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Prebiotic Phosphorylation of Uridine using Diamidophosphate in Aerosols
One of the most challenging fundamental problems in establishing prebiotically plausible routes for phosphorylation reactions using phosphate is that they are thermodynamically unfavorable in aqueous conditions. Diamidophosphate (DAP), a potentially prebiotically relevant compound, was shown to phos...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753121/ https://www.ncbi.nlm.nih.gov/pubmed/31537885 http://dx.doi.org/10.1038/s41598-019-49947-8 |
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author | Castañeda, A. D. Li, Z. Joo, T. Benham, K. Burcar, B. T. Krishnamurthy, R. Liotta, C. L. Ng, N. L. Orlando, T. M. |
author_facet | Castañeda, A. D. Li, Z. Joo, T. Benham, K. Burcar, B. T. Krishnamurthy, R. Liotta, C. L. Ng, N. L. Orlando, T. M. |
author_sort | Castañeda, A. D. |
collection | PubMed |
description | One of the most challenging fundamental problems in establishing prebiotically plausible routes for phosphorylation reactions using phosphate is that they are thermodynamically unfavorable in aqueous conditions. Diamidophosphate (DAP), a potentially prebiotically relevant compound, was shown to phosphorylate nucleosides in aqueous medium, albeit at a very slow rate (days/weeks). Here, we demonstrate that performing these reactions within an aerosol environment, a suitable model for the early Earth ocean-air interface, yields higher reaction rates when compared to bulk solution, thus overcoming these rate limitations. As a proof-of-concept, we demonstrate the effective conversion (~6.5–10%) of uridine to uridine-2′,3′-cyclophosphate in less than 1 h. These results suggest that aerosol environments are a possible scenario in which prebiotic phosphorylation could have occurred despite unfavorable rates in bulk solution. |
format | Online Article Text |
id | pubmed-6753121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67531212019-10-01 Prebiotic Phosphorylation of Uridine using Diamidophosphate in Aerosols Castañeda, A. D. Li, Z. Joo, T. Benham, K. Burcar, B. T. Krishnamurthy, R. Liotta, C. L. Ng, N. L. Orlando, T. M. Sci Rep Article One of the most challenging fundamental problems in establishing prebiotically plausible routes for phosphorylation reactions using phosphate is that they are thermodynamically unfavorable in aqueous conditions. Diamidophosphate (DAP), a potentially prebiotically relevant compound, was shown to phosphorylate nucleosides in aqueous medium, albeit at a very slow rate (days/weeks). Here, we demonstrate that performing these reactions within an aerosol environment, a suitable model for the early Earth ocean-air interface, yields higher reaction rates when compared to bulk solution, thus overcoming these rate limitations. As a proof-of-concept, we demonstrate the effective conversion (~6.5–10%) of uridine to uridine-2′,3′-cyclophosphate in less than 1 h. These results suggest that aerosol environments are a possible scenario in which prebiotic phosphorylation could have occurred despite unfavorable rates in bulk solution. Nature Publishing Group UK 2019-09-19 /pmc/articles/PMC6753121/ /pubmed/31537885 http://dx.doi.org/10.1038/s41598-019-49947-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Castañeda, A. D. Li, Z. Joo, T. Benham, K. Burcar, B. T. Krishnamurthy, R. Liotta, C. L. Ng, N. L. Orlando, T. M. Prebiotic Phosphorylation of Uridine using Diamidophosphate in Aerosols |
title | Prebiotic Phosphorylation of Uridine using Diamidophosphate in Aerosols |
title_full | Prebiotic Phosphorylation of Uridine using Diamidophosphate in Aerosols |
title_fullStr | Prebiotic Phosphorylation of Uridine using Diamidophosphate in Aerosols |
title_full_unstemmed | Prebiotic Phosphorylation of Uridine using Diamidophosphate in Aerosols |
title_short | Prebiotic Phosphorylation of Uridine using Diamidophosphate in Aerosols |
title_sort | prebiotic phosphorylation of uridine using diamidophosphate in aerosols |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753121/ https://www.ncbi.nlm.nih.gov/pubmed/31537885 http://dx.doi.org/10.1038/s41598-019-49947-8 |
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