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RNA polymerase II plays an active role in the formation of gene loops through the Rpb4 subunit

Gene loops are formed by the interaction of initiation and termination factors occupying the distal ends of a gene during transcription. RNAPII is believed to affect gene looping indirectly owing to its essential role in transcription. The results presented here, however, demonstrate a direct role o...

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Autores principales: Allepuz-Fuster, Paula, O’Brien, Michael J, González-Polo, Noelia, Pereira, Bianca, Dhoondia, Zuzer, Ansari, Athar, Calvo, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753479/
https://www.ncbi.nlm.nih.gov/pubmed/31304538
http://dx.doi.org/10.1093/nar/gkz597
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author Allepuz-Fuster, Paula
O’Brien, Michael J
González-Polo, Noelia
Pereira, Bianca
Dhoondia, Zuzer
Ansari, Athar
Calvo, Olga
author_facet Allepuz-Fuster, Paula
O’Brien, Michael J
González-Polo, Noelia
Pereira, Bianca
Dhoondia, Zuzer
Ansari, Athar
Calvo, Olga
author_sort Allepuz-Fuster, Paula
collection PubMed
description Gene loops are formed by the interaction of initiation and termination factors occupying the distal ends of a gene during transcription. RNAPII is believed to affect gene looping indirectly owing to its essential role in transcription. The results presented here, however, demonstrate a direct role of RNAPII in gene looping through the Rpb4 subunit. 3C analysis revealed that gene looping is abolished in the rpb4Δ mutant. In contrast to the other looping-defective mutants, rpb4Δ cells do not exhibit a transcription termination defect. RPB4 overexpression, however, rescued the transcription termination and gene looping defect of sua7-1, a mutant of TFIIB. Furthermore, RPB4 overexpression rescued the ssu72-2 gene looping defect, while SSU72 overexpression restored the formation of gene loops in rpb4Δ cells. Interestingly, the interaction of TFIIB with Ssu72 is compromised in rpb4Δ cells. These results suggest that the TFIIB–Ssu72 interaction, which is critical for gene loop formation, is facilitated by Rpb4. We propose that Rpb4 is promoting the transfer of RNAPII from the terminator to the promoter for reinitiation of transcription through TFIIB–Ssu72 mediated gene looping.
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spelling pubmed-67534792019-09-25 RNA polymerase II plays an active role in the formation of gene loops through the Rpb4 subunit Allepuz-Fuster, Paula O’Brien, Michael J González-Polo, Noelia Pereira, Bianca Dhoondia, Zuzer Ansari, Athar Calvo, Olga Nucleic Acids Res Gene regulation, Chromatin and Epigenetics Gene loops are formed by the interaction of initiation and termination factors occupying the distal ends of a gene during transcription. RNAPII is believed to affect gene looping indirectly owing to its essential role in transcription. The results presented here, however, demonstrate a direct role of RNAPII in gene looping through the Rpb4 subunit. 3C analysis revealed that gene looping is abolished in the rpb4Δ mutant. In contrast to the other looping-defective mutants, rpb4Δ cells do not exhibit a transcription termination defect. RPB4 overexpression, however, rescued the transcription termination and gene looping defect of sua7-1, a mutant of TFIIB. Furthermore, RPB4 overexpression rescued the ssu72-2 gene looping defect, while SSU72 overexpression restored the formation of gene loops in rpb4Δ cells. Interestingly, the interaction of TFIIB with Ssu72 is compromised in rpb4Δ cells. These results suggest that the TFIIB–Ssu72 interaction, which is critical for gene loop formation, is facilitated by Rpb4. We propose that Rpb4 is promoting the transfer of RNAPII from the terminator to the promoter for reinitiation of transcription through TFIIB–Ssu72 mediated gene looping. Oxford University Press 2019-09-26 2019-07-15 /pmc/articles/PMC6753479/ /pubmed/31304538 http://dx.doi.org/10.1093/nar/gkz597 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Gene regulation, Chromatin and Epigenetics
Allepuz-Fuster, Paula
O’Brien, Michael J
González-Polo, Noelia
Pereira, Bianca
Dhoondia, Zuzer
Ansari, Athar
Calvo, Olga
RNA polymerase II plays an active role in the formation of gene loops through the Rpb4 subunit
title RNA polymerase II plays an active role in the formation of gene loops through the Rpb4 subunit
title_full RNA polymerase II plays an active role in the formation of gene loops through the Rpb4 subunit
title_fullStr RNA polymerase II plays an active role in the formation of gene loops through the Rpb4 subunit
title_full_unstemmed RNA polymerase II plays an active role in the formation of gene loops through the Rpb4 subunit
title_short RNA polymerase II plays an active role in the formation of gene loops through the Rpb4 subunit
title_sort rna polymerase ii plays an active role in the formation of gene loops through the rpb4 subunit
topic Gene regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753479/
https://www.ncbi.nlm.nih.gov/pubmed/31304538
http://dx.doi.org/10.1093/nar/gkz597
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