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Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology
BACKGROUND: Research on microparticles is rapidly evolving and has extended to the field of many diseases. It is unclear whether microparticles can be stored frozen. In this study, our goal was to verify whether cryopreservation had an effect on the properties of the microparticles. MATERIAL/METHODS...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6752109/ https://www.ncbi.nlm.nih.gov/pubmed/31488807 http://dx.doi.org/10.12659/MSM.917962 |
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author | Dong, Xinlong Li, Mengqi Li, Qifeng Gao, Yalong Liu, Li Chen, Xin Zhou, Ziwei Rong, Hongtao Zhang, Jianning Tian, Ye |
author_facet | Dong, Xinlong Li, Mengqi Li, Qifeng Gao, Yalong Liu, Li Chen, Xin Zhou, Ziwei Rong, Hongtao Zhang, Jianning Tian, Ye |
author_sort | Dong, Xinlong |
collection | PubMed |
description | BACKGROUND: Research on microparticles is rapidly evolving and has extended to the field of many diseases. It is unclear whether microparticles can be stored frozen. In this study, our goal was to verify whether cryopreservation had an effect on the properties of the microparticles. MATERIAL/METHODS: We obtained C57BL/6J mouse-derived microparticles by grinding and gradient centrifugation. The specimens were divided into 2 groups: without dimethyl sulfoxide and with dimethyl sulfoxide. The microparticles were then stored at 25°C, 4°C, −20°C, −80°C, and −196°C for 0.5 days, 1 day, 3 days, 5 days, and 7 days. We tested whether the concentration, coagulation function, diameter distribution, and morphology of the microparticles in the 2 groups changed compared to those of a fresh sample. RESULTS: We discovered that the concentrations of total microparticles, annexin V-positive microparticles, and brain-derived microparticles changed with freezing. The coagulation function, morphology, and size distribution of the microparticles were also affected by cryopreservation. Finally, there was no difference in the effects of cryopreservation on microparticles between the dimethyl sulfoxide group and the dimethyl sulfoxide-free group. CONCLUSIONS: This study suggests that cryopreservation has diverse effects on microparticles within 1 week and that dimethyl sulfoxide has no protective effect on cryopreserved microparticles. Therefore, microparticles should be used fresh for future studies, and they should not be cryopreserved with or without dimethyl sulfoxide. |
format | Online Article Text |
id | pubmed-6752109 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67521092019-09-20 Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology Dong, Xinlong Li, Mengqi Li, Qifeng Gao, Yalong Liu, Li Chen, Xin Zhou, Ziwei Rong, Hongtao Zhang, Jianning Tian, Ye Med Sci Monit Animal Study BACKGROUND: Research on microparticles is rapidly evolving and has extended to the field of many diseases. It is unclear whether microparticles can be stored frozen. In this study, our goal was to verify whether cryopreservation had an effect on the properties of the microparticles. MATERIAL/METHODS: We obtained C57BL/6J mouse-derived microparticles by grinding and gradient centrifugation. The specimens were divided into 2 groups: without dimethyl sulfoxide and with dimethyl sulfoxide. The microparticles were then stored at 25°C, 4°C, −20°C, −80°C, and −196°C for 0.5 days, 1 day, 3 days, 5 days, and 7 days. We tested whether the concentration, coagulation function, diameter distribution, and morphology of the microparticles in the 2 groups changed compared to those of a fresh sample. RESULTS: We discovered that the concentrations of total microparticles, annexin V-positive microparticles, and brain-derived microparticles changed with freezing. The coagulation function, morphology, and size distribution of the microparticles were also affected by cryopreservation. Finally, there was no difference in the effects of cryopreservation on microparticles between the dimethyl sulfoxide group and the dimethyl sulfoxide-free group. CONCLUSIONS: This study suggests that cryopreservation has diverse effects on microparticles within 1 week and that dimethyl sulfoxide has no protective effect on cryopreserved microparticles. Therefore, microparticles should be used fresh for future studies, and they should not be cryopreserved with or without dimethyl sulfoxide. International Scientific Literature, Inc. 2019-09-06 /pmc/articles/PMC6752109/ /pubmed/31488807 http://dx.doi.org/10.12659/MSM.917962 Text en © Med Sci Monit, 2019 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Animal Study Dong, Xinlong Li, Mengqi Li, Qifeng Gao, Yalong Liu, Li Chen, Xin Zhou, Ziwei Rong, Hongtao Zhang, Jianning Tian, Ye Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology |
title | Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology |
title_full | Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology |
title_fullStr | Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology |
title_full_unstemmed | Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology |
title_short | Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology |
title_sort | effects of cryopreservation on microparticles concentration, procoagulant function, size distribution, and morphology |
topic | Animal Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6752109/ https://www.ncbi.nlm.nih.gov/pubmed/31488807 http://dx.doi.org/10.12659/MSM.917962 |
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