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Production of the herb Ruta chalepensis L. expressing amyloid β-GFP fusion protein

The herb Ruta chalepensis L. exhibits medical effects, such as anti-inflammatory, central nervous system depressant, and antipyretic activities. However, a genetic transformation method has not yet been developed for this species. In this paper, a simple and efficient tissue culture and genetic tran...

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Detalles Bibliográficos
Autores principales: YOSHIDA, Taiji, WATANABE, Yuichiro, ISHIURA, Shoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japan Academy 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751298/
https://www.ncbi.nlm.nih.gov/pubmed/31189782
http://dx.doi.org/10.2183/pjab.95.021
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author YOSHIDA, Taiji
WATANABE, Yuichiro
ISHIURA, Shoichi
author_facet YOSHIDA, Taiji
WATANABE, Yuichiro
ISHIURA, Shoichi
author_sort YOSHIDA, Taiji
collection PubMed
description The herb Ruta chalepensis L. exhibits medical effects, such as anti-inflammatory, central nervous system depressant, and antipyretic activities. However, a genetic transformation method has not yet been developed for this species. In this paper, a simple and efficient tissue culture and genetic transformation system for R. chalepensis is reported. An amyloid β-peptide (Aβ) gene, which is considered to be a causative agent of Alzheimer’s disease (AD), fused with green-fluorescent protein (GFP), was introduced into R. chalepensis. When the leaves of R. chalepensis expressing Aβ-GFP were administered orally to C57BL/6J mice, serum anti-Aβ antibody titers of several mice were elevated without the use of an adjuvant. These results indicated that an oral vaccine against AD using R. chalepensis may be feasible. R. chalepensis is rich in bioactive compounds that may have synergistic effects with the vaccine for AD. Plant-derived vaccines are safer and cheaper than those produced from animal cells or microbes, because plants can serve as biofactories at low cost and with high biosynthetic capacity.
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spelling pubmed-67512982019-09-25 Production of the herb Ruta chalepensis L. expressing amyloid β-GFP fusion protein YOSHIDA, Taiji WATANABE, Yuichiro ISHIURA, Shoichi Proc Jpn Acad Ser B Phys Biol Sci Original Article The herb Ruta chalepensis L. exhibits medical effects, such as anti-inflammatory, central nervous system depressant, and antipyretic activities. However, a genetic transformation method has not yet been developed for this species. In this paper, a simple and efficient tissue culture and genetic transformation system for R. chalepensis is reported. An amyloid β-peptide (Aβ) gene, which is considered to be a causative agent of Alzheimer’s disease (AD), fused with green-fluorescent protein (GFP), was introduced into R. chalepensis. When the leaves of R. chalepensis expressing Aβ-GFP were administered orally to C57BL/6J mice, serum anti-Aβ antibody titers of several mice were elevated without the use of an adjuvant. These results indicated that an oral vaccine against AD using R. chalepensis may be feasible. R. chalepensis is rich in bioactive compounds that may have synergistic effects with the vaccine for AD. Plant-derived vaccines are safer and cheaper than those produced from animal cells or microbes, because plants can serve as biofactories at low cost and with high biosynthetic capacity. The Japan Academy 2019-06-11 /pmc/articles/PMC6751298/ /pubmed/31189782 http://dx.doi.org/10.2183/pjab.95.021 Text en © 2019 The Japan Academy This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
YOSHIDA, Taiji
WATANABE, Yuichiro
ISHIURA, Shoichi
Production of the herb Ruta chalepensis L. expressing amyloid β-GFP fusion protein
title Production of the herb Ruta chalepensis L. expressing amyloid β-GFP fusion protein
title_full Production of the herb Ruta chalepensis L. expressing amyloid β-GFP fusion protein
title_fullStr Production of the herb Ruta chalepensis L. expressing amyloid β-GFP fusion protein
title_full_unstemmed Production of the herb Ruta chalepensis L. expressing amyloid β-GFP fusion protein
title_short Production of the herb Ruta chalepensis L. expressing amyloid β-GFP fusion protein
title_sort production of the herb ruta chalepensis l. expressing amyloid β-gfp fusion protein
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751298/
https://www.ncbi.nlm.nih.gov/pubmed/31189782
http://dx.doi.org/10.2183/pjab.95.021
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