Single-molecule detection of deoxyribonucleoside triphosphates in microdroplets

A new approach to single-molecule DNA sequencing in which dNTPs, released by pyrophosphorolysis from the strand to be sequenced, are captured in microdroplets and read directly could have substantial advantages over current sequence-by-synthesis methods; however, there is no existing method sensitiv...

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Detalles Bibliográficos
Autores principales: Breiner, Boris, Johnson, Kerr, Stolarek, Magdalena, Silva, Ana-Luisa, Negrea, Aurel, Bell, Neil M, Isaac, Tom H, Dethlefsen, Mark, Chana, Jasmin, Ibbotson, Lindsey A, Palmer, Rebecca N, Bush, James, Dunning, Alexander J, Love, David M, Pachoumi, Olympia, Kelly, Douglas J, Shibahara, Aya, Wu, Mei, Sosna, Maciej, Dear, Paul H, Tolle, Fabian, Petrini, Edoardo, Amasio, Michele, Shelford, Leigh R, Saavedra, Monica S, Sheridan, Eoin, Kuleshova, Jekaterina, Podd, Gareth J, Balmforth, Barnaby W, Frayling, Cameron A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753480/
https://www.ncbi.nlm.nih.gov/pubmed/31318971
http://dx.doi.org/10.1093/nar/gkz611

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