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Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning

Highly specific Cas9 nucleases derived from SpCas9 are valuable tools for genome editing, but their wide applications are hampered by a lack of knowledge governing guide RNA (gRNA) activity. Here, we perform a genome-scale screen to measure gRNA activity for two highly specific SpCas9 variants (eSpC...

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Detalles Bibliográficos
Autores principales:	Wang, Daqi, Zhang, Chengdong, Wang, Bei, Li, Bin, Wang, Qiang, Liu, Dong, Wang, Hongyan, Zhou, Yan, Shi, Leming, Lan, Feng, Wang, Yongming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753114/ https://www.ncbi.nlm.nih.gov/pubmed/31537810 http://dx.doi.org/10.1038/s41467-019-12281-8

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