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Testing the Clinical Applicability of Resin Infiltration of Developmental Enamel Hypomineralization Lesions Using an In Vitro Model

AIM: The aim of this study is to investigate the penetration abilities of a commercially available low-viscosity resin infiltrant into developmentally hypomineralized teeth in vitro. MATERIALS AND METHODS: Four extracted third molars of a 17-year-old patient with signs of developmental enamel hypomi...

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Autores principales: Schnabl, Dagmar, Dudasne-Orosz, Viktoria, Glueckert, Rudolf, Handschuh, Stephan, Kapferer-Seebacher, Ines, Dumfahrt, Herbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Jaypee Brothers Medical Publishers 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6749872/
https://www.ncbi.nlm.nih.gov/pubmed/31571785
http://dx.doi.org/10.5005/jp-journals-10005-1609
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author Schnabl, Dagmar
Dudasne-Orosz, Viktoria
Glueckert, Rudolf
Handschuh, Stephan
Kapferer-Seebacher, Ines
Dumfahrt, Herbert
author_facet Schnabl, Dagmar
Dudasne-Orosz, Viktoria
Glueckert, Rudolf
Handschuh, Stephan
Kapferer-Seebacher, Ines
Dumfahrt, Herbert
author_sort Schnabl, Dagmar
collection PubMed
description AIM: The aim of this study is to investigate the penetration abilities of a commercially available low-viscosity resin infiltrant into developmentally hypomineralized teeth in vitro. MATERIALS AND METHODS: Four extracted third molars of a 17-year-old patient with signs of developmental enamel hypomineralization (discoloration, increased opacity, and surface roughness) were infiltrated with a low-viscosity resin mixed with a fluorescent dye, according to the manufacturer's standard protocol. Four extracted molars with sound enamel or showing only initial fissure caries were used as a control group. Specimens were embedded in polymethylmethacrylate, and grindings were prepared. High-resolution projectional radiography of the grindings was performed, and, for one specimen, quantitative micro-computed tomography was used to measure hydroxyapatite density in enamel and dentin lesions. After decalcification, the grindings were examined by reflected bright-field microscopy, wide-field fluorescence microscopy, and confocal laser scanning microscopy. Fluorescence micrographs were superimposed on the radiographs and analyzed correlatively. RESULTS: The pattern of hypo-/demineralization in enamel and dentin in developmentally hypomineralized teeth showed a good congruence with the pattern of resin infiltration. Cavitations and dentin tubules up to a depth of 2 mm beyond cavitations were filled by the infiltrant. In control teeth, the penetration of the infiltrant was limited to decalcified enamel areas (initial fissure caries). CONCLUSIONS: In vitro infiltration of developmentally hypomineralized enamel was successful. CLINICAL SIGNIFICANCE: Resin infiltration might be considered as a routine procedure in the treatment of developmentally hypomineralized teeth. Further investigations with higher sample sizes, different degrees of severity, different stages of lesion extension, and modified treatment protocols are necessary. HOW TO CITE THIS ARTICLE: Schnabl D, Dudasne-Orosz V, et al. Testing the Clinical Applicability of Resin Infiltration of Developmental Enamel Hypomineralization Lesions Using an In Vitro Model. Int J Clin Pediatr Dent 2019;12(2):126–132.
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spelling pubmed-67498722019-09-30 Testing the Clinical Applicability of Resin Infiltration of Developmental Enamel Hypomineralization Lesions Using an In Vitro Model Schnabl, Dagmar Dudasne-Orosz, Viktoria Glueckert, Rudolf Handschuh, Stephan Kapferer-Seebacher, Ines Dumfahrt, Herbert Int J Clin Pediatr Dent Original Article AIM: The aim of this study is to investigate the penetration abilities of a commercially available low-viscosity resin infiltrant into developmentally hypomineralized teeth in vitro. MATERIALS AND METHODS: Four extracted third molars of a 17-year-old patient with signs of developmental enamel hypomineralization (discoloration, increased opacity, and surface roughness) were infiltrated with a low-viscosity resin mixed with a fluorescent dye, according to the manufacturer's standard protocol. Four extracted molars with sound enamel or showing only initial fissure caries were used as a control group. Specimens were embedded in polymethylmethacrylate, and grindings were prepared. High-resolution projectional radiography of the grindings was performed, and, for one specimen, quantitative micro-computed tomography was used to measure hydroxyapatite density in enamel and dentin lesions. After decalcification, the grindings were examined by reflected bright-field microscopy, wide-field fluorescence microscopy, and confocal laser scanning microscopy. Fluorescence micrographs were superimposed on the radiographs and analyzed correlatively. RESULTS: The pattern of hypo-/demineralization in enamel and dentin in developmentally hypomineralized teeth showed a good congruence with the pattern of resin infiltration. Cavitations and dentin tubules up to a depth of 2 mm beyond cavitations were filled by the infiltrant. In control teeth, the penetration of the infiltrant was limited to decalcified enamel areas (initial fissure caries). CONCLUSIONS: In vitro infiltration of developmentally hypomineralized enamel was successful. CLINICAL SIGNIFICANCE: Resin infiltration might be considered as a routine procedure in the treatment of developmentally hypomineralized teeth. Further investigations with higher sample sizes, different degrees of severity, different stages of lesion extension, and modified treatment protocols are necessary. HOW TO CITE THIS ARTICLE: Schnabl D, Dudasne-Orosz V, et al. Testing the Clinical Applicability of Resin Infiltration of Developmental Enamel Hypomineralization Lesions Using an In Vitro Model. Int J Clin Pediatr Dent 2019;12(2):126–132. Jaypee Brothers Medical Publishers 2019 /pmc/articles/PMC6749872/ /pubmed/31571785 http://dx.doi.org/10.5005/jp-journals-10005-1609 Text en Copyright © 2019; Jaypee Brothers Medical Publishers (P) Ltd. © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and non-commercial reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Original Article
Schnabl, Dagmar
Dudasne-Orosz, Viktoria
Glueckert, Rudolf
Handschuh, Stephan
Kapferer-Seebacher, Ines
Dumfahrt, Herbert
Testing the Clinical Applicability of Resin Infiltration of Developmental Enamel Hypomineralization Lesions Using an In Vitro Model
title Testing the Clinical Applicability of Resin Infiltration of Developmental Enamel Hypomineralization Lesions Using an In Vitro Model
title_full Testing the Clinical Applicability of Resin Infiltration of Developmental Enamel Hypomineralization Lesions Using an In Vitro Model
title_fullStr Testing the Clinical Applicability of Resin Infiltration of Developmental Enamel Hypomineralization Lesions Using an In Vitro Model
title_full_unstemmed Testing the Clinical Applicability of Resin Infiltration of Developmental Enamel Hypomineralization Lesions Using an In Vitro Model
title_short Testing the Clinical Applicability of Resin Infiltration of Developmental Enamel Hypomineralization Lesions Using an In Vitro Model
title_sort testing the clinical applicability of resin infiltration of developmental enamel hypomineralization lesions using an in vitro model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6749872/
https://www.ncbi.nlm.nih.gov/pubmed/31571785
http://dx.doi.org/10.5005/jp-journals-10005-1609
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