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A Simple Approach to Bioconjugation at Diverse Levels: Metal-Free Click Reactions of Activated Alkynes with Native Groups of Biotargets without Prefunctionalization
The efficient bioconjugation of functional groups/molecules to targeted matrix and bio-related species drives the great development of material science and biomedicine, while the dilemma of metal catalysis, uneasy premodification, and limited reaction efficiency in traditional bioconjugation has res...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AAAS
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6750040/ https://www.ncbi.nlm.nih.gov/pubmed/31549027 http://dx.doi.org/10.1155/2018/3152870 |
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author | Hu, Xianglong Zhao, Xueqian He, Benzhao Zhao, Zheng Zheng, Zheng Zhang, Pengfei Shi, Xiujuan Kwok, Ryan T. K. Lam, Jacky W. Y. Qin, Anjun Tang, Ben Zhong |
author_facet | Hu, Xianglong Zhao, Xueqian He, Benzhao Zhao, Zheng Zheng, Zheng Zhang, Pengfei Shi, Xiujuan Kwok, Ryan T. K. Lam, Jacky W. Y. Qin, Anjun Tang, Ben Zhong |
author_sort | Hu, Xianglong |
collection | PubMed |
description | The efficient bioconjugation of functional groups/molecules to targeted matrix and bio-related species drives the great development of material science and biomedicine, while the dilemma of metal catalysis, uneasy premodification, and limited reaction efficiency in traditional bioconjugation has restricted the booming development to some extent. Here, we provide a strategy for metal-free click bioconjugation at diverse levels based on activated alkynes. As a proof-of-concept, the abundant native groups including amine, thiol, and hydroxyl groups can directly react with activated alkynes without any modification in the absence of metal catalysis. Through this strategy, high-efficient modification and potential functionalization can be achieved for natural polysaccharide, biocompatible polyethylene glycol (PEG), synthetic polymers, cell penetrating peptide, protein, fast whole-cell mapping, and even quick differentiation and staining of Gram-positive bacteria, etc. Therefore, current metal-free click bioconjugation strategy based on activated alkynes is promising for the development of quick fluorescence labeling and functional modification of many targets and can be widely applied towards the fabrication of complex biomaterials and future in vivo labeling and detection. |
format | Online Article Text |
id | pubmed-6750040 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | AAAS |
record_format | MEDLINE/PubMed |
spelling | pubmed-67500402019-09-23 A Simple Approach to Bioconjugation at Diverse Levels: Metal-Free Click Reactions of Activated Alkynes with Native Groups of Biotargets without Prefunctionalization Hu, Xianglong Zhao, Xueqian He, Benzhao Zhao, Zheng Zheng, Zheng Zhang, Pengfei Shi, Xiujuan Kwok, Ryan T. K. Lam, Jacky W. Y. Qin, Anjun Tang, Ben Zhong Research (Wash D C) Research Article The efficient bioconjugation of functional groups/molecules to targeted matrix and bio-related species drives the great development of material science and biomedicine, while the dilemma of metal catalysis, uneasy premodification, and limited reaction efficiency in traditional bioconjugation has restricted the booming development to some extent. Here, we provide a strategy for metal-free click bioconjugation at diverse levels based on activated alkynes. As a proof-of-concept, the abundant native groups including amine, thiol, and hydroxyl groups can directly react with activated alkynes without any modification in the absence of metal catalysis. Through this strategy, high-efficient modification and potential functionalization can be achieved for natural polysaccharide, biocompatible polyethylene glycol (PEG), synthetic polymers, cell penetrating peptide, protein, fast whole-cell mapping, and even quick differentiation and staining of Gram-positive bacteria, etc. Therefore, current metal-free click bioconjugation strategy based on activated alkynes is promising for the development of quick fluorescence labeling and functional modification of many targets and can be widely applied towards the fabrication of complex biomaterials and future in vivo labeling and detection. AAAS 2018-12-12 /pmc/articles/PMC6750040/ /pubmed/31549027 http://dx.doi.org/10.1155/2018/3152870 Text en Copyright © 2018 Xianglong Hu et al. https://creativecommons.org/licenses/by/4.0/ Exclusive licensee Science and Technology Review Publishing House. Distributed under a Creative Commons Attribution License (CC BY 4.0). |
spellingShingle | Research Article Hu, Xianglong Zhao, Xueqian He, Benzhao Zhao, Zheng Zheng, Zheng Zhang, Pengfei Shi, Xiujuan Kwok, Ryan T. K. Lam, Jacky W. Y. Qin, Anjun Tang, Ben Zhong A Simple Approach to Bioconjugation at Diverse Levels: Metal-Free Click Reactions of Activated Alkynes with Native Groups of Biotargets without Prefunctionalization |
title | A Simple Approach to Bioconjugation at Diverse Levels: Metal-Free Click Reactions of Activated Alkynes with Native Groups of Biotargets without Prefunctionalization |
title_full | A Simple Approach to Bioconjugation at Diverse Levels: Metal-Free Click Reactions of Activated Alkynes with Native Groups of Biotargets without Prefunctionalization |
title_fullStr | A Simple Approach to Bioconjugation at Diverse Levels: Metal-Free Click Reactions of Activated Alkynes with Native Groups of Biotargets without Prefunctionalization |
title_full_unstemmed | A Simple Approach to Bioconjugation at Diverse Levels: Metal-Free Click Reactions of Activated Alkynes with Native Groups of Biotargets without Prefunctionalization |
title_short | A Simple Approach to Bioconjugation at Diverse Levels: Metal-Free Click Reactions of Activated Alkynes with Native Groups of Biotargets without Prefunctionalization |
title_sort | simple approach to bioconjugation at diverse levels: metal-free click reactions of activated alkynes with native groups of biotargets without prefunctionalization |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6750040/ https://www.ncbi.nlm.nih.gov/pubmed/31549027 http://dx.doi.org/10.1155/2018/3152870 |
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