Cargando…

A robust human norovirus replication model in zebrafish larvae

Human noroviruses (HuNoVs) are the most common cause of foodborne illness, with a societal cost of $60 billion and 219,000 deaths/year. The lack of robust small animal models has significantly hindered the understanding of norovirus biology and the development of effective therapeutics. Here we repo...

Descripción completa

Detalles Bibliográficos
Autores principales: Van Dycke, Jana, Ny, Annelii, Conceição-Neto, Nádia, Maes, Jan, Hosmillo, Myra, Cuvry, Arno, Goodfellow, Ian, Nogueira, Tatiane C., Verbeken, Erik, Matthijnssens, Jelle, de Witte, Peter, Neyts, Johan, Rocha-Pereira, Joana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6752765/
https://www.ncbi.nlm.nih.gov/pubmed/31536612
http://dx.doi.org/10.1371/journal.ppat.1008009
_version_ 1783452780549111808
author Van Dycke, Jana
Ny, Annelii
Conceição-Neto, Nádia
Maes, Jan
Hosmillo, Myra
Cuvry, Arno
Goodfellow, Ian
Nogueira, Tatiane C.
Verbeken, Erik
Matthijnssens, Jelle
de Witte, Peter
Neyts, Johan
Rocha-Pereira, Joana
author_facet Van Dycke, Jana
Ny, Annelii
Conceição-Neto, Nádia
Maes, Jan
Hosmillo, Myra
Cuvry, Arno
Goodfellow, Ian
Nogueira, Tatiane C.
Verbeken, Erik
Matthijnssens, Jelle
de Witte, Peter
Neyts, Johan
Rocha-Pereira, Joana
author_sort Van Dycke, Jana
collection PubMed
description Human noroviruses (HuNoVs) are the most common cause of foodborne illness, with a societal cost of $60 billion and 219,000 deaths/year. The lack of robust small animal models has significantly hindered the understanding of norovirus biology and the development of effective therapeutics. Here we report that HuNoV GI and GII replicate to high titers in zebrafish (Danio rerio) larvae; replication peaks at day 2 post infection and is detectable for at least 6 days. The virus (HuNoV GII.4) could be passaged from larva to larva two consecutive times. HuNoV is detected in cells of the hematopoietic lineage and the intestine, supporting the notion of a dual tropism. Antiviral treatment reduces HuNoV replication by >2 log(10), showing that this model is suited for antiviral studies. Zebrafish larvae constitute a simple and robust replication model that will largely facilitate studies of HuNoV biology and the development of antiviral strategies.
format Online
Article
Text
id pubmed-6752765
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-67527652019-09-27 A robust human norovirus replication model in zebrafish larvae Van Dycke, Jana Ny, Annelii Conceição-Neto, Nádia Maes, Jan Hosmillo, Myra Cuvry, Arno Goodfellow, Ian Nogueira, Tatiane C. Verbeken, Erik Matthijnssens, Jelle de Witte, Peter Neyts, Johan Rocha-Pereira, Joana PLoS Pathog Research Article Human noroviruses (HuNoVs) are the most common cause of foodborne illness, with a societal cost of $60 billion and 219,000 deaths/year. The lack of robust small animal models has significantly hindered the understanding of norovirus biology and the development of effective therapeutics. Here we report that HuNoV GI and GII replicate to high titers in zebrafish (Danio rerio) larvae; replication peaks at day 2 post infection and is detectable for at least 6 days. The virus (HuNoV GII.4) could be passaged from larva to larva two consecutive times. HuNoV is detected in cells of the hematopoietic lineage and the intestine, supporting the notion of a dual tropism. Antiviral treatment reduces HuNoV replication by >2 log(10), showing that this model is suited for antiviral studies. Zebrafish larvae constitute a simple and robust replication model that will largely facilitate studies of HuNoV biology and the development of antiviral strategies. Public Library of Science 2019-09-19 /pmc/articles/PMC6752765/ /pubmed/31536612 http://dx.doi.org/10.1371/journal.ppat.1008009 Text en © 2019 Van Dycke et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Van Dycke, Jana
Ny, Annelii
Conceição-Neto, Nádia
Maes, Jan
Hosmillo, Myra
Cuvry, Arno
Goodfellow, Ian
Nogueira, Tatiane C.
Verbeken, Erik
Matthijnssens, Jelle
de Witte, Peter
Neyts, Johan
Rocha-Pereira, Joana
A robust human norovirus replication model in zebrafish larvae
title A robust human norovirus replication model in zebrafish larvae
title_full A robust human norovirus replication model in zebrafish larvae
title_fullStr A robust human norovirus replication model in zebrafish larvae
title_full_unstemmed A robust human norovirus replication model in zebrafish larvae
title_short A robust human norovirus replication model in zebrafish larvae
title_sort robust human norovirus replication model in zebrafish larvae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6752765/
https://www.ncbi.nlm.nih.gov/pubmed/31536612
http://dx.doi.org/10.1371/journal.ppat.1008009
work_keys_str_mv AT vandyckejana arobusthumannorovirusreplicationmodelinzebrafishlarvae
AT nyannelii arobusthumannorovirusreplicationmodelinzebrafishlarvae
AT conceicaonetonadia arobusthumannorovirusreplicationmodelinzebrafishlarvae
AT maesjan arobusthumannorovirusreplicationmodelinzebrafishlarvae
AT hosmillomyra arobusthumannorovirusreplicationmodelinzebrafishlarvae
AT cuvryarno arobusthumannorovirusreplicationmodelinzebrafishlarvae
AT goodfellowian arobusthumannorovirusreplicationmodelinzebrafishlarvae
AT nogueiratatianec arobusthumannorovirusreplicationmodelinzebrafishlarvae
AT verbekenerik arobusthumannorovirusreplicationmodelinzebrafishlarvae
AT matthijnssensjelle arobusthumannorovirusreplicationmodelinzebrafishlarvae
AT dewittepeter arobusthumannorovirusreplicationmodelinzebrafishlarvae
AT neytsjohan arobusthumannorovirusreplicationmodelinzebrafishlarvae
AT rochapereirajoana arobusthumannorovirusreplicationmodelinzebrafishlarvae
AT vandyckejana robusthumannorovirusreplicationmodelinzebrafishlarvae
AT nyannelii robusthumannorovirusreplicationmodelinzebrafishlarvae
AT conceicaonetonadia robusthumannorovirusreplicationmodelinzebrafishlarvae
AT maesjan robusthumannorovirusreplicationmodelinzebrafishlarvae
AT hosmillomyra robusthumannorovirusreplicationmodelinzebrafishlarvae
AT cuvryarno robusthumannorovirusreplicationmodelinzebrafishlarvae
AT goodfellowian robusthumannorovirusreplicationmodelinzebrafishlarvae
AT nogueiratatianec robusthumannorovirusreplicationmodelinzebrafishlarvae
AT verbekenerik robusthumannorovirusreplicationmodelinzebrafishlarvae
AT matthijnssensjelle robusthumannorovirusreplicationmodelinzebrafishlarvae
AT dewittepeter robusthumannorovirusreplicationmodelinzebrafishlarvae
AT neytsjohan robusthumannorovirusreplicationmodelinzebrafishlarvae
AT rochapereirajoana robusthumannorovirusreplicationmodelinzebrafishlarvae