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Photopheresis efficacy in the treatment of rheumatoid arthritis: a pre-clinical proof of concept
BACKGROUND: Despite major advances in rheumatoid arthritis outcome, not all patients achieve remission, and there is still an unmet need for new therapeutic approaches. This study aimed at evaluating in a pre-clinical murine model the efficacy of extracorporeal photopheresis (ECP) in the treatment o...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751641/ https://www.ncbi.nlm.nih.gov/pubmed/31533744 http://dx.doi.org/10.1186/s12967-019-2066-1 |
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author | Coppard, Céline Bonnefoy, Francis Hannani, Dalil Gabert, Françoise Manches, Olivier Plumas, Joel Perruche, Sylvain Chaperot, Laurence |
author_facet | Coppard, Céline Bonnefoy, Francis Hannani, Dalil Gabert, Françoise Manches, Olivier Plumas, Joel Perruche, Sylvain Chaperot, Laurence |
author_sort | Coppard, Céline |
collection | PubMed |
description | BACKGROUND: Despite major advances in rheumatoid arthritis outcome, not all patients achieve remission, and there is still an unmet need for new therapeutic approaches. This study aimed at evaluating in a pre-clinical murine model the efficacy of extracorporeal photopheresis (ECP) in the treatment of rheumatoid arthritis, and to provide a relevant study model for dissecting ECP mechanism of action in autoimmune diseases. METHODS: DBA/1 mice were immunized by subcutaneous injection of bovine collagen type II, in order to initiate the development of collagen-induced arthritis (CIA). Arthritic mice received 3 ECP treatments every other day, with psoralen + UVA-treated (PUVA) spleen cells obtained from arthritic mice. Arthritis score was measured, and immune cell subsets were monitored. RESULTS: ECP-treated mice recovered from arthritis as evidenced by a decreasing arthritic score over time. Significant decrease in the frequency of Th17 cells in the spleen of treated mice was observed. Interestingly, while PUVA-treated spleen cells from healthy mouse had no effect, PUVA-treated arthritic mouse derived-spleen cells were able to induce control of arthritis development. CONCLUSIONS: Our results demonstrate that ECP can control arthritis in CIA-mice, and clarifies ECP mechanisms of action, showing ECP efficacy and Th17 decrease only when arthritogenic T cells are contained within the treated sample. These data represent a pre-clinical proof of concept supporting the use of ECP in the treatment of RA in Human. |
format | Online Article Text |
id | pubmed-6751641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-67516412019-09-23 Photopheresis efficacy in the treatment of rheumatoid arthritis: a pre-clinical proof of concept Coppard, Céline Bonnefoy, Francis Hannani, Dalil Gabert, Françoise Manches, Olivier Plumas, Joel Perruche, Sylvain Chaperot, Laurence J Transl Med Research BACKGROUND: Despite major advances in rheumatoid arthritis outcome, not all patients achieve remission, and there is still an unmet need for new therapeutic approaches. This study aimed at evaluating in a pre-clinical murine model the efficacy of extracorporeal photopheresis (ECP) in the treatment of rheumatoid arthritis, and to provide a relevant study model for dissecting ECP mechanism of action in autoimmune diseases. METHODS: DBA/1 mice were immunized by subcutaneous injection of bovine collagen type II, in order to initiate the development of collagen-induced arthritis (CIA). Arthritic mice received 3 ECP treatments every other day, with psoralen + UVA-treated (PUVA) spleen cells obtained from arthritic mice. Arthritis score was measured, and immune cell subsets were monitored. RESULTS: ECP-treated mice recovered from arthritis as evidenced by a decreasing arthritic score over time. Significant decrease in the frequency of Th17 cells in the spleen of treated mice was observed. Interestingly, while PUVA-treated spleen cells from healthy mouse had no effect, PUVA-treated arthritic mouse derived-spleen cells were able to induce control of arthritis development. CONCLUSIONS: Our results demonstrate that ECP can control arthritis in CIA-mice, and clarifies ECP mechanisms of action, showing ECP efficacy and Th17 decrease only when arthritogenic T cells are contained within the treated sample. These data represent a pre-clinical proof of concept supporting the use of ECP in the treatment of RA in Human. BioMed Central 2019-09-18 /pmc/articles/PMC6751641/ /pubmed/31533744 http://dx.doi.org/10.1186/s12967-019-2066-1 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Coppard, Céline Bonnefoy, Francis Hannani, Dalil Gabert, Françoise Manches, Olivier Plumas, Joel Perruche, Sylvain Chaperot, Laurence Photopheresis efficacy in the treatment of rheumatoid arthritis: a pre-clinical proof of concept |
title | Photopheresis efficacy in the treatment of rheumatoid arthritis: a pre-clinical proof of concept |
title_full | Photopheresis efficacy in the treatment of rheumatoid arthritis: a pre-clinical proof of concept |
title_fullStr | Photopheresis efficacy in the treatment of rheumatoid arthritis: a pre-clinical proof of concept |
title_full_unstemmed | Photopheresis efficacy in the treatment of rheumatoid arthritis: a pre-clinical proof of concept |
title_short | Photopheresis efficacy in the treatment of rheumatoid arthritis: a pre-clinical proof of concept |
title_sort | photopheresis efficacy in the treatment of rheumatoid arthritis: a pre-clinical proof of concept |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751641/ https://www.ncbi.nlm.nih.gov/pubmed/31533744 http://dx.doi.org/10.1186/s12967-019-2066-1 |
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