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Post-transcriptional gene silencing triggers dispensable DNA methylation in gene body in Arabidopsis

Spontaneous post-transcriptional silencing of sense transgenes (S-PTGS) is established in each generation and is accompanied by DNA methylation, but the pathway of PTGS-dependent DNA methylation is unknown and so is its role. Here we show that CHH and CHG methylation coincides spatially and temporal...

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Autores principales: Taochy, Christelle, Yu, Agnès, Bouché, Nicolas, Bouteiller, Nathalie, Elmayan, Taline, Dressel, Uwe, Carroll, Bernard J, Vaucheret, Hervé
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753489/
https://www.ncbi.nlm.nih.gov/pubmed/31372641
http://dx.doi.org/10.1093/nar/gkz636
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author Taochy, Christelle
Yu, Agnès
Bouché, Nicolas
Bouteiller, Nathalie
Elmayan, Taline
Dressel, Uwe
Carroll, Bernard J
Vaucheret, Hervé
author_facet Taochy, Christelle
Yu, Agnès
Bouché, Nicolas
Bouteiller, Nathalie
Elmayan, Taline
Dressel, Uwe
Carroll, Bernard J
Vaucheret, Hervé
author_sort Taochy, Christelle
collection PubMed
description Spontaneous post-transcriptional silencing of sense transgenes (S-PTGS) is established in each generation and is accompanied by DNA methylation, but the pathway of PTGS-dependent DNA methylation is unknown and so is its role. Here we show that CHH and CHG methylation coincides spatially and temporally with RDR6-dependent products derived from the central and 3′ regions of the coding sequence, and requires the components of the RNA-directed DNA methylation (RdDM) pathway NRPE1, DRD1 and DRM2, but not CLSY1, NRPD1, RDR2 or DCL3, suggesting that RDR6-dependent products, namely long dsRNAs and/or siRNAs, trigger PTGS-dependent DNA methylation. Nevertheless, none of these RdDM components are required to establish S-PTGS or produce a systemic silencing signal. Moreover, preventing de novo DNA methylation in non-silenced transgenic tissues grafted onto homologous silenced tissues does not inhibit the triggering of PTGS. Overall, these data indicate that gene body DNA methylation is a consequence, not a cause, of PTGS, and rule out the hypothesis that a PTGS-associated DNA methylation signal is transmitted independent of a PTGS signal.
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spelling pubmed-67534892019-09-25 Post-transcriptional gene silencing triggers dispensable DNA methylation in gene body in Arabidopsis Taochy, Christelle Yu, Agnès Bouché, Nicolas Bouteiller, Nathalie Elmayan, Taline Dressel, Uwe Carroll, Bernard J Vaucheret, Hervé Nucleic Acids Res Gene regulation, Chromatin and Epigenetics Spontaneous post-transcriptional silencing of sense transgenes (S-PTGS) is established in each generation and is accompanied by DNA methylation, but the pathway of PTGS-dependent DNA methylation is unknown and so is its role. Here we show that CHH and CHG methylation coincides spatially and temporally with RDR6-dependent products derived from the central and 3′ regions of the coding sequence, and requires the components of the RNA-directed DNA methylation (RdDM) pathway NRPE1, DRD1 and DRM2, but not CLSY1, NRPD1, RDR2 or DCL3, suggesting that RDR6-dependent products, namely long dsRNAs and/or siRNAs, trigger PTGS-dependent DNA methylation. Nevertheless, none of these RdDM components are required to establish S-PTGS or produce a systemic silencing signal. Moreover, preventing de novo DNA methylation in non-silenced transgenic tissues grafted onto homologous silenced tissues does not inhibit the triggering of PTGS. Overall, these data indicate that gene body DNA methylation is a consequence, not a cause, of PTGS, and rule out the hypothesis that a PTGS-associated DNA methylation signal is transmitted independent of a PTGS signal. Oxford University Press 2019-09-26 2019-08-02 /pmc/articles/PMC6753489/ /pubmed/31372641 http://dx.doi.org/10.1093/nar/gkz636 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Gene regulation, Chromatin and Epigenetics
Taochy, Christelle
Yu, Agnès
Bouché, Nicolas
Bouteiller, Nathalie
Elmayan, Taline
Dressel, Uwe
Carroll, Bernard J
Vaucheret, Hervé
Post-transcriptional gene silencing triggers dispensable DNA methylation in gene body in Arabidopsis
title Post-transcriptional gene silencing triggers dispensable DNA methylation in gene body in Arabidopsis
title_full Post-transcriptional gene silencing triggers dispensable DNA methylation in gene body in Arabidopsis
title_fullStr Post-transcriptional gene silencing triggers dispensable DNA methylation in gene body in Arabidopsis
title_full_unstemmed Post-transcriptional gene silencing triggers dispensable DNA methylation in gene body in Arabidopsis
title_short Post-transcriptional gene silencing triggers dispensable DNA methylation in gene body in Arabidopsis
title_sort post-transcriptional gene silencing triggers dispensable dna methylation in gene body in arabidopsis
topic Gene regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753489/
https://www.ncbi.nlm.nih.gov/pubmed/31372641
http://dx.doi.org/10.1093/nar/gkz636
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