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A robust human norovirus replication model in zebrafish larvae

Human noroviruses (HuNoVs) are the most common cause of foodborne illness, with a societal cost of $60 billion and 219,000 deaths/year. The lack of robust small animal models has significantly hindered the understanding of norovirus biology and the development of effective therapeutics. Here we repo...

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Detalles Bibliográficos
Autores principales: Van Dycke, Jana, Ny, Annelii, Conceição-Neto, Nádia, Maes, Jan, Hosmillo, Myra, Cuvry, Arno, Goodfellow, Ian, Nogueira, Tatiane C., Verbeken, Erik, Matthijnssens, Jelle, de Witte, Peter, Neyts, Johan, Rocha-Pereira, Joana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6752765/
https://www.ncbi.nlm.nih.gov/pubmed/31536612
http://dx.doi.org/10.1371/journal.ppat.1008009
Descripción
Sumario:Human noroviruses (HuNoVs) are the most common cause of foodborne illness, with a societal cost of $60 billion and 219,000 deaths/year. The lack of robust small animal models has significantly hindered the understanding of norovirus biology and the development of effective therapeutics. Here we report that HuNoV GI and GII replicate to high titers in zebrafish (Danio rerio) larvae; replication peaks at day 2 post infection and is detectable for at least 6 days. The virus (HuNoV GII.4) could be passaged from larva to larva two consecutive times. HuNoV is detected in cells of the hematopoietic lineage and the intestine, supporting the notion of a dual tropism. Antiviral treatment reduces HuNoV replication by >2 log(10), showing that this model is suited for antiviral studies. Zebrafish larvae constitute a simple and robust replication model that will largely facilitate studies of HuNoV biology and the development of antiviral strategies.