Cargando…
Protoporphyrin IX-loaded laminarin nanoparticles for anticancer treatment, their cellular behavior, ROS detection, and animal studies
Laminarin conjugate-based nano-scaled particles were in this study proposed as a delivery system for protoporphyrin IX (Pp IX) in photodynamic therapy (PDT) of human breast cancer cells (MCF-7). Hematin-Laminarin-Dithiodipropionic Acid-MGK, named as HLDM, was an amphiphilic carrier material with dua...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751237/ https://www.ncbi.nlm.nih.gov/pubmed/31535237 http://dx.doi.org/10.1186/s11671-019-3138-0 |
_version_ | 1783452580298358784 |
---|---|
author | Yu, Yueming Wang, Bingjie Guo, Chunjing Zhao, Feng Chen, Daquan |
author_facet | Yu, Yueming Wang, Bingjie Guo, Chunjing Zhao, Feng Chen, Daquan |
author_sort | Yu, Yueming |
collection | PubMed |
description | Laminarin conjugate-based nano-scaled particles were in this study proposed as a delivery system for protoporphyrin IX (Pp IX) in photodynamic therapy (PDT) of human breast cancer cells (MCF-7). Hematin-Laminarin-Dithiodipropionic Acid-MGK, named as HLDM, was an amphiphilic carrier material with dual pH/redox sensitive that could be used to load hydrophobic drug to improve their solubility and enhance biocompatibility. Therefore, we combined photosensitizer (Pp IX) with HLDM to fabricate a novel nano-micelles, herein called Pp IX-loaded HLDM micelles. The Pp IX-loaded HLDM micelles were 149.3 ± 35 nm sized in neutral water. Phototoxicity, in vitro PDT effect, and dual sensibility to pH and redox microenvironment of Pp IX-loaded HLDM micelles were examined at different concentrations by using MCF-7 human breast cancer cells. The experiments on phototoxicity and reactive oxygen species (ROS) production proved that the micelles could produce PDT to kill the cancer cells with a certain wavelength light. The apoptosis experiment indicated that the micelles could cause nuclear damage. In vivo PDT effect of the micelles was studied by constructing the tumor-bearing nude mouse model of MCF-7 cells. In vivo studies showed that the Pp IX-loaded HLDM micelles could induce remarkable anti-tumor effect. A promising laminarin-based nanomedicine platform acts as a new drug delivery system to enhance the uptake, accumulation, and PDT efficacy of Pp IX in vitro and in vivo. |
format | Online Article Text |
id | pubmed-6751237 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-67512372019-10-04 Protoporphyrin IX-loaded laminarin nanoparticles for anticancer treatment, their cellular behavior, ROS detection, and animal studies Yu, Yueming Wang, Bingjie Guo, Chunjing Zhao, Feng Chen, Daquan Nanoscale Res Lett Nano Express Laminarin conjugate-based nano-scaled particles were in this study proposed as a delivery system for protoporphyrin IX (Pp IX) in photodynamic therapy (PDT) of human breast cancer cells (MCF-7). Hematin-Laminarin-Dithiodipropionic Acid-MGK, named as HLDM, was an amphiphilic carrier material with dual pH/redox sensitive that could be used to load hydrophobic drug to improve their solubility and enhance biocompatibility. Therefore, we combined photosensitizer (Pp IX) with HLDM to fabricate a novel nano-micelles, herein called Pp IX-loaded HLDM micelles. The Pp IX-loaded HLDM micelles were 149.3 ± 35 nm sized in neutral water. Phototoxicity, in vitro PDT effect, and dual sensibility to pH and redox microenvironment of Pp IX-loaded HLDM micelles were examined at different concentrations by using MCF-7 human breast cancer cells. The experiments on phototoxicity and reactive oxygen species (ROS) production proved that the micelles could produce PDT to kill the cancer cells with a certain wavelength light. The apoptosis experiment indicated that the micelles could cause nuclear damage. In vivo PDT effect of the micelles was studied by constructing the tumor-bearing nude mouse model of MCF-7 cells. In vivo studies showed that the Pp IX-loaded HLDM micelles could induce remarkable anti-tumor effect. A promising laminarin-based nanomedicine platform acts as a new drug delivery system to enhance the uptake, accumulation, and PDT efficacy of Pp IX in vitro and in vivo. Springer US 2019-09-18 /pmc/articles/PMC6751237/ /pubmed/31535237 http://dx.doi.org/10.1186/s11671-019-3138-0 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Nano Express Yu, Yueming Wang, Bingjie Guo, Chunjing Zhao, Feng Chen, Daquan Protoporphyrin IX-loaded laminarin nanoparticles for anticancer treatment, their cellular behavior, ROS detection, and animal studies |
title | Protoporphyrin IX-loaded laminarin nanoparticles for anticancer treatment, their cellular behavior, ROS detection, and animal studies |
title_full | Protoporphyrin IX-loaded laminarin nanoparticles for anticancer treatment, their cellular behavior, ROS detection, and animal studies |
title_fullStr | Protoporphyrin IX-loaded laminarin nanoparticles for anticancer treatment, their cellular behavior, ROS detection, and animal studies |
title_full_unstemmed | Protoporphyrin IX-loaded laminarin nanoparticles for anticancer treatment, their cellular behavior, ROS detection, and animal studies |
title_short | Protoporphyrin IX-loaded laminarin nanoparticles for anticancer treatment, their cellular behavior, ROS detection, and animal studies |
title_sort | protoporphyrin ix-loaded laminarin nanoparticles for anticancer treatment, their cellular behavior, ros detection, and animal studies |
topic | Nano Express |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751237/ https://www.ncbi.nlm.nih.gov/pubmed/31535237 http://dx.doi.org/10.1186/s11671-019-3138-0 |
work_keys_str_mv | AT yuyueming protoporphyrinixloadedlaminarinnanoparticlesforanticancertreatmenttheircellularbehaviorrosdetectionandanimalstudies AT wangbingjie protoporphyrinixloadedlaminarinnanoparticlesforanticancertreatmenttheircellularbehaviorrosdetectionandanimalstudies AT guochunjing protoporphyrinixloadedlaminarinnanoparticlesforanticancertreatmenttheircellularbehaviorrosdetectionandanimalstudies AT zhaofeng protoporphyrinixloadedlaminarinnanoparticlesforanticancertreatmenttheircellularbehaviorrosdetectionandanimalstudies AT chendaquan protoporphyrinixloadedlaminarinnanoparticlesforanticancertreatmenttheircellularbehaviorrosdetectionandanimalstudies |