianming Ozdowski, Emily F. Lawton, Thomas J. Quantitative expression of MMPs 2, 9, 14, and collagen IV in LCIS and paired normal breast tissue |
title | Quantitative expression of MMPs 2, 9, 14, and collagen IV in LCIS and paired normal breast tissue |
title_full | Quantitative expression of MMPs 2, 9, 14, and collagen IV in LCIS and paired normal breast tissue |
title_fullStr | Quantitative expression of MMPs 2, 9, 14, and collagen IV in LCIS and paired normal breast tissue |
title_full_unstemmed | Quantitative expression of MMPs 2, 9, 14, and collagen IV in LCIS and paired normal breast tissue |
title_short | Quantitative expression of MMPs 2, 9, 14, and collagen IV in LCIS and paired normal breast tissue |
title_sort | quantitative expression of mmps 2, 9, 14, and collagen iv in lcis and paired normal breast tissue |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748975/ https://www.ncbi.nlm.nih.gov/pubmed/31530842 http://dx.doi.org/10.1038/s41598-019-48602-6 |
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