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Virulence structure and its genetic diversity analyses of Blumeria graminis f. sp. tritici isolates in China

BACKGROUND: Blumeria graminis f. sp. tritici (Bgt), the causal agent of wheat powdery mildew severely affects yield security wheat production in China. Understanding the virulence structure and genetic variations of this pathogen is important for breeding wheat lines resistant to wheat powdery milde...

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Autores principales: Wu, Xian Xin, Xu, Xiao Feng, Ma, De Xin, Chen, Rong Zhen, Li, Tian Ya, Cao, Yuan Yin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751876/
https://www.ncbi.nlm.nih.gov/pubmed/31533625
http://dx.doi.org/10.1186/s12862-019-1511-3
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author Wu, Xian Xin
Xu, Xiao Feng
Ma, De Xin
Chen, Rong Zhen
Li, Tian Ya
Cao, Yuan Yin
author_facet Wu, Xian Xin
Xu, Xiao Feng
Ma, De Xin
Chen, Rong Zhen
Li, Tian Ya
Cao, Yuan Yin
author_sort Wu, Xian Xin
collection PubMed
description BACKGROUND: Blumeria graminis f. sp. tritici (Bgt), the causal agent of wheat powdery mildew severely affects yield security wheat production in China. Understanding the virulence structure and genetic variations of this pathogen is important for breeding wheat lines resistant to wheat powdery mildew. However, information related to genes controlling resistance remains elusive. This study analyzes the virulence structure and the genetic diversity of pathogenic Bgt populations isolated from northeastern (Liaoning, Heilongjiang) and northwestern (Gansu) China, two representative wheat producing areas, on 37 wheat cultivars each carrying a known powdery mildew resistance (Pm) gene. RESULTS: Bgt isolates from northeastern China show higher frequencies of virulence genes than populations from Gansu Province. Many of the known Pm genes failed to provide resistance in this study. However, Pm21 provided 100% resistance to all isolates from all three provinces, obtained during two consecutive years, while Pm13 provided 100% resistance in Gansu. Pm13, Pm16, Pm18, and Pm22 also showed partial resistance in northeastern China, while Pm16, Pm18, Pm22, Pm5 + 6 and Pm2 + 6 +? maintained some resistance in Gansu. Genetic diversity among populations in different regions was detected by cluster analyses using expressed sequence tag-simple sequence repeat (EST-SSR). When the genetic similarity coefficient is relatively high, populations from the same regional origin are mostly clustered into one group while populations from different regions exhibit large genetic differences. CONCLUSION: Pm21 remains the best choice for breeding programs to maintain resistance to Bgt. Only 58% of the isolates tested show a clear correlation between EST-SSR genetic polymorphisms and frequency of virulence gene data.
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spelling pubmed-67518762019-09-23 Virulence structure and its genetic diversity analyses of Blumeria graminis f. sp. tritici isolates in China Wu, Xian Xin Xu, Xiao Feng Ma, De Xin Chen, Rong Zhen Li, Tian Ya Cao, Yuan Yin BMC Evol Biol Research Article BACKGROUND: Blumeria graminis f. sp. tritici (Bgt), the causal agent of wheat powdery mildew severely affects yield security wheat production in China. Understanding the virulence structure and genetic variations of this pathogen is important for breeding wheat lines resistant to wheat powdery mildew. However, information related to genes controlling resistance remains elusive. This study analyzes the virulence structure and the genetic diversity of pathogenic Bgt populations isolated from northeastern (Liaoning, Heilongjiang) and northwestern (Gansu) China, two representative wheat producing areas, on 37 wheat cultivars each carrying a known powdery mildew resistance (Pm) gene. RESULTS: Bgt isolates from northeastern China show higher frequencies of virulence genes than populations from Gansu Province. Many of the known Pm genes failed to provide resistance in this study. However, Pm21 provided 100% resistance to all isolates from all three provinces, obtained during two consecutive years, while Pm13 provided 100% resistance in Gansu. Pm13, Pm16, Pm18, and Pm22 also showed partial resistance in northeastern China, while Pm16, Pm18, Pm22, Pm5 + 6 and Pm2 + 6 +? maintained some resistance in Gansu. Genetic diversity among populations in different regions was detected by cluster analyses using expressed sequence tag-simple sequence repeat (EST-SSR). When the genetic similarity coefficient is relatively high, populations from the same regional origin are mostly clustered into one group while populations from different regions exhibit large genetic differences. CONCLUSION: Pm21 remains the best choice for breeding programs to maintain resistance to Bgt. Only 58% of the isolates tested show a clear correlation between EST-SSR genetic polymorphisms and frequency of virulence gene data. BioMed Central 2019-09-18 /pmc/articles/PMC6751876/ /pubmed/31533625 http://dx.doi.org/10.1186/s12862-019-1511-3 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wu, Xian Xin
Xu, Xiao Feng
Ma, De Xin
Chen, Rong Zhen
Li, Tian Ya
Cao, Yuan Yin
Virulence structure and its genetic diversity analyses of Blumeria graminis f. sp. tritici isolates in China
title Virulence structure and its genetic diversity analyses of Blumeria graminis f. sp. tritici isolates in China
title_full Virulence structure and its genetic diversity analyses of Blumeria graminis f. sp. tritici isolates in China
title_fullStr Virulence structure and its genetic diversity analyses of Blumeria graminis f. sp. tritici isolates in China
title_full_unstemmed Virulence structure and its genetic diversity analyses of Blumeria graminis f. sp. tritici isolates in China
title_short Virulence structure and its genetic diversity analyses of Blumeria graminis f. sp. tritici isolates in China
title_sort virulence structure and its genetic diversity analyses of blumeria graminis f. sp. tritici isolates in china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751876/
https://www.ncbi.nlm.nih.gov/pubmed/31533625
http://dx.doi.org/10.1186/s12862-019-1511-3
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