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Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology

BACKGROUND: Research on microparticles is rapidly evolving and has extended to the field of many diseases. It is unclear whether microparticles can be stored frozen. In this study, our goal was to verify whether cryopreservation had an effect on the properties of the microparticles. MATERIAL/METHODS...

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Autores principales: Dong, Xinlong, Li, Mengqi, Li, Qifeng, Gao, Yalong, Liu, Li, Chen, Xin, Zhou, Ziwei, Rong, Hongtao, Zhang, Jianning, Tian, Ye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6752109/
https://www.ncbi.nlm.nih.gov/pubmed/31488807
http://dx.doi.org/10.12659/MSM.917962
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author Dong, Xinlong
Li, Mengqi
Li, Qifeng
Gao, Yalong
Liu, Li
Chen, Xin
Zhou, Ziwei
Rong, Hongtao
Zhang, Jianning
Tian, Ye
author_facet Dong, Xinlong
Li, Mengqi
Li, Qifeng
Gao, Yalong
Liu, Li
Chen, Xin
Zhou, Ziwei
Rong, Hongtao
Zhang, Jianning
Tian, Ye
author_sort Dong, Xinlong
collection PubMed
description BACKGROUND: Research on microparticles is rapidly evolving and has extended to the field of many diseases. It is unclear whether microparticles can be stored frozen. In this study, our goal was to verify whether cryopreservation had an effect on the properties of the microparticles. MATERIAL/METHODS: We obtained C57BL/6J mouse-derived microparticles by grinding and gradient centrifugation. The specimens were divided into 2 groups: without dimethyl sulfoxide and with dimethyl sulfoxide. The microparticles were then stored at 25°C, 4°C, −20°C, −80°C, and −196°C for 0.5 days, 1 day, 3 days, 5 days, and 7 days. We tested whether the concentration, coagulation function, diameter distribution, and morphology of the microparticles in the 2 groups changed compared to those of a fresh sample. RESULTS: We discovered that the concentrations of total microparticles, annexin V-positive microparticles, and brain-derived microparticles changed with freezing. The coagulation function, morphology, and size distribution of the microparticles were also affected by cryopreservation. Finally, there was no difference in the effects of cryopreservation on microparticles between the dimethyl sulfoxide group and the dimethyl sulfoxide-free group. CONCLUSIONS: This study suggests that cryopreservation has diverse effects on microparticles within 1 week and that dimethyl sulfoxide has no protective effect on cryopreserved microparticles. Therefore, microparticles should be used fresh for future studies, and they should not be cryopreserved with or without dimethyl sulfoxide.
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spelling pubmed-67521092019-09-20 Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology Dong, Xinlong Li, Mengqi Li, Qifeng Gao, Yalong Liu, Li Chen, Xin Zhou, Ziwei Rong, Hongtao Zhang, Jianning Tian, Ye Med Sci Monit Animal Study BACKGROUND: Research on microparticles is rapidly evolving and has extended to the field of many diseases. It is unclear whether microparticles can be stored frozen. In this study, our goal was to verify whether cryopreservation had an effect on the properties of the microparticles. MATERIAL/METHODS: We obtained C57BL/6J mouse-derived microparticles by grinding and gradient centrifugation. The specimens were divided into 2 groups: without dimethyl sulfoxide and with dimethyl sulfoxide. The microparticles were then stored at 25°C, 4°C, −20°C, −80°C, and −196°C for 0.5 days, 1 day, 3 days, 5 days, and 7 days. We tested whether the concentration, coagulation function, diameter distribution, and morphology of the microparticles in the 2 groups changed compared to those of a fresh sample. RESULTS: We discovered that the concentrations of total microparticles, annexin V-positive microparticles, and brain-derived microparticles changed with freezing. The coagulation function, morphology, and size distribution of the microparticles were also affected by cryopreservation. Finally, there was no difference in the effects of cryopreservation on microparticles between the dimethyl sulfoxide group and the dimethyl sulfoxide-free group. CONCLUSIONS: This study suggests that cryopreservation has diverse effects on microparticles within 1 week and that dimethyl sulfoxide has no protective effect on cryopreserved microparticles. Therefore, microparticles should be used fresh for future studies, and they should not be cryopreserved with or without dimethyl sulfoxide. International Scientific Literature, Inc. 2019-09-06 /pmc/articles/PMC6752109/ /pubmed/31488807 http://dx.doi.org/10.12659/MSM.917962 Text en © Med Sci Monit, 2019 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Animal Study
Dong, Xinlong
Li, Mengqi
Li, Qifeng
Gao, Yalong
Liu, Li
Chen, Xin
Zhou, Ziwei
Rong, Hongtao
Zhang, Jianning
Tian, Ye
Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology
title Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology
title_full Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology
title_fullStr Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology
title_full_unstemmed Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology
title_short Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology
title_sort effects of cryopreservation on microparticles concentration, procoagulant function, size distribution, and morphology
topic Animal Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6752109/
https://www.ncbi.nlm.nih.gov/pubmed/31488807
http://dx.doi.org/10.12659/MSM.917962
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