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The first fungal laccase with an alkaline pH optimum obtained by directed evolution and its application in indigo dye decolorization

Engineering of fungal laccases with optimum catalytic activity at alkaline pH has been a long-lasting challenge. In this study, a mutant library containing 3000 clones was obtained by error-prone PCR to adapt the optimum pH of a fungal laccase Lcc9 from the basidiomycete Coprinopsis cinerea. After t...

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Detalles Bibliográficos
Autores principales:	Yin, Qiang, Zhou, Gang, Peng, Can, Zhang, Yinliang, Kües, Ursula, Liu, Juanjuan, Xiao, Yazhong, Fang, Zemin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2019
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751238/ https://www.ncbi.nlm.nih.gov/pubmed/31535295 http://dx.doi.org/10.1186/s13568-019-0878-2

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