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Nano–Bio Interactions of Extracellular Vesicles with Gold Nanoislands for Early Cancer Diagnosis

Extracellular vesicles or exosomes are membrane encapsulated biological nanometric particles secreted virtually by all types of cells throughout the animal kingdom. They carry a cargo of active molecules to proximal and distal cells of the body as mechanism of physiological communication, to maintai...

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Detalles Bibliográficos
Autores principales: Bathini, S., Raju, D., Badilescu, S., Kumar, A., Ouellette, R. J., Ghosh, A., Packirisamy, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AAAS 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6750071/
https://www.ncbi.nlm.nih.gov/pubmed/31549028
http://dx.doi.org/10.1155/2018/3917986
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author Bathini, S.
Raju, D.
Badilescu, S.
Kumar, A.
Ouellette, R. J.
Ghosh, A.
Packirisamy, M.
author_facet Bathini, S.
Raju, D.
Badilescu, S.
Kumar, A.
Ouellette, R. J.
Ghosh, A.
Packirisamy, M.
author_sort Bathini, S.
collection PubMed
description Extracellular vesicles or exosomes are membrane encapsulated biological nanometric particles secreted virtually by all types of cells throughout the animal kingdom. They carry a cargo of active molecules to proximal and distal cells of the body as mechanism of physiological communication, to maintain natural homeostasis as well as pathological responses. Exosomes carry a tremendous potential for liquid biopsy and therapeutic applications. Thus, there is a global demand for simple and robust exosome isolation methods amenable to point-of-care diagnosis and quality control of therapeutic exosome manufacturing. This can be achieved by molecular profiling of the exosomes for use with specific sets of molecular-markers for diagnosis and quality control. Liquid biopsy is undoubtedly the most promising diagnosis process to advance “personalized medicine.” Currently, liquid biopsy is based on circulating cancer cells, cell free-DNA, or exosomes. Exosomes potentially provide promise for early-stage diagnostic possibility; in order to facilitate superior diagnosis and isolation of exosomes, a novel platform is developed to detect and capture them, based on localized surface plasmon resonance (LSPR) of gold nanoislands, through strong affinity between exosomes and peptide called Venceremin or Vn96. Physical modeling, based on the characteristics of the gold nanoislands and the bioentities involved in the sensing, is also developed to determine the detection capability of the platform, which is optimized experimentally at each stage. Preliminary results and modeling present a relationship between the plasmonic shift and the concentration of exosomes and, essentially, indicate possibilities for label-free early diagnosis.
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spelling pubmed-67500712019-09-23 Nano–Bio Interactions of Extracellular Vesicles with Gold Nanoislands for Early Cancer Diagnosis Bathini, S. Raju, D. Badilescu, S. Kumar, A. Ouellette, R. J. Ghosh, A. Packirisamy, M. Research (Wash D C) Research Article Extracellular vesicles or exosomes are membrane encapsulated biological nanometric particles secreted virtually by all types of cells throughout the animal kingdom. They carry a cargo of active molecules to proximal and distal cells of the body as mechanism of physiological communication, to maintain natural homeostasis as well as pathological responses. Exosomes carry a tremendous potential for liquid biopsy and therapeutic applications. Thus, there is a global demand for simple and robust exosome isolation methods amenable to point-of-care diagnosis and quality control of therapeutic exosome manufacturing. This can be achieved by molecular profiling of the exosomes for use with specific sets of molecular-markers for diagnosis and quality control. Liquid biopsy is undoubtedly the most promising diagnosis process to advance “personalized medicine.” Currently, liquid biopsy is based on circulating cancer cells, cell free-DNA, or exosomes. Exosomes potentially provide promise for early-stage diagnostic possibility; in order to facilitate superior diagnosis and isolation of exosomes, a novel platform is developed to detect and capture them, based on localized surface plasmon resonance (LSPR) of gold nanoislands, through strong affinity between exosomes and peptide called Venceremin or Vn96. Physical modeling, based on the characteristics of the gold nanoislands and the bioentities involved in the sensing, is also developed to determine the detection capability of the platform, which is optimized experimentally at each stage. Preliminary results and modeling present a relationship between the plasmonic shift and the concentration of exosomes and, essentially, indicate possibilities for label-free early diagnosis. AAAS 2018-10-09 /pmc/articles/PMC6750071/ /pubmed/31549028 http://dx.doi.org/10.1155/2018/3917986 Text en Copyright © 2018 S. Bathini et al. https://creativecommons.org/licenses/by/4.0/ Exclusive licensee Science and Technology Review Publishing House. Distributed under a Creative Commons Attribution License (CC BY 4.0).
spellingShingle Research Article
Bathini, S.
Raju, D.
Badilescu, S.
Kumar, A.
Ouellette, R. J.
Ghosh, A.
Packirisamy, M.
Nano–Bio Interactions of Extracellular Vesicles with Gold Nanoislands for Early Cancer Diagnosis
title Nano–Bio Interactions of Extracellular Vesicles with Gold Nanoislands for Early Cancer Diagnosis
title_full Nano–Bio Interactions of Extracellular Vesicles with Gold Nanoislands for Early Cancer Diagnosis
title_fullStr Nano–Bio Interactions of Extracellular Vesicles with Gold Nanoislands for Early Cancer Diagnosis
title_full_unstemmed Nano–Bio Interactions of Extracellular Vesicles with Gold Nanoislands for Early Cancer Diagnosis
title_short Nano–Bio Interactions of Extracellular Vesicles with Gold Nanoislands for Early Cancer Diagnosis
title_sort nano–bio interactions of extracellular vesicles with gold nanoislands for early cancer diagnosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6750071/
https://www.ncbi.nlm.nih.gov/pubmed/31549028
http://dx.doi.org/10.1155/2018/3917986
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